ウエスタンブロッティング、免疫組織染色、ELISAにおける発色検出

Jackson社 アルカリホスファターゼ (AP)、ペルオキシダーゼ (HRP) 標識体

発色基質を利用した発色検出法は、組織を染色する免疫組織化学 (IHC) からウエスタンブロッティングまで、様々な免疫染色において使用されています。

アルカリホスファターゼ (AP) やペルオキシダーゼ (HRP) など、レポーター酵素を標識した抗体をあらかじめインキュベートしたメンブレンや組織に、発色基質を添加し、酵素反応によって発色基質を着色沈殿物に転換させます。ウエスタンブロット上で生成した着色沈殿物は、特別な機器を必要とせずに、着色バンドとして目視で確認できます。組織上の着色沈殿物は免疫組織化学 (IHC) で使用される通常の光学顕微鏡で見ることができます。

発色検出法は、最適化や高バックグラウンドを改善するための追加の染色が必要な場合もありますが、非常に簡便で安価な検出法です。

Jackson社では、発色検出法に使用するアルカリホスファターゼ (AP) とペルオキシダーゼ (HRP) の標識体を多数ラインアップしています。

間接発色検出法:
A:レポーター酵素標識二次抗体が、抗原と結合した一次抗体を検出
B:抗原-抗体複合体に発色基質を添加
C:二次抗体に標識されたレポーター酵素が触媒する化学反応によって、発色基質が目視可能な着色沈殿物に転換

アプリケーション

ウエスタンブロッティング

還元しSDS変性させたマウスIgGをSDS-PAGEで重鎖 (50kDa) と軽鎖 (25kDa) に分離し、ペルオキシダーゼ標識-抗マウスIgG(H+L),ヤギ 抗体と発色基質TMBを用いてウエスタンブロット上で可視化した。

ドットブロット

ニトロセルロース膜の各列に100ngのマウス total IgG および各サブクラスのIgGをスポットし、5%(w/v)BSA PBST溶液でブロッキング処理した後、ペルオキシダーゼ標識-抗マウスIgG サブクラス特異的,ヤギ抗体と反応させ、TMBM基質 (Moss, Inc.) で検出した。

免疫組織化学 (IHC)

抗GFP一次抗体をBiotin-SP標識抗ウサギIgG(H+L),ロバ二次抗体とHRP (ペルオキシダーゼ) 標識ストレプトアビジンを用いて免疫組織染色を行った。
A:移植後4週間では、生体内試料中、GFPのシグナルの検出はできなかった。
B:ポジティブコントロール中のGFP発現細胞を茶色で染色した。発色基質:DAB(Envision kit; DakoCytomation, Glostrup, Denmark)を用いて室温で10分間反応。核はヘマトキシリンで対比染色した。

使用抗体

Biotin-SP (long spacer) AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot) (メーカーコード:711-065-152)
Peroxidase Streptavidin (メーカーコード:016-030-084)

ターゲット

Anti-Alpaca (アルパカ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Goat Anti-Alpaca IgG (H+L) 128-055-003 128-035-003
Product Information Product Information
Goat Anti-Alpaca IgG (H+L)
(min X Bov,Hu,Ms,Rb,Rat Sr Prot)
128-055-160 128-035-160
Product Information Product Information
Goat Anti-Alpaca IgG, subclasses 2+3 specific
(min X Bov,Hu,Ms,Rb,Rat Sr Prot)
128-055-229 128-035-229
Product Information Product Information
Goat Anti-Alpaca IgG, VHH domain (min X Bov Sr Prot) 128-055-232 128-035-232
Product Information Product Information
Goat Anti-Alpaca IgG, VHH domain
(min X Bov,Hu,Ms,Rb,Rat Sr Prot)
128-055-230 128-035-230
Product Information Product Information

Anti-Bovine (ウシ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Goat Anti-Bovine IgG (H+L) 101-055-003 101-035-003
Product Information Product Information
F(ab')2 Fragment Goat Anti-Bovine IgG (H+L) 101-056-003 101-036-003
Product Information Product Information
Goat Anti-Bovine IgG (H+L)
(min X Ar Hms,Hu,Ms,Rat Sr Prot)
101-055-165 101-035-165
Product Information Product Information
Goat Anti-Bovine IgG, Fc Fragment Specific 101-035-008
Product Information
Rabbit Anti-Bovine IgG (H+L) 301-055-003 301-035-003
Product Information Product Information

