HPLC Column for Combinatorial Chemistry "Wakopak^® Combi Series"

Wakopak^® Combi ODS series adopts high-purity ODS (5 μm, particle size). They can be applied to a wide-range of compounds, including hydrophilic/hydrophobic compounds and metal-coordination compounds.

Wakopak^® Combi ODS series are designed to minimize pressure loss to support gradient methods that enable separation in a short time. Wakopak^® Combi ODS fast series uses a packing agent with a particle size of 3 μm to achieve further reduction of column length and analysis time.

Column	：Wakopak® Combi ODS fast 　(4.6 mm x 30 mm)	Wakopak® Combi ODS (4.6 mm x 50 mm)
Flow Rate	：3.0 mL/min.	3.0 mL/min.
Gradient	：0 - 2.5 min.　B 0 - 80% 　2.5 - 3 min.　B 80%	0 - 3.5 min.　B 0 - 80% 3.5 - 4.5 min.　B 80%
Eluent	：A) CH3CN/H2O = 5/59(v/v), 0.05% TFA 　B) CH3CN/H2O = 95/5(v/v), 0.05% TFA
Temp	：R.T.
Detection	：UV 280 nm 2.048AUFS
Sample	：1) p-Hydroxybenzoic Acid 　2) Methyl p-hydroxybenzoate 　3) Salicylic acid 　4) Ethyl p-hydroxybenzoate 　5) Propyl p-hydroxybenzoate 　6) Methyl salicylate	7) Butyl p-hydroxybenzoate 8) Ethyl salicylate 9) Propyl salicylate 10) Butyl salicylate 　each 1 mg/mL
Injection Volume	：5 µL

Wakopak^® Combi CN series adopts packing agent made by introducing the cyanopropyl group to high-purity silica gel with a particle size of 5 μm, followed by end capping. Compared with ODS (C18) column, the hydrophilic compounds feature high retention, whereas the hydrophobic compounds feature low retention.

Column	：Wakopak® Combi CN 　(4.6 mm x 50 mm)	Wakopak® Combi ODS (4.6 mm x 50 mm)
Flow Rate	：2.0 mL/min.	2.0 mL/min.
Eluent	：A) CH3CN/H2O = 5/59(v/v), 0.05% TFA 　B) CH3CN/H2O = 95/5(v/v), 0.05% TFA 　0 - 5.0 min.　B 0 - 50% 　5.0 min.　B 50%
Temp	：R.T.
Detection	：UV 254 nm 2.048AUFS
Sample	：1) p-Hydroxybenzoic Acid 　2) Methyl p-hydroxybenzoate 　3) Ethyl p-hydroxybenzoate	4) Propyl p-hydroxybenzoate 　5) Butyl p-hydroxybenzoate 　　each 0.2 mg/mL