Column for ADRA

Wakopak® Core C18 ADRA

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This product is a column suitable for the analysis of ADRA(OECD TG442C). It contains core-shell filler of 2.6μm in particle diameter.
It is checked for each column that NAC, NAL and other chemicals are respectively separated.

Column Performance Report

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Each column has a “Column Performance Report”.

Analysis Examples

Analysis examples of 10 proficiency substances recommended in OECD Test guideline.

Green:Chemical  Red:NAC or NAL  Blue:Chemical + NAC or NAL

  1. p-Benzoquinone
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  2. Sodium p-Toluenesulfonchloramide Trihydrate
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  3. Cinnamaldehyde
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  4. Palmitoyl Chloride
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  5. Imidazolidinyl Urea
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  6. Famesal
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  7. Glycerin
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  8. Benzyl Alcohol
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  9. Dimethyl Isophthalate
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  10. Propyl p-Hydroxybenzoate
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Green:Chemical  Red:NAC or NAL  Blue:Chemical + NAC or NAL

HPLC Conditions

Instrument Agilent UHPLC 1290 infinity Ⅱ (Ailent)
Column Wakopak® Core C18 ADRA 3.0 x 150 mm (D)
Mobile phase A 0.1 % Trifluoroacetic Acid-Water
Mobile phase B 0.1 % Trifluoroacetic Acid-Acetonitrile
NAC Gradient conditions 0min.(A:B=70:30)→9.5min.(45:55)→10.0min(0:100) →13.0min.(0:100)→13.5min.(70:30)→25.0min.(70:30)
NAL Gradient conditions 0min.(A:B=80:20)→9.5min.(55:45)→10.0min(0:100)→13.0min.(0:100)→13.5min.(80:20)→25.0min.(80:20)
Flow rate 0.3 mL/min
Mixer volume 35 μL
Column temperature 40 ℃
UV detector UV 281 nm
Injection Quantity 10 μL

 

The details of ADRA Kit

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ADRA Kit

Necessary reagents in addition to kit and column

Product content may differ from the actual image due to minor specification changes etc.

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