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SPE Column Polymer-based Mix Mode (Reverse-phase + Ion Exchange)

Cation exchange
・Presep® RPP-SCX
・Presep® RPP-WCX

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Presep® RPP-ion exchange column is a pretreatment column for solid-phase extraction, consisting of the divinylbenzene-methacrylate polymer with ion exchange group. It has both reverse-phase and ion exchange modes and is suitable for the selective extraction of acidic and basic compounds.
If Presep® RPP-ion exchange columns are preconditioned using two protocols, the most suitable column can be selected. These protocols are most suitable for the selective extraction of target substances and initial examination of contaminant component removal.

Product Code Product Name Filler Content (mg/cartridge) Feature Functional Group Use
291-34921 Presep® RPP-SCX 60/3 mL Reverse-phase-cation exchange Sulfonic acid group Selective extraction of basic compounds
292-34831 Presep® RPP-WCX 60/3 mL rreverse-phase-weak cation exchange Carboxyl group Selective extraction of strongly basic compounds

Example of operation

Protocols Presep® RPP-WCX Presep® RPP-SCX
1.Conditioning Methanol→Water
2.Sample loading Phosphate buffer (pH 7.0) (*Adjust to the pH suitable for the purpose of extraction)
3.Washing Water
4.Extraction 1 Methanol
5.Extraction 2 Methanol containing 2-5% formic acid Methanol containing 2-5% ammonia
Final eluate Strongly basic compounds Basic compounds

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Features of Presep® RPP-SCX

Solid-phase extraction column made by filling a carrier in which a cation exchange group (sulfonic acid group) is bonded to a polymer having hydrophilic and hydrophobic groups.
It selectively extracts basic compounds.

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Features of Presep® RPP-WCX

Solid-phase extraction column made by filling a carrier in which a weak cation exchange group (carboxyl group) is bonded to a polymer having hydrophilic and hydrophobic groups.It selectively extracts strongly basic compounds.

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