For rheumatology and tissue fibrosis research

Anti CTGF Antibodies

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CTGF is a secreted protein of about 38 kDa produced from umbilical veins and vascular endothelial cells . CTGF is consist of four modules, and regulates chondrocyte proliferation / differentiation and intercellular adhesion. Some reports showed that CTGF is involved in various diseases such as cancer, renal failure, liver failure and rheumatism.

This items are mouse monoclonal antibodies established by DNA immunization, specifically react with native form CTGF. it has neutralizing activity.

Features

  • CTGF Neutralizing Activity
    Suppression of arthritis model animals (mice)
  • Each 4 clone recognizes
    Each module 1, 2, 3 and 4, respectively

Epitope Clone No. Cross- reactivity Subclass Applications Concentration(1st lot)
Module1 30D2 Human, Mouse Mouse IgG1 in vitro neutralization
in vivo neutralization
ELISA
0.8 mg/mL
Module2 2-3 Human, Mouse Mouse IgG1 in vitro neutralization 0.9 mg/mL
Module3 3-54 Human, Mouse Mouse IgG1 in vitro neutralization 1 mg/mL
Module4 4-69 Human, Mouse Mouse IgG1 in vitro neutralization 0.7 mg/mL

in vitro neutralization assay

Inhibition of osteoclastognenesis

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Conditions
  1. PBMCs are collected from whole human blood and CD14+ cell is isolated.
  2. CD14+ cells are cultured for 7days (37℃, 5%CO2) with α-MEM (add 10% FBS) ,M-CSF (25ng/mL), sRANKL (40ng/mL)and antiCTGF Abs.
  3. Osteoclasts (TRAP positive cells) were counted by optical microscopy.

Result

Enhancement of osteoclastogenesis by CTGF were repressed all CTGF antibodies.

This data was provided by Dr. Miyashita and Dr. Sekigawa of Institute for Environmental and Gender Specific Medicine, Juntendo University in Japan

in vivo neutralization assay

Suppression of arthritis model animals (mice)

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Arthritis point criteria
POINT Observation
0 No change
1 Regional reddening of the limbs, or swelling and reddening of one finger.
2 Swelling of limbs' joints, or swelling of two or more fingers.
3 Medium - prominents swelling of hands and feet.
4 Remarkable swelling and redding of limbs, or hardening.

※The above point is the total point of the limbs.

Conditions

DBA/1J mice (Female, 7-8 weeks of age) were immunized intradermally at the base of the tail with 100 μL of emulsion (in which emulsified in equal amounts of bovine type II collagen and Freund’s complete adjuvant (CFA) ).
The anti-CTGF antibody was intraperitoneally administered 1 week before the start of immunization, and administered once a week for a total of 8 times.
Evaluation of limb arthritis was performed once a week until immunization for 8 weeks.
(Refer to the table below for evaluation criteria)

Result

Mice treated with anti-CTGF module1 Ab significantly suppressed arthritis compared to the untreated mice with collagen induced arthritis.

This data was provided by Dr. Nozawa and Dr. Sekigawa of Institute for Environmental and Gender Specific Medicine, Juntendo University in Japan

References

  1. Ikawa ,Y., et al.: J. Cell Physiol., 216, 3, 680(2008).
  2. Nozawa, K., et al.: Arthritis Rheum., 65, 6, 1477 (2013).
  3. Miyashita, T., et al.: Autoimmunity., 49, 2,109 (2016).
  4. Miyazaki O, et al.: Ann. Clin. Biochem., 47, 205(2010).

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

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