Anti-Cat (ネコ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Goat Anti-Cat IgG (H+L) 102-055-003 102-035-003
Product Information Product Information
Goat Anti-Cat IgG, F(ab')2 Fragment Specific 102-055-006 102-035-006
Product Information Product Information
Goat Anti-Cat IgG, Fc Fragment Specific 102-055-008 102-035-008
Product Information Product Information

Anti-Chicken (ニワトリ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Donkey Anti-Chicken IgY (IgG) (H+L)
(min X Bov,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
703-055-155 703-035-155
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Chicken IgY (IgG) (H+L)
(min X Bov,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
703-056-155 703-036-155
Product Information Product Information
Goat Anti-Chicken IgY (IgG) (H+L)
(min X Bov,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
103-055-155 103-035-155
Product Information Product Information
Rabbit Anti-Chicken IgY (IgG) (H+L) 303-055-003 303-035-003
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Chicken IgY (IgG) (H+L) 303-056-003 303-036-003
Product Information Product Information
Rabbit Anti-Chicken IgY (IgG), F(ab')2 Fragment Specific 303-055-006 303-035-006
Product Information Product Information
Rabbit Anti-Chicken IgY (IgG), Fc Fragment Specific 303-055-008 303-035-008
Product Information Product Information

Anti-Dog (イヌ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Rabbit Anti-Dog IgG (H+L) 304-055-003 304-035-003
Product Information Product Information
Rabbit Anti-Dog IgG, Fc Fragment Specific 304-055-008 304-035-008
Product Information Product Information

Anti-Goat (ヤギ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Bovine Anti-Goat IgG (H+L)
(min X Bov,Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
805-055-180 805-035-180
Product Information Product Information
Donkey Anti-Goat IgG (H+L) 705-055-003 705-035-003
Product Information Product Information
Donkey Anti-Goat IgG (H+L)
(min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
705-055-147 705-035-147
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)
(min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
705-056-147 705-036-147
Product Information Product Information
Mouse Anti-Goat IgG (H+L)
(min X Hu,Ms,Rb Sr Prot)
205-055-108 205-035-108
Product Information Product Information
IgG Fraction Monoclonal Mouse Anti-Goat IgG, Light Chain Specific
(min X Hrs,Hu,Ms,Rb,Rat Ig)
205-052-176 205-032-176
Product Information Product Information
Rabbit Anti-Goat IgG (H+L) 305-055-003 305-035-003
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Goat IgG (H+L) 305-056-003 305-036-003
Product Information Product Information
Rabbit Anti-Goat IgG (H+L) (min X Hu Sr Prot) 305-055-045 305-035-045
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Goat IgG (H+L)
(min X Hu Sr Prot)
305-056-045 305-036-045
Product Information Product Information
Rabbit Anti-Goat IgG, F(ab')2 Fragment Specific 305-055-006 305-035-006
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Goat IgG, F(ab')2 Fragment Specific 305-056-006 305-036-006
Product Information Product Information
Rabbit Anti-Goat IgG, F(ab')2 Fragment Specific
(min X Hu Sr Prot)
305-055-047 305-035-047
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Goat IgG, F(ab')2 Fragment Specific
(min X Hu Sr Prot)
305-056-047 305-036-047
Product Information Product Information
Rabbit Anti-Goat IgG, Fc Fragment Specific 305-055-008 305-035-008
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Goat IgG, Fc Fragment Specific 305-056-008 305-036-008
Product Information Product Information
Rabbit Anti-Goat IgG, Fc Fragment Specific
(min X Hu Sr Prot)
305-055-046 305-035-046
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Goat IgG, Fc Fragment Specific
(min X Hu Sr Prot)
305-056-046 305-036-046
Product Information Product Information

