Limulus Color KY Series (LAL Chromogenic Reagent)
Limulus Color KY series is intended for the detection of endotoxin specific by Quantitative Kinetic Chromogenic Assay(KCA).
These series offer a highly sensitive measurement.
- Endotoxin-specific lysate, avoids false positive results from glucans
- Available in multi-tests vials or single-test vials
- Quantitative Kinetic-Chromogenic Assay (KCA) reagent
- KCA can be performed in tube reader or microplate reader
- KCA quantitative range detection limit of 0.0002 EU/mL (Single test) and 0.0005 EU/mL (Multi test).
- Available with matched control standard endotoxin (CSE)
- 0.1 mL or 0.2 mL sample size when used with tube reader:0.05 mL sample size when used with microplate reader
| Single test: | The lysate reagent for one measurement is dispensed in advance in a test tube. |
|---|---|
| Multi test: | The lysate reagent which dissolve in water for BET(bacterial endotoxin test), dispense the necessary amount in reaction tubes or plates. |
Principle
The reagent is based on the color development mechanism, which is activated by the presence of endotoxin. First, a series of activations of serine protease precursors in the reagent occur, consequently triggering the final reaction, in which a clotting enzyme hydrolyzes the chromogenic substrate to release a yellow chromogen (pNA).
Color Development Cascade Mechanism of the Limulus Color Reagent
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Tube Reader
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Microplate Reader
Wako Blog: Talking of LAL
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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