Analysis for PFAS (PFOS, PFOA, PFHxS, etc.)

Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), which are polyfluoroalkyl substances (PFCs / PFAS), are difficult to decompose in the environment and have a high accumulation potential. They are subject to various international regulations, including the Stockholm Convention on Persistent Organic Pollutants (POPs)*.

Fujifilm Wako provides pretreatment columns, solvents, and analytical standards that can be used to analyze PFAS (PFOS, PFOA, and PFHxS).

PFOS (PerFluoroOctaneSulfonic acid)
PFOA (PerFluoroOctanoic acid)
PFHxS (PerFluoroHexaneSulfonic acid)

* POPs
Chemical substances that are highly toxic, persistent, bioaccumulative, mobile over long distances, and have adverse effects on human health or the environment are called Persistent Organic Pollutants (POPs). POPs are required to eliminate / limit, reduce emissions, and properly dispose of waste.

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3 PFCs Mixture Standard Solution
Additive Recovery Test (PFOA, PFOS) using Presep^®-C Agri (Short)
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3 PFCs Mixture Standard Solution

Fujifilm Wako provides mixture standard solutions and internal standard solution (¹³C labels) for PFHxS, PFOS, and PFOA which are compounds subject to JP Water Quality Act. Both are mixture standard solutions with clear concentration value of linear PFAS.

Features

✔ Mixture standard solutions with clear concentration value of linear PFAS!

LC/MS Chromatogram (3 PFCs Mixture Standard Solution)

Peak No.	Constituent	Monitoring Ion (m/z)	Mode
1	PFOA	413	-
2	PFHxS	399	-
3	PFOS	499	-

Analysis conditions

[HPLC]

Column: ： Wakopak Ultra C18-2　2.1×100 mm

Delay column: ： Wakopak Ultra C18-3　2.0×150 mm

Column temperature: ： 40℃

Eluent: ： A) 0.01 mol/L CH₃COONH₄ in H₂O, B) CH₃CN

Gradient

：

Time (min)	B conc. (%)
0-2	20
2-22	20-95
22-30	95

[MS]

Ionization: ： ESI

Mode: ： SIM

Additive Recovery Test (PFOA, PFOS) using Presep^®-C Agri (Short)

Sample

Ultrapure Water: for PFOS-PFOA Analysis Grade
Standard Solution: PFOS・PFOA mixture standard solution (PFOS (linear): 78 μg/L, PFOA (linear): 100 μg/L)
Internal Standard Solution: ¹³C-PFOS・¹³C-PFOA mixture standard solution (PFOS: 100 μg/L, PFOA: 100 μg/L)

Collection / Concentration Operation

Water (500 mL) + Standard and Internal Standard Solution

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Presep^®-C Agri (Flow rate 5 mL/min.)

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Washing (H₂O 5 mL)

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Dry (N₂ purge)

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Extraction (0.1%NH₃ in CH₃OH 5 mL)

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Concentration (→0.5 mL)

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LC/MS/MS

Column Conditioning
1. 0.1%NH₃ in CH₃OH 4 mL
2. CH₃OH 4 mL
3. H₂O 4 mL

LC/MS/MS Conditions

Column: Wakopak^® Ultra C18-3 2.0 mm×150 mm
Eluent: A)10 mM CH₃COONH₄ in H₂O, B)CH₃CN
LC/MS/MS: Prominence LC-20ADXR / LCMS 8030 plus
Gradient:

Time (min) B conc. (%)

0-1 25

1-26 25-100

26-30 100

30-30.01 100-25

30.01-35 25

Time (min)	B conc. (%)
0-1	25
1-26	25-100
26-30	100
30-30.01	100-25
30.01-35	25

Result

Chromatogram
Recovery Result

Monitoring Ions (m/z) Recovery Rate

Precursor Product

PFOS 498.8 80.0 100.9%

PFOA 412.9 169.1 95.0%

	Monitoring Ions (m/z)	Recovery Rate
Precursor	Product
PFOS	498.8	80.0	100.9%
PFOA	412.9	169.1	95.0%

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Solid-Phase Extraction Columns

HPLC Column

Delay Column

Solvents

*A solvent that is assured to contain PFOS and PFOA below standard values by "Suitability for PFOS-PFOA determination Test".

Mixture Standard Solution

Internal Standards Mixture Solution

Analytical Standard

Author: Y. Nakamura

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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