Anti-Guinea Pig (モルモット)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Donkey Anti-Guinea Pig IgG (H+L)
(min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
706-055-148 706-035-148
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Guinea Pig IgG (H+L)
(min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
706-056-148 706-036-148
Product Information Product Information
Goat Anti-Guinea Pig IgG (H+L) 106-055-003 106-035-003
Product Information Product Information
F(ab')2 Fragment Goat Anti-Guinea Pig IgG (H+L) 106-056-003 106-036-003
Product Information Product Information
Goat Anti-Guinea Pig IgG, F(ab')2 Fragment Specific 106-055-006 106-035-006
Product Information Product Information
Goat Anti-Guinea Pig IgG, Fc Fragment Specific 106-055-008 106-035-008
Product Information Product Information

Anti-Armenian Hamster (アルメニアンハムスター)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Goat Anti-Armenian Hamster IgG (H+L) (min X Bov Sr Prot) 127-055-099 127-035-099
Product Information Product Information
Goat Anti-Armenian Hamster IgG (H+L)
(min X Bov,Hu,Ms,Rb,Rat Sr Prot)
127-055-160 127-035-160
Product Information Product Information

Anti-Syrian Hamster (シリアンハムスター)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Goat Anti-Syrian Hamster IgG (H+L)
(min X Bov,Hrs,Hu,Ms,Rb,Rat Sr Prot)
107-055-142 107-035-142
Product Information Product Information
F(ab')2 Fragment Goat Anti-Syrian Hamster IgG (H+L)
(min X Bov,Hrs,Hu,Ms,Rb,Rat Sr Prot)
107-056-142 107-036-142
Product Information Product Information
Rabbit Anti-Syrian Hamster IgG (H+L) 307-055-003 307-035-003
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Syrian Hamster IgG (H+L) 307-056-003 307-036-003
Product Information Product Information

Anti-Horse (ウマ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Goat Anti-Horse IgG (H+L) 108-055-003 108-035-003
Product Information Product Information
F(ab')2 Fragment Goat Anti-Horse IgG (H+L) 108-056-003 108-036-003
Product Information Product Information
Goat Anti-Horse IgG, Fc Fragment Specific 108-055-008 108-035-008
Product Information Product Information
Rabbit Anti-Horse IgG (H+L) 308-055-003 308-035-003
Product Information Product Information
Rabbit Anti-Horse IgG, Fc Fragment Specific 308-035-008
Product Information

