LBIS™ Rat Urinary Albumin Assay Kit
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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60Tests
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250Tests
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In stock in Japan |
Document
Product Overview
Albumin is a plasma protein synthesized mainly in the liver, and measuring albumin concentration in serum and plasma is an important indicator in the diagnosis and management of liver disease and liver dysfunction. It is also known that in kidney diseases characterized by protein leakage into the urine, total protein and albumin concentrations in the blood decrease, causing hypoalbuminemia. Urinary albumin measurements are commonly performed using TIA method. Fujifilm Wako offers TIA reagents optimized for automated analyzers compatible with specimens from rats and mice.
This kit is a research reagent for an autoanalyzer to measure albumin in rat urine or serum using anti-rat albumin antibody. This is intended for research use only.
Kit Performance
| Analysis sample | Rat Urine/Serum* |
|---|---|
| Calibration curve range | 6.17-500 μg/mL |
| Sample volume | 6 μL |
| Measurement duration | Approx. 10 min. |
Kit Component
| Components | 60 tests | 250 tests |
|---|---|---|
| 1) Reagent 1 | 18.5 mL / 1 bottle | 57.8 mL / 1 bottle |
| 2) Reagent 2 | 6.6 mL / 1 bottle | 16.5 mL / 1 bottle |
| 3) Rat Urinary Albumin Standard | 2 mL / 1 bottle | 1.5 mL / 2 bottles |
| 4) Buffer for Standard | 4 mL / 1 bottle | 5 mL / 2 bottles |
Data
Repeatability (within run precision)
Repeatability was conducted by measuring five-fold assays of serum samples from rat at three concentrations with this product.
| n＼ID | Rat (S.D. rat (8 weeks old ♂)) serum | ||
|---|---|---|---|
| A (μg/mL) | B (μg/mL) | C (μg/mL) | |
| 1 | 387 | 215 | 25.9 |
| 2 | 385 | 218 | 26.7 |
| 3 | 389 | 213 | 25.2 |
| 4 | 382 | 209 | 24.4 |
| 5 | 388 | 216 | 25.1 |
| mean | 386 | 214 | 25.5 |
| SD | 2.77 | 3.42 | 0.873 |
| CV(%) | 0.7 | 1.6 | 3.4 |
[Result]
The CV (%) of rat serum was 0.7-3.4%, indicating good repeatability.
Reproducibility (between run precision)
Reproducibility was conducted by measuring serum samples from rat at three concentrations each for four days with this product.
| Day＼ID | Rat (S.D. rat (8 weeks old ♂)) serum | ||
|---|---|---|---|
| D (μg/mL) | E (μg/mL) | F (μg/mL) | |
| 1 | 256 | 115 | 18.7 |
| 2 | 251 | 121 | 18.1 |
| 3 | 252 | 119 | 19.2 |
| 4 | 250 | 118 | 19.3 |
| mean | 252 | 118 | 18.8 |
| SD | 2.63 | 2.50 | 0.550 |
| CV(%) | 1.0 | 2.1 | 2.9 |
[Result]
The CV (%) of mouse serum was 1.0-2.9%, indicating good reproducibility.
Dilution Linearity Test
Serum from rat were serially diluted twofold with the buffer in this kit and measured in triplicate to assess linearity.
[Result]
All measurement results showed good linearity within the measurement range.
Spike and Recovery Test
Standard solutions at four concentrations were added to serum from rat. Next, spike recovery tests were conducted, with each measurement performed in duplicate.
Rat (S.D. rat (8 weeks old ♂)) sample
| Spiked amount (μg/mL) |
Measurement value (μg/mL) |
Recovery volume (μg/mL) |
Recovery rate (%) |
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|---|---|---|---|---|
| Serum-1 | 0 | 12.8 | - | - |
| 15 | 27.1 | 14.3 | 95.3 | |
| 30 | 41.4 | 28.6 | 95.3 | |
| 60 | 70.7 | 57.9 | 96.5 | |
| 120 | 130 | 117 | 97.7 | |
| Average | 96.2 | |||
| Spiked amount (μg/mL) |
Measurement value (μg/mL) |
Recovery volume (μg/mL) |
Recovery rate (%) |
|
|---|---|---|---|---|
| Serum-2 | 0 | 58.1 | - | - |
| 100 | 155 | 96.9 | 96.9 | |
| 150 | 207 | 149 | 99.3 | |
| 200 | 260 | 202 | 101 | |
| 250 | 318 | 260 | 104 | |
| Average | 100 | |||
[Result]
Good recovery rates were confirmed.
FAQ
About sample
- What should I do with samples that exceed the measurable range?
- Dilute samples with purified water for an autoanalyzer, or dilution of samples should be made in a test tube using saline solution. Obtained value should be raised its dilution rate to be assay value.
- Is it possible to conduct measurements using chylous samples or hemolyzed samples?
- Hemolytic and chylous serum samples are not suitable.
* To avoid influence of blood (high lipid or hemolysis, etc.), if your original samples have heavy chyle or hemolysis, do not use them for assay. Abnormal value might be obtained with hemolysis above 200 mg/dL with this kit.
About kit usage
- What instruments, and equipment are required for the assay using this kit?
- An autoanalyzer
- Purified water (distilled water)
- Physiological saline
- Test tubes for preparation of standard solution series
- Pipettes (disposable tip type)
- A vortex-type mixer.
- What are the storage conditions guidelines for each reagent?
- Reagent 1
Store at 2°C - 10°C. - Reagent 2
Store at 2°C - 10°C. - Mouse Urinary Albumin Standard
Store the unused reagents at 2°C - 10°C. - Buffer for Standard
Store at 2°C - 10°C.
- Reagent 1
Overview / Applications
Property
Manufacturer Information
Alias
- 634-25301(previous code)
630-25303(previous code)
AKRAL-020S(previous code)
AKRAL-020SZ1(previous code)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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