JCRB cell lines in combination with Fujifilm Wako culture and freezing media provide necessary tools for disease research
Overview
Cells are alive and require continuous culture and passaging for their maintenance. Long-term cell culture is costly, time-consuming and carries the risk not only of contamination with bacteria or other cell lines and interruption of culture due to equipment failure, but also of changes in the inherent characteristics of the cell line, such as cell morphology, growth potential and sensitivity to bioactive substances. Cell preservation method is one of the most important technologies in the field of cell therapy to protect cells from these risks and reduce the unnecessary cost.
For this purpose, Fujifilm Wako provides distinctive JCRB cell line products, culture, and cryopreservation media products.
JCRB Cell Lines
JCRB (Japanese Collection of Research Bioresources) is one of the most comprehensive cell banks in the world for pure and applied science, spanning many fields of research across the globe.
Fujifilm Wako has teamed up with the National Institute of Biomedical Innovation to bring you access to over 1,600 cell lines, which can be used not only as a monolayer cell culture, but also as a 3D cell culture.
Most popular JCRB cell line products
| JCRB No. | Cell-line name | Profile |
|---|---|---|
| JCRB0403 | HuH-7 | Well differentiated human hepatocellular carcinoma cell line |
| IFO50041 | MBT2 | Bladder carcinoma |
| JCRB0834 | NUGC-4 | Stomach adenocarcinoma, poorly differentiated, signet ring cell carcinoma |
| JCRB0098 | KURAMOCHI | Ovarian undifferentiated carcinoma, ascites |
| JCRB1020 | RERF-LC-Ad1 | Human lung cancer cell line, adenocarcinoma |
| JCRB1043 | OVISE | Human cell line derived from ovarian tumor |
Applications of JCRB cell lines
1. Toxicology and Drug Screening
JCRB0134 (MCF-7) cell lines are used as valuable models for toxicology and drug discovery. They retain their genome over time and can be used to perform drug screening and facilitate patient specific drug development.
2. Benefit of Variety of Ethnic Background in Patient Targeted Cancer Therapies
JCRB offers a highly representative panel of cell lines in terms of ethnic and gender diversity, which could improve the success rate in identifying effective cancer therapeutics in cell culture models and thereby reduce drug failures in diverse human populations.
JCRB cell lines related cell culture & freezing media
Fujifilm Wako offers cell culture and freezing media to support a wide range of research using JCRB cell line products.
BAMBANKER® Medium series
BAMBANKER™ Direct offers enhanced operability. A significant advantage of this medium is its elimination of the need for centrifugation during cell collection, simplifying the preservation process. This feature streamlines cell preparation, saving time and reducing the potential for cell damage associated with centrifugation.
Features
- Ready-to-use medium for preservation of cells
- Usable without diluting
- NO program freezer is required
- Rapid and long-term freezing and preservation in a deep freezer (-80°C) Serum-free
Applications
Comparison of Bambanker® with other relevant products
BAMBANKER® products list
| Product Code | Product name | Package size |
|---|---|---|
| 302-14686 | BAMBANKER® | 100 mL |
| 306-95921 | BAMBANKER® Direct | 20 mL |
Cancer cell culturing media
F-PDO, developed by the Fukushima Translational Research Project, refers to a suite of patient-derived tumor organoids (PDOs) recently established from various types of tumor tissues, including but not limited to human lung, ovarian, and uterine tumors1,2. These novel PDOs are part of an ongoing effort to broaden the scope of cancer research. Notably, this product is designed for culturing F-PDOs and has demonstrated the capability to maintain PDO cultures for over six months. During this extended period, the PDOs have shown the ability to form cell clusters that retain similar morphological characteristics to their original tumor tissues, even after prolonged growth under specific culture conditions. This sustained morphological consistency suggests that F-PDOs could potentially replicate similar functions when used in the culture of established cancer cell lines. This feature underlines the potential of F-PDOs as a reliable tumor organoid model for cancer research and therapeutic development.
Cancer cell culturing media products list
| Product Code | Product Name | Size |
|---|---|---|
| 035-25735 | Cancer Cell Expansion Media | 500 mL |
| 032-25745 | Cancer Cell Expansion Media plus | 500 mL |
Citations
- Tamura H, et al.: Oncol Rep. 2018 Aug;40(2):635-646.
- Higa A, et al.: J Vis Exp. 2021 Jun 14;(172).
