StemSure® on-feeder hPSC Medium
StemSure® on-feeder hPSC medium is a liquid medium used for culturing human ES/ iPS cells on feeder cells. Little preparation is needed as it is a pre-mixed medium that contains the basal medium, serum replacements, non-essential amino acids, and reducing agents. bFGF should be added prior to use as it is not contained in the mixture.
- Pre-mixed medium, ready-to-use with the addition of bFGF
- Osmotic pressure
- Sterility test
- Endotoxin test
- Mycoplasma test
- Cell proliferation assay
- Alkaline phosphatase staining
Colony Formation and ALP Staining 1
Human iPS cell line 201B7 maintained in a feeder-free medium was transferred to be cultured on feeder cell layer in either StemSureStemSure on-feeder hPSC medium or an equivalent product from another company. Morphology of the colonies and alkaline phosphatase (ALP) staining pattern were examined after 5 passages (see below). ALP staining demonstrated that there were no colonies with differentiated cells even after 5 passages.
There were no abnormal findings in terms of the morphology of colonies for cells grow in StemSureStemSure on-feeder hPSC medium after 15 passages (final concentration of bFGF: 5 ng/mL, coating: gelatin).
Colony Formation and ALP Staining 2
Human iPS cell line 201B7 was cultured for 19 passages in either StemSureStemSure on-feeder hPSC medium or an equivalent product from another company. Using our product, undifferentiated state of the cells was maintained for a prolonged period of time.
(※ % indicated in the right bottom corner of ALP images indicate the % of differentiated colonies.)
Human iPS cell line 201B7 was cultured in StemSureStemSure on-feeder hPSC medium. Cell proliferation was observed for 3 days using IncuCyte ZOOM.
Identifying expression of markers for undifferentiated cells
Human iPS cell line 201B7 maintained in a feeder-free medium was transferred to be cultured on feeder cell layer in StemSureStemSure on-feeder hPSC medium. After 5-6 passages, cells were stained with markers of undifferentiated cells (OCT3/4, SOX2, TRA-1-81, and BC2LCN) to confirm that undifferentiated states were maintained.
※BC2LCN is a recombinant lectin that is highly specific to sugar chains on the surface of undifferentiated human ES/ iPS cells.
Confirming differentiation into three germ lineages
Human iPS cell line 201B7 was cultured in StemSureStemSure on-feeder hPSC medium for 9 passages, and were seeded on a gelatin-coated dish 7 days after the formation of EB. Cells were cultured for 14 days, and the expressions of βⅢ-Tublin, α-SMA, and AFP were examined to confirm differentiation into three germ lineages.
- Open All
- Close All
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.