Anti-Human (ヒト)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Alpaca Anti-Human IgG (H+L)
(min X Bov,Ms,Rb Sr Prot)
609-055-213 609-035-213
Product Information Product Information
Donkey Anti-Human IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Ms,Rb,Rat,Shp Sr Prot)
709-055-149 709-035-149
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Human IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Ms,Rb,Rat,Shp Sr Prot)
709-056-149 709-036-149
Product Information Product Information
Donkey Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Hrs,Ms Sr Prot)
709-055-098 709-035-098
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Hrs,Ms Sr Prot)
709-056-098 709-036-098
Product Information Product Information
Donkey Anti-Human IgM, Fc Fragment Specific
(min X Bov,Hrs Sr Prot)
709-055-073 709-035-073
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Human IgM, Fc Fragment Specific
(min X Bov,Hrs Sr Prot)
709-056-073 709-036-073
Product Information Product Information
Goat Anti-Human IgG (H+L) 109-055-003 109-035-003
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgG (H+L) 109-056-003 109-036-003
Product Information Product Information
Goat Anti-Human IgG (H+L) (min X Bov,Hrs,Ms Sr Prot) 109-055-088 109-035-088
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgG (H+L)
(min X Bov,Hrs,Ms Sr Prot)
109-056-088 109-036-088
Product Information Product Information
Goat Anti-Human IgG, F(ab')2 Fragment Specific 109-055-006 109-035-006
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgG, F(ab')2 Fragment Specific 109-056-006 109-036-006
Product Information Product Information
Goat Anti-Human IgG, F(ab')2 Fragment Specific
(min X Bov,Hrs,Ms Sr Prot)
109-055-097 109-035-097
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgG, F(ab')2 Fragment Specific
(min X Bov,Hrs,Ms Sr Prot)
109-056-097 109-036-097
Product Information Product Information
Goat Anti-Human IgG, Fcγ Fragment Specific 109-055-008 109-035-008
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgG, Fcγ Fragment Specific 109-056-008 109-036-008
Product Information Product Information
Goat Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Hrs,Ms Sr Prot)
109-055-098 109-035-098
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Hrs,Ms Sr Prot)
109-056-098 109-036-098
Product Information Product Information
Goat Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Ms Sr Prot)
109-055-190 109-035-190
Product Information Product Information
Goat Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Ms,Rb Sr Prot)
109-055-170 109-035-170
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Ms,Rb Sr Prot)
109-056-170 109-036-170
Product Information Product Information
Goat Anti-Human IgG + IgM (H+L) 109-055-044 109-035-044
Product Information Product Information
Goat Anti-Human IgG + IgM (H+L)
(min X Bov Sr Prot)
109-055-127 109-035-127
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgG + IgM (H+L)
(min X Bov Sr Prot)
109-056-127 109-036-127
Product Information Product Information
Goat Anti-Human IgM, Fc Fragment Specific 109-055-043 109-035-043
Product Information Product Information
Goat Anti-Human IgM, Fc Fragment Specific
(min X Bov Sr Prot)
109-055-129 109-035-129
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgM, Fc Fragment Specific
(min X Bov Sr Prot)
109-056-129 109-036-129
Product Information Product Information
Goat Anti-Human IgA + IgG + IgM (H+L) 109-055-064 109-035-064
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L) 109-056-064 109-036-064
Product Information Product Information
Goat Anti-Human Serum IgA, α Chain Specific 109-055-011 109-035-011
Product Information Product Information
F(ab')2 Fragment Goat Anti-Human Serum IgA, α Chain Specific 109-056-011 109-036-011
Product Information Product Information
Mouse Anti-Human IgG (H+L)
(min X Bov,Hrs,Ms Sr Prot)
209-055-088 209-035-088
Product Information Product Information
Mouse Anti-Human IgG (H+L)
(min X Ms Sr Prot)
209-055-082 209-035-082
Product Information Product Information
Mouse Anti-Human IgG, F(ab')2 Fragment Specific
(min X Bov,Hrs,Ms Sr Prot)
209-055-097 209-035-097
Product Information Product Information
Mouse Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Hrs,Ms Sr Prot)
209-055-098 209-035-098
Product Information Product Information
Rabbit Anti-Human IgG (H+L) 309-055-003 309-035-003
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Human IgG (H+L) 309-056-003 309-036-003
Product Information Product Information
Rabbit Anti-Human IgG (H+L) (min X Ms Sr Prot) 309-055-082 309-035-082
Product Information Product Information
Rabbit Anti-Human IgG, F(ab')2 Fragment Specific 309-055-006 309-035-006
Product Information Product Information
Rabbit Anti-Human IgG, Fcγ Fragment Specific 309-055-008 309-035-008
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Human IgG, Fcγ Fragment Specific 309-056-008 309-036-008
Product Information Product Information
Rabbit Anti-Human IgG + IgM (H+L)
(min X Ms Sr Prot)
309-055-107 309-035-107
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Human IgM, Fc Fragment Specific 309-056-043 309-036-043
Product Information Product Information
Rabbit Anti-Human IgM, Fc Fragment Specific
(min X Ms Sr Prot)
309-055-095 309-035-095
Product Information Product Information
Rabbit Anti-Human IgA+IgG+IgM (H+L) 309-055-064 309-035-064
Product Information Product Information
Rabbit Anti-Human Serum IgA, α Chain Specific 309-055-011 309-035-011
Product Information Product Information

