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Adsorptive silica gel

Wakopak® Wakosil® SIL Series

Wakopak® Wakosil® SIL Series use two types of silica gel packing agent: the high-adsorbing type, Wakosil® SIL, with a pore size of 6 nm and the low-adsorbing type, Wakosil® SIL-120, with a pore size of 12 nm. Their specific surface areas are 500 m2/g and 300 m2/g, respectively. The larger the specific surface area, the stronger the sample retention and the higher the resolution. Two adsorption quantities can be selected for more efficient analysis.

Types and Physical Properties of Packing Agents

Packing Agents Average Particle Size
(µm)
Average Pore Size
(nm)
Specific Surface Area
(m2/g)
Pore ​​Volume
(mL/g)
High-adsorbing Type Wakosil® 5SIL 5 6 500 0.75
Wakosil® 10SIL 10 6 500 0.75
Low-adsorbing Type Wakosil® 5SIL-120 5 12 300 1.0
Wakosil® 7SIL-120 7 12 300 1.0

Applications

Vitamin E homologues analysis

01060_img01.png
Column Size
Eluent
Flow Rate
Detection
Injection Volume
: 4.6 mm x 150 mm
: n-Hexane/DIPE=90/10 (v/v)
: 2.0 mL/min. at R.T.
: UV 295 nm 0.32AUFS
: 3 μL

 

Corticosteroids analysis

  • 01060_img02.png
  • Column
    Eluent
    Flow Rate
    Detection
    : Wakopak® Wakosil® 5SIL (4.6 mm x 150 mm)
    : n-Hexane/CHCl3/EtOH=7/2/1 (v/v/v)
    : 1.0 mL/min. at R.T.
    : UV 250 nm 0.04AUFS

 

Simultaneous analysis of five aflatoxins

  • 01060_img03R.png
  • Column
    Eluent
    Flow Rate
    Detection
    Injection Volume
    : Wakopak® Wakosil® 5SIL (4.6 mm x 250 mm)
    : Toluene/Ethyl Acetate/Formic Acid/CH3OH=50/5/1/2 (v/v/v/v)
    : 1.0 mL/min. at R.T.
    : FL λex 365 nm, λem 425 nm
    : 10 μL

 

Analysis of chlorophyll a and b

  • 01060_img04.png
  • Column
    Eluent
    Flow Rate
    Detection

    :Wakopak® Wakosil® 5SIL-120 (4.6 mm x 150 mm)
    n-Hexane/2-Propanol=98.5/1.5 (v/v)
    :1.0 mL/min. at 0℃
    :0→6 min. 430 nm
     6 min. →460 nm

Product List

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Wakopak® Wakosil® 5SIL

Adsorption Chromatography Column

Wakopak® Wakosil® 10SIL

Adsorption Chromatography Column

Wakopak® Wakosil® 5SIL-120

Adsorption Chromatography Column

Wakopak® Wakosil® 7SIL-120

Adsorption Chromatography Column

Product content may differ from the actual image due to minor specification changes etc.

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