Peptidoglycan and β-D-Glucan-Specific SLP Reagents
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Test Principle
The SLP reagent strongly reacts with PG and BDG, inducing formation of black melanin pigment, which can be monitored, either through visual detection, or quantitatively by measuring the absorbance at 650 nm.
For latter application the analysis can be performed by using a microplate reader or in combination with the Toxinometer® ET-7000.
The quantification is achieved through monitoring of melanin formation by measuring the activation time (Ta or onset time) of the reaction, i.e. the point in time at which the absorbance reaches a predetermined threshold. By correlating the measured value to a previously generated calibration curve with a PG standard, the absolute concentration of PG and BDG can be determined with a sensitivity in the lower pg/ml-1 range.
Optical system for quantitative analysis of pyrogens (PG and/or BDG)
through kinetic colorimetric assay and reaction time course.
Testing Procedure
SLP-HS single reagent in combination with Toxinometer® ET-7000
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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