Insect Cell Culture Medium: WakoVAC PSFM-J1

Licensed Formulation

Formulated for insect cell expansion. Designed to maximize insect cell growth, viability and productivity. WakoVAC PSFM-J1 manufactured in Wako outperforms the same formulation manufactured in competitor's site due to using quality raw materials and strict and robust raw material control.

Raw Material

All raw materials are documented and traceable. All raw materials used in WakoVAC PSFM-J1 are standardized to a certain specification, scalable, and provided from validated suppliers. WakoVAC PSFM-J1 contains Cod-Liver Oil (cGMP, virus-inactivated) and Hydorlysate (yeast-derived). Without Cod-Liver Oil, the media can be defined animal component-free.

Industrial Applications

The intended use of WakoVAC (GMP-compliant manufactured) is for research, process development, large-scale manufacturing/commercialization. WakoVAC is scalable to commercial volumes for industrial application. Both liquid and powder are available. Flexible packaging is also available upon request. Wako provides complete workflow support from media optimization service to regulatory support at the time of IND/NDA.

Performance

WakoVAC PSJF-J1 supports a wide range of insect cells: Sf+, Hi5, Sf9, Sf21. It is designed to maximize and consistent insect cell growth, viability and sustains high productivity. Best in class performance, Easy to use, Ongoing stability.

Features

  • Support wide range of insect cells
  • Performs best with Sf+
    • Cell Growth

    • Cell Viability


  • Cod-liver oil(cGMP) and yeast-derived hydrolysate contained
  • CoA for non-GMP and GMP available
    Cell growth, cell viability, and protein expression data can be included on CoA upon request
  • Intended use: for research and further manufacturing
Wako Cat# 160-25851 TBD
Format Liquid Powder
Package 1L 8kg and/or Customs upon request
Shelf-life 1 year from the date of MFG 2 years from the date of MFG
Storage Condition 2-10˚C, protect from light

Other Information

  • Assure lot-to-lot consistency
  • Fine protein expression as well with Sf9, Sf21 and Hi5
  • L-Glutamine does not need to be supplemented
  • Comparative Landscape
    Product Description Supplier SF ACF PF CD GMP Yeast Extract Cell Type Shelf-life Storage Size Bag Avaiability
    Sf Insect FUJIFILM Irvine Scientific Yes Yes     Yes Low Sf9, Sf21 1 year 2-8℃ 1L 5L(MTO) Custom upon request
    WakoVAC PSFM-J1 FUJIFILM Wako Yes Cod Liver Oil Yes   Yes Medium Sf+, Hi5, Sf9, and Sf21 1 year 2-10℃ 1L 10L and 20L Powder available

Culturing Sf9 Cells

Retain robust cell growth, viability and target expression with Sf9

Cell Growth
00553_img09.png
Protein Expression Testing
00553_img11.png
M: Molecular weight size marker
Lane 1: Wako VAC PSFM-J1
Lane 2: Medium B Control
: Target protein
  • The amount of samples being used: 20μl/Lane
  • SDS-PAGE: SuperSep™ Ace 10-20% (Wako Code: 191-15031),
    200v constant voltage for 60 minutes
  • Western blotting: PVDF membrane
  • Antibody: Anti- his Tag antibody
* Data provided by the Goshima Lab from Nagoya University
Cell Viability
00553_img10R.png

Culturing Hi5 Cells

Retain robust cell growth, viability and target expression with Hi5

Cell Growth
00553_img12R.png
Protein Expression Testing
00553_img14.png
M: Molecular weight size marker
Lane 1: Wako VAC PSFM-J1
Lane 2: Wako VAC PSFM-J1 + L-Glutamine
Lane 3: Medium C Control + L-Glutamine
: Target protein
  • The amount of samples being used: 20μl/Lane
  • SDS-PAGE: SuperSep™ Ace 10-20% (Wako Code: 191-15031),
    200v constant voltage for 60 minutes
  • Western blotting: PVDF membrane
  • Antibody: Anti- his Tag antibody
* Data provided by Mirai-Kougaku
Cell Viability
00553_img13R.png

Target expression data with Sf21

Retain target expression with Sf21

Protein Expression Testing

M: Molecular weight size marker
Lane 1: Medium A (Pre-viral inoculation)
Lane 2: Medium A (Post-viral inoculation)
Lane 3: PSFM-J1 (Pre-viral inoculation)
Lane 4: PSFM-J1 (Post-viral inoculation)
: Target protein
  • The amount of samples being used: 15μl/Lane
  • SDS-PAGE: 5-20% gradient gel, 200v constant
  • Voltage for 50 minutes

* Data provided by the Goshima Lab from Nagoya University

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Polyacrylamide gel SuperSep™ Ace

Product content may differ from the actual image due to minor specification changes etc.

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