Anti-Mouse (マウス)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Alpaca Anti-Mouse IgG (H+L) (min X Bov,Hu,Rb Sr Prot) 615-055-214 615-035-214
Product Information Product Information
Donkey Anti-Mouse IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot)
715-055-151 715-035-151
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot)
715-056-151 715-036-151
Product Information Product Information
Donkey Anti-Mouse IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
715-055-150 715-035-150
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
715-056-150 715-036-150
Product Information Product Information
Donkey Anti-Mouse IgM, µ Chain Specific 715-055-020 715-035-020
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Mouse IgM, µ Chain Specific 715-056-020 715-036-020
Product Information Product Information
Donkey Anti-Mouse IgM, µ Chain Specific
(min X Hu,Bov,Hrs,Rat Sr Prot)
715-055-140 715-035-140
Product Information Product Information
Goat Anti-Mouse IgG (H+L) 115-055-003 115-035-003
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) 115-056-003 115-036-003
Product Information Product Information
Goat Anti-Mouse IgG (H+L)
(min X Hu,Bov,Hrs Sr Prot)
115-055-062 115-035-062
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)
(min X Hu,Bov,Hrs Sr Prot))
115-056-062 115-036-062
Product Information Product Information
Goat Anti-Mouse IgG (H+L)
(min X Hu,Bov,Hrs,Rb,Rat Sr Prot)
115-055-166 115-035-166
Product Information Product Information
Goat Anti-Mouse IgG (H+L)
(min X Hu,Bov,Hrs,Rb,Sw Sr Prot)
115-055-146 115-035-146
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)
(min X Hu,Bov,Hrs,Rb,Sw Sr Prot)
115-056-146 115-036-146
Product Information Product Information
Goat Anti-Mouse IgG, Light Chain Specific
(min X Bov,Gt,Hrs,Hu,Rb,Rat,Shp Ig)
115-055-174 115-035-174
Product Information Product Information
Goat Anti-Mouse IgG, F(ab')2 Fragment Specific 115-055-006 115-035-006
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgG, F(ab')2 Fragment Specific 115-056-006 115-036-006
Product Information Product Information
Goat Anti-Mouse IgG, F(ab')2 Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
115-055-072 115-035-072
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgG, F(ab')2 Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
115-056-072 115-036-072
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ Fragment Specific 115-055-008 115-035-008
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgG, Fcγ Fragment Specific 115-056-008 115-036-008
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
115-055-071 115-035-071
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgG, Fcγ Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
115-056-071 115-036-071
Product Information Product Information
Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ Fragment Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-164 115-035-164
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-205 115-035-205
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-206 115-035-206
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-207 115-035-207
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-208 115-035-208
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific
(min X Hu,Bov,Rb Sr Prot)
115-055-209 115-035-209
Product Information Product Information
Goat Anti-Mouse IgG + IgM (H+L) 115-055-044 115-035-044
Product Information Product Information
Goat Anti-Mouse IgG + IgM (H+L)
(min X Hu,Bov,Hrs Sr Prot)
115-055-068 115-035-068
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgG + IgM (H+L)
(min X Hu,Bov,Hrs Sr Prot)
115-056-068 115-036-068
Product Information Product Information
Goat Anti-Mouse IgM, µ Chain Specific 115-055-020 115-035-020
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgM, µ Chain Specific 115-056-020 115-036-020
Product Information Product Information
Goat Anti-Mouse IgM, µ Chain Specific
(min X Hu,Bov,Hrs Sr Prot)
115-055-075 115-035-075
Product Information Product Information
F(ab')2 Fragment Goat Anti-Mouse IgM, µ Chain Specific
(min X Hu,Bov,Hrs Sr Prot)
115-056-075 115-036-075
Product Information Product Information
Rabbit Anti-Mouse IgG (H+L) 315-055-003 315-035-003
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Mouse IgG (H+L) 315-056-003 315-036-003
Product Information Product Information
Rabbit Anti-Mouse IgG (H+L) (min X Hu Sr Prot) 315-055-045 315-035-045
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Mouse IgG (H+L)
(min X Hu Sr Prot)
315-056-045 315-036-045
Product Information Product Information
Rabbit Anti-Mouse IgG, F(ab')2 Fragment Specific 315-055-006 315-035-006
Product Information Product Information
Rabbit Anti-Mouse IgG, F(ab')2 Fragment Specific
(min X Hu Sr Prot)
315-055-047 315-035-047
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Mouse IgG, F(ab')2 Fragment Specific
(min X Hu Sr Prot)
315-056-047 315-036-047
Product Information Product Information
Rabbit Anti-Mouse IgG, Fcγ Fragment Specific 315-055-008 315-035-008
Product Information Product Information
Rabbit Anti-Mouse IgG, Fcγ Fragment Specific
(min X Hu Sr Prot)
315-055-046 315-035-046
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Mouse IgG, Fcγ Fragment Specific
(min X Hu Sr Prot)
315-056-046 315-036-046
Product Information Product Information
Rabbit Anti-Mouse IgG + IgM (H+L) 315-055-044 315-035-044
Product Information Product Information
Rabbit Anti-Mouse IgG + IgM (H+L)
(min X Hu Sr Prot)
315-055-048 315-035-048
Product Information Product Information
Rabbit Anti-Mouse IgM, µ Chain Specific 315-055-020 315-035-020
Product Information Product Information
Rabbit Anti-Mouse IgM, µ Chain Specific
(min X Hu Sr Prot)
315-055-049 315-035-049
Product Information Product Information
Rat Anti-Mouse IgG (H+L)
(min X Hu,Bov,Hrs,Rb,Rat Sr Prot)
415-055-166 415-035-166
Product Information Product Information
Sheep Anti-Mouse IgG (H+L) 515-055-003 515-035-003
Product Information Product Information
Sheep Anti-Mouse IgG (H+L)
(min X Hu,Bov,Hrs Sr Prot)
515-055-062 515-035-062
Product Information Product Information
Sheep Anti-Mouse IgG, F(ab')2 Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
515-055-072 515-035-072
Product Information Product Information
Sheep Anti-Mouse IgG, Fcγ Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
515-055-071 515-035-071
Product Information Product Information

