Columns for analysis of DNPH-derivatized aldehydes and ketones

HPLC Column for Environment Analysis

Wakopak® Wakosil® DNPH, Wakopak® Wakosil® DNPH-Ⅱ

These are columns for the HPLC analysis of aldehydes and ketones in the atmosphere. These special columns are optimized for the simultaneous analysis of 2,4-dinitrophenylhydrazone (DNPH)-derivatized aldehydes and ketones. Aldehydes are subject to regulation and measurement as hazardous air pollutants under the Air Pollution Control Act, Offensive Odor Control Law, etc.​

​Wakopak® Wakosil® DNPH can separate n- and iso-butyraldehydes, which are difficult to separate with general HPLC columns. It can analyze the same components as those analyzed by GC.

Wakopak® Wakosil® DNPH-II can separate 15 components regulated by the US EPA within 20 minutes.​

Example of analysis of 16 DNPH aldehydes

Wakopak® Wakosil® DNPH

n- and iso-butyraldehydes can be separated by HPLC.

Column :Wakopak® Wakosil® DNPH (4.6 mm x 250 mm) Sample: 1) Formaldehyde-2,4-DNPH
2) Acetaldehyde-2,4-DNPH
3) Propionaldehyde-2,4-DNPH
4) Acrolein-2,4-DNPH
5) Acetone-2,4-DNPH
6) iso-Butyraldehyde-2,4-DNPH
7) n-Butyraldehyde-2,4-DNPH
8) Crotonaldehyde-2,4-DNPH
9) iso-Valeraldehyde-2,4-DNPH
10) n-Valeraldehyde-2,4-DNPH
11) Benzaldehyde-2,4-DNPH
12) Hexaldehyde-2,4-DNPH
13) o-Tolualdehyde-2,4-DNPH
14) m-Tolualdehyde-2,4-DNPH
15) p-Tolualdehyde-2,4-DNPH
16) 2,5-Dimethylbenzaldehyde-2,4-DNPH
each 0.625 µg/mL (as aldehyde, ketone)
Eluent :A) Wakosil® DNPH Eluent A
 B) Wakosil® DNPH Eluent B
 0 - 16 min. B 10 %
 16 - 35 min. B 10 - 90 %
 35 - 45 min. B 90 %
Flow Rate :0.6 mL/min. at 37 ℃
Detection :UV 360 nm, 0.016 AUFS

Analysis of 15 DNPH aldehydes (rapid analysis)

Wakopak® Wakosil® DNPH-II

1. Blank

One Presep®-C DNPH cartridge eluted with acetonitrile and diluted to 5 mL.

2. Example of analysis of indoor air sample

30 L of air in the laboratory sampled at a flow rate of 0.5 L/min using Presep®-C DNPH and eluted in the same manner as described above.​

3. Reference standard ​

Chromatogram of the reference standard of 15 DNPH-derivatized aldehydes​

  • Column :Wakopak® Wakosil® DNPH-II (4.6 mm x 150 mm)
    Eluent :A) Wakosil® DNPH-Ⅱ Eluent A
     B)Wakosil® DNPH-Ⅱ Eluent B
     0 - 10 min  B conc. 0-100 %
     10 - 16 min B conc. 100 %
    Flow Rate :1.0 mL/min. at 35 ℃
    Detection :UV 360 nm
    Injection Volume :10 µL
  • Sample: 1) Formaldehyde-2,4-DNPH
    2) Acetaldehyde-2,4-DNPH
    3) Acetone-2,4-DNPH
    4) Acrolein-2,4-DNPH
    5) Propionaldehyde-2,4-DNPH
    6) Crotonaldehyde-2,4-DNPH
    7) n-,iso-Butyraldehyde-2,4-DNPH
    8) Benzaldehyde-2,4-DNPH
    9) iso-Valeraldehyde-2,4-DNPH
    10) n-Valeraldehyde-2,4-DNPH
    11) o-Tolualdehyde-2,4-DNPH
    12) m-Tolualdehyde-2,4-DNPH
    13) p-Tolualdehyde-2,4-DNPH
    14) Hexaldehyde-2,4-DNPH
    15) 2,5-Dimethylbenzaldehyde-2,4-DNPH
    each 0.1 μg/mL (as aldehyde,ketone)

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Column for HPLC

DNPH Eluent

Mixture Standard Solution

Solid-phase extraction column for pretreatment


For research use or further manufacturing use only. Not for use in diagnostic procedures.

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