iMatrix-411

• Highly pure recombinant protein product of human laminin 411-E8 fragment expressed by CHO-S cells
• iPS cells cultured with iMatrix-511 can be subsequently cultured in iMatrix-411 to promote their differentiation into vascular endothelial cells
• Cells can be cultured in a feeder-free condition

hiPS cells successfully differentiated into vascular endothelial cells with high efficiency by using laminin 411-E8 fragment; iMatrix-411

A research group led by Dr. Megumu Saito (Center for iPS Cell Research and Application (CiRA), Kyoto University) and Dr. Kiyotoshi Sekiguchi (Endowed Research Section, Institute for Protein Research, Osaka University) developed a technique to efficiently induce differentiation of iPS cells into fully functional vascular endothelial cells using laminin 411-E8 fragment as a scaffold. The study was published in Scientific Reports.

Reference

Ohta R. et al. “Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells” Scientific Reports 6, Article number: 35680 (2016).

1. Dilute iMatrix-411 with PBS(-) to the final concentration for coating the culture surface.
   ※The recommended concentration for coating is 0.4 µg/cm².
   ※The optimal concentration for coating varies with the type of cells, cell lines, and the culture medium used. For the first time usage, start with the final concentration of 0.4 µg/cm2 and determine the optimal condition for coating.
2. Dilute iMAtrix411 quickly. If using tubes etc. for dilution, we recommend low protein-binding tubes.
   ※Leave for 1 hour at 37 °C, 3 hours in room temperature, or overnight at 4°C to allow coating of the culture surface by iMatrix411.
3. Remove the iMAtrix-411 solution after coating, and immediately seed the cells to avoid drying of the surface.
   ※Try not to let the coating surface dry out.

For a 6-well plate (9.6 cm2/ well), add 7.68 μL of iMatrix-411 and 1.99 mL of PBS (-) per well (1.92 μg/mL, 2 mL/well). Use the plate immediately after coating.