Anti-Rabbit (ウサギ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Alpaca Anti-Rabbit IgG (H+L)
(min X Bov,Hu,Ms Sr Prot)
611-055-215 611-035-215
Product Information Product Information
Donkey Anti-Rabbit IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)
711-055-152 711-035-152
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)
711-056-152 711-036-152
Product Information Product Information
Goat Anti-Rabbit IgG (H+L) 111-055-003 111-035-003
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) 111-056-003 111-036-003
Product Information Product Information
Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) 111-055-045 111-035-045
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)
(min X Hu Sr Prot)
111-056-045 111-036-045
Product Information Product Information
Goat Anti-Rabbit IgG (H+L)
(min X Hu,Ms,Rat Sr Prot)
111-055-144 111-035-144
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)
(min X Hu,Ms,Rat Sr Prot)
111-056-144 111-036-144
Product Information Product Information
Goat Anti-Rabbit IgG, F(ab')2 Fragment Specific 111-055-006 111-035-006
Product Information Product Information
Goat Anti-Rabbit IgG, F(ab')2 Fragment Specific
(min X Hu Sr Prot)
111-055-047 111-035-047
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rabbit IgG, F(ab')2 Fragment Specific (min X Hu Sr Prot) 111-056-047 111-036-047
Product Information Product Information
Goat Anti-Rabbit IgG, Fc Fragment Specific 111-055-008 111-035-008
Product Information Product Information
Goat Anti-Rabbit IgG, Fc Fragment Specific (min X Hu Sr Prot) 111-055-046 111-035-046
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rabbit IgG, Fc Fragment Specific (min X Hu Sr Prot) 111-056-046 111-036-046
Product Information Product Information
Mouse Anti-Rabbit IgG (H+L)
(min X Hu,Gt,Ms,Shp Sr Prot)
211-055-109 211-035-109
Product Information Product Information
IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific
(min X Bov,Gt,Arm Hms,Hrs,Hu,Ms,Rat,Shp Ig)
211-052-171 211-032-171
Product Information Product Information

