Column for ADRA

Wakopak® Core C18 ADRA

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This product is a column suitable for the analysis of ADRA(OECD TG442C). It contains core-shell filler of 2.6μm in particle diameter.
It is checked for each column that NAC, NAL and other chemicals are respectively separated.

Column Performance Report

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Each column has a “Column Performance Report”.

Analysis Examples

Analysis examples of 10 proficiency substances recommended in OECD Test guideline.

Green:Chemical  Red:NAC or NAL  Blue:Chemical + NAC or NAL

  1. p-Benzoquinone
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  2. Sodium p-Toluenesulfonchloramide Trihydrate
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  3. Cinnamaldehyde
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  4. Palmitoyl Chloride
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  5. Imidazolidinyl Urea
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  6. Famesal
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  7. Glycerin
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  8. Benzyl Alcohol
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  9. Dimethyl Isophthalate
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  10. Propyl p-Hydroxybenzoate
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Green:Chemical  Red:NAC or NAL  Blue:Chemical + NAC or NAL

HPLC Conditions

Instrument Agilent UHPLC 1290 infinity Ⅱ (Ailent)
Column Wakopak® Core C18 ADRA 3.0 x 150 mm (D)
Mobile phase A 0.1 % Trifluoroacetic Acid-Water
Mobile phase B 0.1 % Trifluoroacetic Acid-Acetonitrile
NAC Gradient conditions 0min.(A:B=70:30)→9.5min.(45:55)→10.0min(0:100) →13.0min.(0:100)→13.5min.(70:30)→25.0min.(70:30)
NAL Gradient conditions 0min.(A:B=80:20)→9.5min.(55:45)→10.0min(0:100)→13.0min.(0:100)→13.5min.(80:20)→25.0min.(80:20)
Flow rate 0.3 mL/min
Mixer volume 35 μL
Column temperature 40 ℃
UV detector UV 281 nm
Injection Quantity 10 μL

 

The details of ADRA Kit

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ADRA Kit

Necessary reagents in addition to kit and column

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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