Exosomes, from COLO201 cells, purified
Features
- High purity
- Long-term stability
- Ready-to-use liquid format
- CD9, CD63 and CD81 positive
Specifications
- Physical State:Liquid
- Constituent:Exosomes, TBS, EDTA, Polymer as a stabilizer
- Concentration:10 μg/mL*
*The value is corrected protein concentration by a CD63 signal.
Detecting exosome surface markers by Western blot
10 ng of “Exosomes, from COLO201 cells, purified” was loaded on each well and SDS-PAGE was performed under non-reducing conditions. Then, canonical exosome surface markers such as CD9, CD63 and CD81 were detected using anti-CD9, anti-CD63 and anti-CD81 antibodies.
![01689_img01.png](images/01689_img01.png)
Primary antibodies
- Anti CD9, Monoclonal Antibody (1K) (Product code: 014-27763)
- Anti CD63, Monoclonal Antibody (3-13) (Product code: 012-27063)
- Anti CD81, Monoclonal Antibody (17B1) (Product code: 011-27773)
Secondary antibody
- HRP conjugated anti-Mouse IgG
CD9, CD63 and CD81 are highly expressed on “Exosomes, from COLO201 cells, purified” .
Next, 10 ng of “Exosomes, from COLO201 cells, purified” and five on-market Exosomes were loaded on each well and SDS-PAGE was performed under non-reducing conditions. Then, CD63 was detected using anti-CD63 antibodies.
![01689_img02.png](images/01689_img02.png)
Lane No.
① “Exosomes, from COLO201 cells, purified”
② Competitor 1: Exosomes, from A549 cells
③ Competitor 2: Exosomes, from HEK293 cells
④ Competitor 3: Exosomes, from A549 cells
⑤ Competitor 4: Exosomes, from COLO1 cells
⑥ Competitor 5: Exosomes, from HCT116 cells
Primary antibody
Anti CD63, Monoclonal Antibody (3-13) (Product code: 012-27063)
Secondary antibody
HRP conjugated anti-Mouse IgG
Our “Exosomes, from COLO201 cells, purified” only showed a CD63 signal compared to five on-market Exosomes in detecting the presence of CD63.
Particle size analysis using NanoSight
Comparison of particle size analysis between “Exosomes, from COLO201 cells, purified” and Exosomes from Competitor 1 using NanoSight.
“Exosomes, from COLO201 cells, purified” have expected particle size distribution.
Effect of repeated freeze-thaw on exosome structure
Structure of “Exosomes, from COLO201 cells, purified” which have been frozen at -20℃ and thawed at room temperature 15 times was observed by an electron microscope.
![01689_img05.png](images/01689_img05.png)
“Exosomes, from COLO201 cells, purified” was maintained their structure even they have undergone 15 times freeze-thaw cycles.
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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