Anti-Rat (ラット)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Donkey Anti-Rat IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot)
712-055-153 712-035-153
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Rat IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot)
712-056-153 712-036-153
Product Information Product Information
Donkey Anti-Rat IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
712-055-150 712-035-150
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Rat IgG (H+L)
(min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
712-056-150 712-036-150
Product Information Product Information
Goat Anti-Rat IgG (H+L) 112-055-003 112-035-003
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rat IgG (H+L) 112-056-003 112-036-003
Product Information Product Information
Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs Sr Prot) 112-055-062 112-035-062
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rat IgG (H+L)
(min X Hu,Bov,Hrs Sr Prot)
112-056-062 112-036-062
Product Information Product Information
Goat Anti-Rat IgG (H+L)
(min X Hu,Bov,Hrs,Ms,Rb Sr Prot)
112-055-167 112-035-167
Product Information Product Information
Goat Anti-Rat IgG (H+L)
(min X Hu,Bov,Hrs,Rb Sr Prot)
112-055-143 112-035-143
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rat IgG (H+L)
(min X Hu,Bov,Hrs,Rb Sr Prot)
112-056-143 112-036-143
Product Information Product Information
Goat Anti-Rat IgG, Light Chain Specific
(min X Bov,Gt,Hrs,Hu,Ms,Rb,Shp Ig)
112-055-175 112-035-175
Product Information Product Information
Goat Anti-Rat IgG, F(ab')2 Fragment Specific 112-055-006 112-035-006
Product Information Product Information
Goat Anti-Rat IgG, F(ab')2 Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
112-055-072 112-035-072
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rat IgG, F(ab')2 Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
112-056-072 112-036-072
Product Information Product Information
Goat Anti-Rat IgG, Fcγ Fragment Specific 112-055-008 112-035-008
Product Information Product Information
Goat Anti-Rat IgG, Fcγ Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
112-055-071 112-035-071
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rat IgG, Fcγ Fragment Specific
(min X Hu,Bov,Hrs Sr Prot)
112-056-071 112-036-071
Product Information Product Information
Goat Anti-Rat IgG + IgM (H+L) 112-055-044 112-035-044
Product Information Product Information
Goat Anti-Rat IgG + IgM (H+L)
(min X Hu,Bov,Hrs Sr Prot)
112-055-068 112-035-068
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rat IgG + IgM (H+L)
(min X Hu,Bov,Hrs Sr Prot)
112-056-068 112-036-068
Product Information Product Information
Goat Anti-Rat IgM, µ Chain Specific 112-055-020 112-035-020
Product Information Product Information
Goat Anti-Rat IgM, µ Chain Specific
(min X Hu,Bov,Hrs Sr Prot)
112-055-075 112-035-075
Product Information Product Information
F(ab')2 Fragment Goat Anti-Rat IgM, µ Chain Specific
(min X Hu,Bov,Hrs Sr Prot)
112-056-075 112-036-075
Product Information Product Information
Mouse Anti-Rat IgG (H+L)
(min X Ms Sr Prot)
212-055-082 212-035-082
Product Information Product Information
Mouse Anti-Rat IgG (H+L)
(min X Hu,Bov,Hrs,Ms,Gt,Rb Sr Prot)
212-055-168 212-035-168
Product Information Product Information
F(ab')2 Fragment Mouse Anti-Rat IgG (H+L)
(min X Hu,Bov,Hrs,Ms,Gt,Rb Sr Prot)
212-056-168 212-036-168
Product Information Product Information
Mouse Anti-Rat IgG, F(ab')2 Fragment Specific
(min X Hu,Bov,Hrs,Ms Sr Prot)
212-055-106 212-035-106
Product Information Product Information
Mouse Anti-Rat IgG, Fcγ Fragment Specific
(min X Hu,Bov,Hrs,Ms Sr Prot)
212-055-104 212-035-104
Product Information Product Information
Rabbit Anti-Rat IgG (H+L) 312-055-003 312-035-003
Product Information Product Information
Rabbit Anti-Rat IgG (H+L) (min X Hu Sr Prot) 312-055-045 312-035-045
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Rat IgG (H+L)
(min X Hu Sr Prot)
312-056-045 312-036-045
Product Information Product Information
Rabbit Anti-Rat IgG, F(ab')2 Fragment Specific
(min X Hu Sr Prot)
312-055-047 312-035-047
Product Information Product Information
Rabbit Anti-Rat IgG, Fcγ Fragment Specific
(min X Hu Sr Prot)
312-055-046 312-035-046
Product Information Product Information
Rabbit Anti-Rat IgG + IgM (H+L) 312-055-044 312-035-044
Product Information Product Information
Rabbit Anti-Rat IgG + IgM (H+L)
(min X Hu Sr Prot)
312-055-048 312-035-048
Product Information Product Information
Rabbit Anti-Rat IgM, µ Chain Specific 312-055-020 312-035-020
Product Information Product Information
Rabbit Anti-Rat IgM, µ Chain Specific
(min X Hu Sr Prot)
312-055-049 312-035-049
Product Information Product Information

