Wakopak® Wakosil® SIL Series
Wakopak® Wakosil® SIL Series use two types of silica gel packing agent: the high-adsorbing type, Wakosil® SIL, with a pore size of 6 nm and the low-adsorbing type, Wakosil® SIL-120, with a pore size of 12 nm. Their specific surface areas are 500 m2/g and 300 m2/g, respectively. The larger the specific surface area, the stronger the sample retention and the higher the resolution. Two adsorption quantities can be selected for more efficient analysis.
Types and Physical Properties of Packing Agents
| Packing Agents | Average Particle Size (µm) |
Average Pore Size (nm) |
Specific Surface Area (m2/g) |
Pore Volume (mL/g) |
|
|---|---|---|---|---|---|
| High-adsorbing Type | Wakosil® 5SIL | 5 | 6 | 500 | 0.75 |
| Wakosil® 10SIL | 10 | 6 | 500 | 0.75 | |
| Low-adsorbing Type | Wakosil® 5SIL-120 | 5 | 12 | 300 | 1.0 |
| Wakosil® 7SIL-120 | 7 | 12 | 300 | 1.0 |
Applications
Vitamin E homologues analysis
| Column Size Eluent Flow Rate Detection Injection Volume |
: 4.6 mm x 150 mm : n-Hexane/DIPE=90/10 (v/v) : 2.0 mL/min. at R.T. : UV 295 nm 0.32AUFS : 3 μL |
Corticosteroids analysis
-
Column
Eluent
Flow Rate
Detection: Wakopak® Wakosil® 5SIL (4.6 mm x 150 mm)
: n-Hexane/CHCl3/EtOH=7/2/1 (v/v/v)
: 1.0 mL/min. at R.T.
: UV 250 nm 0.04AUFS
Simultaneous analysis of five aflatoxins
-
Column
Eluent
Flow Rate
Detection
Injection Volume: Wakopak® Wakosil® 5SIL (4.6 mm x 250 mm)
: Toluene/Ethyl Acetate/Formic Acid/CH3OH=50/5/1/2 (v/v/v/v)
: 1.0 mL/min. at R.T.
: FL λex 365 nm, λem 425 nm
: 10 μL
Analysis of chlorophyll a and b
-
Column
Eluent
Flow Rate
Detection：Wakopak® Wakosil® 5SIL-120 (4.6 mm x 150 mm)
：n-Hexane/2-Propanol=98.5/1.5 (v/v)
：1.0 mL/min. at 0℃
：0→6 min. 430 nm
6 min. →460 nm
Product List
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Wakopak® Wakosil® 5SIL
Wakopak® Wakosil® 10SIL
Wakopak® Wakosil® 5SIL-120
Wakopak® Wakosil® 7SIL-120
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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