HPLC Column for Environment Analysis

Wakopak^® Wakosil^® DNPH

n- and iso-butyraldehydes can be separated by HPLC.

Column	：Wakopak® Wakosil® DNPH (4.6 mm x 250 mm)	Sample：	1) Formaldehyde-2,4-DNPH 2) Acetaldehyde-2,4-DNPH 3) Propionaldehyde-2,4-DNPH 4) Acrolein-2,4-DNPH 5) Acetone-2,4-DNPH 6) iso-Butyraldehyde-2,4-DNPH 7) n-Butyraldehyde-2,4-DNPH 8) Crotonaldehyde-2,4-DNPH 9) iso-Valeraldehyde-2,4-DNPH 10) n-Valeraldehyde-2,4-DNPH 11) Benzaldehyde-2,4-DNPH 12) Hexaldehyde-2,4-DNPH 13) o-Tolualdehyde-2,4-DNPH 14) m-Tolualdehyde-2,4-DNPH 15) p-Tolualdehyde-2,4-DNPH 16) 2,5-Dimethylbenzaldehyde-2,4-DNPH each 0.625 µg/mL (as aldehyde, ketone)
Eluent	：A) Wakosil® DNPH Eluent A 　B) Wakosil® DNPH Eluent B 　0 - 16 min.　B 10 % 　16 - 35 min.　B 10 - 90 % 　35 - 45 min.　B 90 %
Flow Rate	：0.6 mL/min. at 37 ℃
Detection	：UV 360 nm, 0.016 AUFS

Wakopak^® Wakosil^® DNPH-II

1. Blank

One Presep^®-C DNPH cartridge eluted with acetonitrile and diluted to 5 mL.

2. Example of analysis of indoor air sample

30 L of air in the laboratory sampled at a flow rate of 0.5 L/min using Presep^®-C DNPH and eluted in the same manner as described above.​

3. Reference standard ​

Chromatogram of the reference standard of 15 DNPH-derivatized aldehydes​

• Column	：Wakopak® Wakosil® DNPH-II (4.6 mm x 150 mm)
  Eluent	：A) Wakosil® DNPH-Ⅱ Eluent A 　B）Wakosil® DNPH-Ⅱ Eluent B 　0 - 10 min　 B conc. 0-100 % 　10 - 16 min　B conc. 100 %
  Flow Rate	：1.0 mL/min. at 35 ℃
  Detection	：UV 360 nm
  Injection Volume	：10 µL

• Sample：

  1) Formaldehyde-2,4-DNPH 2) Acetaldehyde-2,4-DNPH 3) Acetone-2,4-DNPH 4) Acrolein-2,4-DNPH 5) Propionaldehyde-2,4-DNPH 6) Crotonaldehyde-2,4-DNPH 7) n-,iso-Butyraldehyde-2,4-DNPH 8) Benzaldehyde-2,4-DNPH 9) iso-Valeraldehyde-2,4-DNPH 10) n-Valeraldehyde-2,4-DNPH 11) o-Tolualdehyde-2,4-DNPH 12) m-Tolualdehyde-2,4-DNPH 13) p-Tolualdehyde-2,4-DNPH 14) Hexaldehyde-2,4-DNPH 15) 2,5-Dimethylbenzaldehyde-2,4-DNPH each 0.1 μg/mL (as aldehyde,ketone)