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for Alga Growth Inhibition Tests

OECD Medium, Stock Solution for Alga Growth Inhibition Tests

OECD Culture Medium, Stock Solution I, II, III & IV are intended for preparation of OECD culture medium as described in OECD Guidelines for Testing of Chemicals, specifically with respect to Guideline 201: Algal Growth Inhibition Test.
The Algal Growth Inhibition Test uses WET (Whole Effluent Toxicity) test method which measures the effect of wastewater on specific test organisms' ability to survive, grow and reproduce.

Features

  • No weighing and dissolution necessary
  • Mycoplasma tested
  • Endotoxin tested
  • Sterilized by 0.2 μm filtration
  • Based on OECD Test Guidelines 201

Preparation

Preparation of OECD Culture Medium based on OECD Test Guidelines 201

For preparation of 1L of OECD Culture Medium based on OECD Test Guidelines 201, take the OECD Medium, Stock solution I - IV as shown below, mix together and dilute with ion-exchanged water, etc. to make the 1L solution.

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Components

Components of OECD Stock Solution I - IV

Cat# Pkg. size Components Concentration
(Stock Solution: mg/L)
Concentration
(after dilution: mg/L)
158-03315 500mL OECD Medium, Stock Solution Ⅰ(x100) x100 x1
NH4Cl 1,500.00 15
MgCl2·6H2O 1,200.00 12
CaCl2·2H2O 1,800.00 18
MgSO4·7H2O 1,500.00 15
KH2PO4 160 1.6
153-03321 50mL OECD Medium, Stock Solution Ⅱ(x1,000) x1,000 x1
FeCl3·6H2O 64 0.064
EDTA-Na2·2H2O 100 0.1
150-03331 50mL OECD Medium, Stock Solution Ⅲ(x1,000) x1,000 x1
H3BO3 185 0.185
MnCl2·4H2O 415 0.415
ZnCl2 3 0.003
CoCl2·6H2O 1.5 0.0015
CuCl2·2H2O 0.01 0.00001
Na2MoO4·2H2O 7 0.007
157-03341 50mL OECD Medium, Stock Solution Ⅳ(x1,000) x1,000 x1
NaHCO3 50,000.00 50

Data

Example Analysis

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Author: A. Samoveanu

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