Wakopak^® Wakosil^®-Ⅱ 5SIL-AQ

• Excellent analysis of highly polar and basic compounds
• Use of high-purity silica gel: to suppress the influence of metal impurities
• Easier to apply to LC/MS analysis: Analysis conditions that use high concentration of organic solvent, and do not use ion-pair reagents can be set.

Analysis of nucleosides

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• Column Eluent Flow Rate Detection Injection

  ：Wakopak® Wakosil®-Ⅱ 5SIL-AQ (4.6 mm×250 mm) ：CH3CN/0.1M HCOONH4 (pH 3.0) = 85/15 (v/v) ：1.0 mL/min. at 40℃ ：UV 260 nm ：each 0.1 mg/mL (H2O), 10 μL

　

Analysis of tricyclic antidepressant (imipramine)

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• Column Eluent Flow Rate Detection Injection

  ：Wakopak® Wakosil®-Ⅱ 5SIL-AQ (4.6 mm×250 mm) ：CH3CN/0.1M HCOONH4 (pH 3.0) = 85/15 (v/v) ：1.0 mL/min. at 40℃ ：UV 254 nm ：each 0.2 mg/mL (EtOH), 10 μL

　

Analysis of acid substances (ascorbic acid)

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• Column Eluent Flow Rate Detection Injection

  ：Wakopak® Wakosil®-Ⅱ 5SIL-AQ (4.6 mm×250 mm) ：CH3CN/H2O/H3PO4=97/2/1（v/v/v） ：1.0 mL/min. at 40℃ ：UV 210 nm ：each 1.0 mg/mL (90%CH3CN), 5 μL

　

Analysis of crude drug ingredients (1)

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• Column Eluent Flow Rate Detection Injection

  ：Wakopak® Wakosil®-Ⅱ 5SIL-AQ (4.6 mm×250 mm) ：CH3CN/0.1M HCOONH4 (pH 3.0) = 95/5 (v/v) ：1.0 mL/min. at 40℃ ：UV 345 nm ：each 0.2 mg/mL (90% CH3CN), 5 μL

　

Analysis of crude drug ingredients (2)

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• Column Eluent Flow Rate Detection Injection

  ：Wakopak® Wakosil®-Ⅱ 5SIL-AQ (4.6 mm×250 mm) ：CH3CN/0.1M HCOONH4 (pH 3.0) = 85/15 (v/v) ：1.0 mL/min. at 40℃ ：UV 254 nm ：each 1 mg/mL (CH3CN), 10 μL

　

Analysis of crude drug ingredients (3)

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• Column Eluent Flow Rate Detection Injection

  ：Wakopak® Wakosil-Ⅱ® 5SIL-AQ (4.6 mm×250 mm) ：CH3CN/0.1M HCOONH4 (pH 3.0)= 80/20 (v/v) ：1.0 mL/min. at 35℃ ：UV 210 nm ：each 0.5 mg/mL (CH3CN), 5 μL