Exosome ELISA Kits
Fujifilm Wako offers a line-up of ELISA kits that can be used for qualitative and quantitative analysis of extracellular vesicles (EV), including exosomes. Fujifilm Wako provides high-sensitivity ELISA kits that utilize "PS-affinity method" and ELISA kits that use anti human CD9, CD63, or CD81 antibody. From these, you can select a product that meets your purpose.
Selection Table for Exosome ELISA Kit
| ries | PS CaptureTM Exosome ELISA Kit | CD9/63/81-Capture Human Exosome ELISA Kit | |||
|---|---|---|---|---|---|
| Product Number | 297-79201 | 298-80601 | 296-83701 | 290-83601 | 292-83801 |
| Product Name | PS CaptureTM Exosome ELISA Kit (Anti Mouse IgG POD) | PS CaptureTM Exosome ELISA Kit (Streptavidin HRP) | CD9-Capture Human Exosome ELISA Kit (Streptavidin HRP) | CD63-Capture Human Exosome ELISA Kit (Streptavidin HRP) | CD81-Capture Human Exosome ELISA Kit (Streptavidin HRP) |
| Package Size | 96 tests | 96 tests | 96 tests | 96 tests | 96 tests |
| Capture molecule | Tim4 protein | Tim4 protein | Anti human CD9 antibody (rat) |
Anti human CD63 antibody (mouse) |
Anti human CD81 antibody (mouse) |
| Detection Antibody(included in the kit) | Anti human CD63 antibody (mouse) |
Anti human CD63 antibody (mouse) |
Anti human CD9 antibody (rat) |
Anti human CD63 antibody (mouse) |
Anti human CD81 antibody (rat) |
| Enzyme conjugation method | Secondary antibody | Biotin -Streptavidin |
Biotin -Streptavidin |
Biotin -Streptavidin |
Biotin -Streptavidin |
| Sample | Purified exosome Culture supernatant*1 | Purified exosome Culture supernatant Body Fluid (Serum, Plasma etc.) |
Purified exosome Culture supernatant Body Fluid (Serum, Plasma etc.) |
Purified exosome Culture supernatant Body Fluid (Serum, Plasma etc.) |
Purified exosome Culture supernatant Body Fluid (Serum, Plasma etc.) |
| Species | Human, Mouse, Rat etc.*2 | Human, Mouse, Rat etc.*2 | Human | Human | Human |
| Analyte | PS positive exosome*3 | PS positive exosome*3 | Human CD9 positive exosome | Human CD63 positive exosome | Human CD81 positive exosome |
*1 Since the secondary antibody for detection reacts nonspecifically with human, mouse, and rat IgG, serum or plasma samples cannot be used for measurement.
*2 The antibody included in the kit is an anti human CD63 antibody. Use appropriate antibodies when measuring non-human samples.
*3 If exosome-depleted FBS is not used, PS-positive exosomes derived from FBS are also detected.
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More Information
Application of the PS affinity method to ELISA
Fujifilm Wako developed PS Capture™ Exosome ELISA Kit by applying affinity binding of Tim4 protein with exosomes. This kit can detect exosomes at a sensitivity higher than that of conventional ELISA methods immobilization of antibodies against exosome surface markers. Exosomes in samples such as culture supernatant and serum are captured by Tim4 protein on a dry plate in the presence of calcium ion. The captured exosomes are detected by a primary antibody against an exosome surface marker protein and a labeled secondary antibody. While a mouse anti-CD63 monoclonal antibody is provided with the kit, a user-provided mouse primary antibody against any other exosome surface marker may also be used for exosome detection.
The greatest feature of this kit is that it provides exosomes detection with higher sensitivity than that of western blotting analysis and conventional product for exosome ELISA. First, the detection limit for exosomes in western blotting was examined for comparison with this kit (Figures 1a and b). Western blot analysis of exosomes purified from COLO201 cells (of human colon adenocarcinoma origin) with an anti CD63 monoclonal antibody detected exosomes in an amount as small as 75 ng on the protein basis. Next, the detection limits of this kit for exosomes purified from K562 cells (of human leukemia origin) and COLO201 cells were determined to be 49.9 pg and 10.9 pg, respectively, demonstrating that this kit had a detection sensitivity more than 1,000 times higher than that of western blotting. Considering that the detection limits of conventional products for exosome ELISA range approximately several ng to several μg (refer to instruction manuals for individual products), the present results demonstrated that this kit utilizing affinity binding of exosomes to Tim4 via PS has a sensitivity more than 100 times higher than those of conventional ELISA methods involving immobilization of an antibody against an exosome surface protein marker.
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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