For culturing neurons and neural stem cells

NS Basal Medium / NS Supplement

NS Basal Medium is a chemically-defined synthetic medium optimized for culturing nerve cells. NS Supplement is a serum-free supplement (containing retinyl acetate) specifically designed for supporting the growth of nerve cells. When mixed together, they can be used for culturing a variety of nerve cells, including rat hippocampal neurons and neural stem cells.

Primary Culture of Rat Hippocampal Neurons

Rat hippocampal neurons isolated from E19 embryos were cultured using poly-L-lysine-coated microtiter plates.
The morphology of these neurons was analyzed on day 6. We also examined the expression of the neuronal markers MAP2(a+b) and the glial marker GFAP on day 21

Day 6

00578_img01.png

< Growth Medium >
NS Basal Medium containing 2% NS Supplement and 0.5 mM L-glutamine

< Seeding Density >
13,000cells/well(96well plate)

Day 21

00578_img02.gif

Primary Culture of Rat Cortical Neurons

Primary cortical neurons isolated from E17 rat embryos were cultured using poly-L-lysine-coated microtiter plates.
The morphology of these neurons was analyzed on day 8. We also examined the expression of the neuronal markers MAP2(a+b) and the glial marker GFAP on day 14.

Day 8

00578_img03.png

< Growth Medium >
NS Basal Medium containing 2% NS Supplement and 0.5 mM L-glutamine

< Seeding Density >
60,000cells/well(96well plate)

Day 14

00578_img04.gif

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Primary Culture of Rat Hippocampal Neurons

Primary Culture of Rat Cortical Neurons

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