Analysis for PFAS (PFOS, PFOA, PFHxS, etc.)

Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), which are polyfluoroalkyl substances (PFCs / PFAS), are difficult to decompose in the environment and have a high accumulation potential. They are subject to various international regulations, including the Stockholm Convention on Persistent Organic Pollutants (POPs)*.

Fujifilm Wako provides pretreatment columns, solvents, and analytical standards that can be used to analyze PFAS (PFOS, PFOA, and PFHxS).

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    PFOS (PerFluoroOctaneSulfonic acid)
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    PFOA (PerFluoroOctanoic acid)
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    PFHxS (PerFluoroHexaneSulfonic acid)

* POPs
Chemical substances that are highly toxic, persistent, bioaccumulative, mobile over long distances, and have adverse effects on human health or the environment are called Persistent Organic Pollutants (POPs). POPs are required to eliminate / limit, reduce emissions, and properly dispose of waste.

3 PFCs Mixture Standard Solution

Fujifilm Wako provides mixture standard solutions and internal standard solution (13C labels) for PFHxS, PFOS, and PFOA which are compounds subject to JP Water Quality Act. Both are mixture standard solutions with clear concentration value of linear PFAS.


✔ Mixture standard solutions with clear concentration value of linear PFAS!

LC/MS Chromatogram (3 PFCs Mixture Standard Solution)

Peak No. Constituent Monitoring Ion (m/z) Mode
1 PFOA 413 -
2 PFHxS 399 -
3 PFOS 499 -

Analysis conditions

Wakopak Ultra C18-2 2.1×100 mm
Delay column
Wakopak Ultra C18-3 2.0×150 mm
Column temperature
A) 0.01 mol/L CH3COONH4 in H2O, B) CH3CN
Time (min) B conc. (%)
0-2 20
2-22 20-95
22-30 95

Additive Recovery Test (PFOA, PFOS) using Presep®-C Agri (Short)


Ultrapure Water: for PFOS-PFOA Analysis Grade
Standard Solution: PFOS・PFOA mixture standard solution (PFOS (linear): 78 μg/L, PFOA (linear): 100 μg/L)
Internal Standard Solution: 13C-PFOS・13C-PFOA mixture standard solution (PFOS: 100 μg/L, PFOA: 100 μg/L)

  • Collection / Concentration Operation
    Water (500 mL) + Standard and Internal Standard Solution
    Presep®-C Agri (Flow rate 5 mL/min.)
    Washing (H2O 5 mL)
    Dry (N2 purge)
    Extraction (0.1%NH3 in CH3OH 5 mL)
    Concentration (→0.5 mL)
  • Column Conditioning
    1. 0.1%NH3 in CH3OH 4 mL
    2. CH3OH 4 mL
    3. H2O 4 mL
LC/MS/MS Conditions
  • Column: Wakopak® Ultra C18-3 2.0 mm×150 mm
    Eluent: A)10 mM CH3COONH4 in H2O, B)CH3CN
    LC/MS/MS: Prominence LC-20ADXR / LCMS 8030 plus

  • Gradient:

    Time (min) B conc. (%)
    0-1 25
    1-26 25-100
    26-30 100
    30-30.01 100-25
    30.01-35 25


  • Chromatogram
  • Recovery Result
    Monitoring Ions (m/z) Recovery Rate
    Precursor Product
    PFOS 498.8 80.0 100.9%
    PFOA 412.9 169.1 95.0%

Product List

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Solid-Phase Extraction Columns

HPLC Column

Delay Column


*A solvent that is assured to contain PFOS and PFOA below standard values ​​by "Suitability for PFOS-PFOA determination Test".

Mixture Standard Solution

Internal Standards Mixture Solution

Analytical Standard

Author: Y. Nakamura

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.


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