Insect Cell Culture Media for Vaccine Production

WakoVAC PSFM-J1

Licensed Formulation

Formulated for insect cell expansion. Designed to maximize insect cell growth, viability and productivity. WakoVAC PSFM-J1 manufactured in Wako outperforms the same formulation manufactured in competitor's site due to using quality raw materials and strict and robust raw material control.

Raw Material

All raw materials are documented and traceable. All raw materials used in WakoVAC PSFM-J1 are standardized to a certain specification, scalable, and provided from validated suppliers. WakoVAC PSFM-J1 contains fish-derived component (cGMP, virus-inactivated) and Hydorlysate. Without fish-derived component, the media can be defined animal component-free.

Industrial Applications

The intended use of WakoVAC (GMP-compliant manufactured) is for research, process development, large-scale manufacturing/commercialization. WakoVAC is scalable to commercial volumes for industrial application. Both liquid and powder are available. Flexible packaging is also available upon request. Wako provides complete workflow support from media optimization service to regulatory support at the time of IND/NDA.

Performance

WakoVAC PSJF-J1 supports a wide range of insect cells: Sf+, Hi5, Sf9, Sf21. It is designed to maximize and consistent insect cell growth, viability and sustains high productivity. Best in class performance, Easy to use, Ongoing stability.

Features

  • Serum Free
  • Contain fish-oil (cGMP) and yeast-derived hydrolysate
  • Support a wide range of insect cells: Sf+, Sf9, Sf21, Hi5 (Especially perform best with Sf+)
  • Does not need to supplement L-Glutamine
  • Assure lot-to-lot consistency
  • Optimized for small- to large-scale vaccine production
  • Compatible with a range of scales of production from research to industry
  • GMP-compliant manufacturing and materials management including raw material changes
Product Description Supplier SF ACF PF CD GMP Hydrolysate Cell Type Shelf-life Storage Size
Sf Insect FUJIFILM Irvine Scientific Yes Yes     Yes + Sf9, Sf21 1 year 2-8℃ 1L
WakoVAC PSFM-J1 FUJIFILM Wako Yes Fish-derived component Yes   Yes ++ Sf+, Hi5, Sf9, and Sf21 1 year 2-10℃ 1L

Product Information

Cat No. 160-25851 PN TBD
Form Liquid Powder
Packaging 1L 8kg and/or Customs upon requests
Shelf-life 1 year from the date of MFG 2 years from the date of MFG
Storage 2-10˚C, protect from light

Performance

Consistently high performance regardless of lot

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Protein Expression

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Retain robust cell growth, viability and target expression with Sf9

Cell Growth
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Protein Expression Testing
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M: Molecular weight size marker
Lane 1: Wako VAC PSFM-J1
Lane 2: Medium B Control
: Target protein
  • The amount of samples being used: 20μl/Lane
  • SDS-PAGE: SuperSep™ Ace 10-20% (Wako Code: 191-15031),
    200v constant voltage for 60 minutes
  • Western blotting: PVDF membrane
  • Antibody: Anti- his Tag antibody
* Data provided by the Goshima Lab from Nagoya University
Cell Viability
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Retain robust cell growth, viability and target expression with Hi5

Cell Growth
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Protein Expression Testing
00553_img14.png
M: Molecular weight size marker
Lane 1: Wako VAC PSFM-J1
Lane 2: Wako VAC PSFM-J1 + L-Glutamine
Lane 3: Medium C Control + L-Glutamine
: Target protein
  • The amount of samples being used: 20μl/Lane
  • SDS-PAGE: SuperSep™ Ace 10-20% (Wako Code: 191-15031),
    200v constant voltage for 60 minutes
  • Western blotting: PVDF membrane
  • Antibody: Anti- his Tag antibody
* Data provided by Mirai-Kougaku
Cell Viability
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Retain target expression with Sf21

Protein Expression Testing

M: Molecular weight size marker
Lane 1: Medium A (Pre-viral inoculation)
Lane 2: Medium A (Post-viral inoculation)
Lane 3: PSFM-J1 (Pre-viral inoculation)
Lane 4: PSFM-J1 (Post-viral inoculation)
: Target protein
  • The amount of samples being used: 15μl/Lane
  • SDS-PAGE: 5-20% gradient gel, 200v constant
  • Voltage for 50 minutes

* Data provided by the Goshima Lab from Nagoya University

Pharmaceutical Raw Materials

Pharmaceutical Raw Materials: CertiPro series

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Polyacrylamide gel SuperSep™ Ace

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.