Veterinary Drug Mixture Standard Solutions by Drug Classification

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Drugs used in animals are called veterinary drugs. Veterinary drugs are used for various animals such as pet animals, industrial animals, fish and poultry. Veterinary drugs are used to prevent and treatment of animal diseases, but in industrial animals including livestock, the drug remains in foods (meat, eggs, milk, seafood, etc.) which derived from dosed animals. Therefore, residual standard values ​​have been set so that residual veterinary drugs do not damage human health. FUJIFILM Wako provides Veterinary Drug Mixture Standard, Veterinary Drug Mixture Standard Solutions by drug classification and PL series.
Veterinary drug mixture standard Solutions by drug classification are mixed with veterinary drugs by drug classification (quinolone drug, sulfa drug, macrolide, hormone drug).

Quinolone

LC/MS Chromatogram

00373_img01.png
Peak No. Constituent Monitoring Ions
(m/z)
Mode Peak No. Constituent Monitoring Ions
(m/z)
Mode
1 Marbofloxacin 363 + 8 Sarafloxacin* 386 +
2 Norfloxacin 320 + 9 Difloxacin 400 +
3 Ofloxacin 362 + 10 Oxolinic Acid 262 +
4 Ciprofloxacin* 332 + 11 Nalidixic Acid 233 +
5 Danofloxacin * 358 + 12 Flumequine 262 +
6 Enrofloxacin 360 + 13 Piromidic Acid 289 +
7 Orbifloxacin 396 +

*The preparation concentration is converted to 20 µg/mL as free base.

Analysis conditions

[ Instrument ]
Shimadzu LC/MS-2020

[HPLC]
Column: Wakopak® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm
Column temperature: 40 ℃
Eluent: A) 0.1 % HCOOH in H2O
B) CH3CN
Gradient:

Time (min) B conc. (%)
0-10 1-10
10-35 10-100
35-40 100

Flow rate: 0.2 mL/min

[MS]
Ionization: ESI
Mode: TIC

Sulfonamide + Antifolate

LC/MS Chromatogram

00373_img02.png
Peak No. Constituent Monitoring Ions
(m/z)
Mode Peak No. Constituent Monitoring Ions
(m/z)
Mode
Precursor Product Precursor Product
1 Sulfanilamide 173 156 + 15 Pyrimethamine 249 233 +
2 Sulfacetamide 215 156 + 16 Sulfamonomethoxine 281 92 +
3 Diaverdine 261 245 + 17 Sulfachlorpyridazine* 285 156 +
4 Sulfisomidine 279 124 + 18 Sulfamethoxazole 254 92 +
5 Sulfadiazine 251 156 + 19 Sulfadoxine 311 156 +
6 Trimethoprim 291 230 + 20 Sulfatroxazole 268 92 +
7 Ormetoprim 275 259 + 21 Sulfaethoxypyridazine 295 156 +
8 Sulfathiazole 256 156 + 22 Sulfisoxazole 268 92 +
9 Sulfapyridine 250 156 + 23 Sulfabenzamide 277 156 +
10 Sulfamerazine 265 92 + 24 Sulfadimethoxine 311 156 +
11 Sulfisozole* 240 156 + 25 Sulfaquinoxaline* 301 156 +
12 Sulfametoxydiazine 281 92 + 26 Sulfabromomethazine 359 92 +
13 Sulfadimidine 279 92 + 27 Sulfanitran 336 156
14 Sulfamethoxypyridazine 281 156 +

*The preparation concentration is converted to 20 µg/mL as free base.

Analysis conditions

[ Instrument ]
Shimadzu LC/MS-8040

[HPLC]
Column: Wakopak® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm
Column temperature: 40 ℃
Eluent: A) 0.01 % HCOOH in H2O
B) CH3CN
Gradient:

Time (min) B conc. (%)
0-25 2-45
25-35 45

Flow rate: 0.2 mL/min

[MS]
Ionization: ESI
Mode:MRM

Macrolide

LC/MS Chromatogram

00373_img03.png
Peak No. Constituent Monitoring Ions
(m/z)
Mode Peak No. Constituent Monitoring Ions
(m/z)
Mode
1 Neospiramycin I 350 + 5 Tylosin 916 +
2 Spiramycin I 422 + 6 Tiamulin* 494 +
3 Tilmicosin (mixture of isomers) 870 + 7 Leucomycin A5 772 +
4 Erythromycin A 734 +

*The preparation concentration is converted to 20 µg/mL as free base.

Analysis conditions

[ Instrument ]
Shimadzu LC-20AD

[HPLC]
Column: Wakopak® Ultra C18-3 2.0 × 100 mm
Column temperature: 40 ℃
Eluent: A) 0.5 % HCOOH in H2O
B) 0.5 % HCOOH in CH3CN
Gradient:

Time (min) B conc. (%)
0-15 15-50
15-20 50

Flow rate: 0.2 mL/min

[MS]
Ionization: ESI
Mode: TIC

Hormone

LC/MS Chromatogram

00373_img04.png
Peak No. Constituent Monitoring Ions
(m/z)
Mode Peak No. Constituent Monitoring Ions
(m/z)
Mode
1 Prednisolone 361 + 4 α-Trenbolone 271 +
2 Methylprednisolone 375 + 5 Zeranol 323 +
3 β-Trenbolone 271 + 6 Clostebol 323 +

Analysis conditions

[ Instrument ]
Shimadzu LC/MS-2020

[HPLC]
Column: Wakopak® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm
Column temperature: 40 ℃
Eluent: A) 0.1 % HCOOH in H2O
B) CH3CN
Gradient:

Time (min) B conc. (%)
0-20 30-70
20-30 70

Flow rate: 0.2 mL/min

[MS]
Ionization: ESI
Mode: TIC

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Mixed Standard Solutions

Standard Solutions

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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