Anti-Sheep (ヒツジ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Donkey Anti-Sheep IgG (H+L) 713-055-003 713-035-003
Product Information Product Information
Donkey Anti-Sheep IgG (H+L)
(min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
713-055-147 713-035-147
Product Information Product Information
F(ab')2 Fragment Donkey Anti-Sheep IgG (H+L)
(min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
713-056-147 713-036-147
Product Information Product Information
IgG Fraction Monoclonal Mouse Anti-Sheep IgG, Light Chain Specific
(min X Bov,Hrs,Hu,Ms,Rb,Rat Ig)
213-052-177 213-032-177
Product Information Product Information
Rabbit Anti-Sheep IgG (H+L) 313-055-003 313-035-003
Product Information Product Information
F(ab')2 Fragment Rabbit Anti-Sheep IgG (H+L) 313-056-003 313-036-003
Product Information Product Information
Rabbit Anti-Sheep IgG (H+L) (min X Hu Sr Prot) 313-055-045 313-035-045
Product Information Product Information
Rabbit Anti-Sheep IgG, F(ab')2 Fragment Specific
(min X Hu Sr Prot)
313-055-047 313-035-047
Product Information Product Information
Rabbit Anti-Sheep IgG, Fc Fragment Specific
(min X Hu Sr Prot)
313-055-046 313-035-046
Product Information Product Information

Anti-Swine (ブタ)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Goat Anti-Swine IgG (H+L) 114-055-003 114-035-003
Product Information Product Information

Anti-Biotin (ビオチン)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
IgG Fraction Monoclonal Mouse Anti-Biotin 200-052-211 200-032-211
Product Information Product Information

Anti-Digoxin (ジゴキシン)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
IgG Fraction Monoclonal Mouse Anti-Digoxin 200-052-156 200-032-156
Product Information Product Information

Anti-Fluorescein (フルオレセイン、FITC)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
IgG Fraction Monoclonal Mouse Anti-Fluorescein (FITC) 200-052-037 200-032-037
Product Information Product Information

Anti-Horseradish Peroxidase (ペルオキシダーゼ、HRP)

抗体名 Alkaline Phosphatase
Goat Anti-Horseradish Peroxidase 123-055-021
Product Information
Rabbit Anti-Horseradish Peroxidase 323-055-021
Product Information

Anti-Lactoferrin (ラクトフェリン)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Rabbit Anti-Human Lactoferrin 309-055-015 309-035-015
Product Information Product Information

Albumin (アルブミン)

抗体名 Horseradish Peroxidase
Human Albumin 009-030-051
Product Information

Streptavidin (ストレプトアビジン)

抗体名 Alkaline Phosphatase Horseradish Peroxidase
Streptavidin 016-050-084 016-030-084
Product Information Product Information

Transferrin (トランスフェリン)

抗体名 Horseradish Peroxidase
Human Transferrin 009-030-050
Product Information
Mouse Transferrrin 015-030-050
Product Information
Rat Transferrin 012-030-050
Product Information

IgG (免疫グロブリン)

抗体名 Horseradish Peroxidase
Bovine IgG, whole molecule 001-030-003
Product Information
Chicken IgY (IgG), whole molecule 003-030-003
Product Information
Donkey IgG, whole molecule 017-030-003
Product Information
Donkey IgG, F(ab')2 fragment 017-030-006
Product Information
Goat IgG, whole molecule 005-030-003
Product Information
Goat IgG, F(ab')2 fragment 005-030-006
Product Information
Syrian Hamster IgG, whole molecule 007-030-003
Product Information
Human IgG, whole molecule 009-030-003
Product Information
Human IgG, F(ab')2 fragment 009-030-006
Product Information
Human IgG, Fab fragment 009-030-007
Product Information
Human IgG, Fc fragment 009-030-008
Product Information
Human IgM (myeloma), whole molecule 009-030-012
Product Information
Human Serum IgA, whole molecule 009-030-011
Product Information
Mouse IgG, whole molecule 015-030-003
Product Information
Mouse IgG, F(ab')2 fragment 015-030-006
Product Information
Mouse IgG, Fab fragment 015-030-007
Product Information
Mouse IgG, Fc fragment 015-030-008
Product Information
Rabbit IgG, whole molecule 011-030-003
Product Information
Rat IgG, whole molecule 012-030-003
Product Information
Sheep IgG, whole molecule 013-030-003
Product Information
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