MF-registered Small Molecules

ROCK inhibitor

Y-27632 improves the viability and cloning efficiency of human ES/ iPS cells after cryopreservation. Furthermore, Y-27632 can be used with EGF, CHIR99021, A-83-01, and SB431542 to establish Trophoblast stem (TS) cells from human blastocysts.

＜Product Description＞

• Appearance: white to pale yellow, crystalline powder to powder
• Assay (HPLC): min. 98.0%
• Solubility: soluble in water
• Specific rotation [α]D20(c=1.0,CH3OH) ：+2.0°～+10.0°
• Endotoxin^※: less than 0.25 EU/mg
• Tested negative for mycoplasma^※
• Tested for viable bacteria^※
• CAS RN^®: 331752-47-7
• C₁₄H₂₁N₃O・2HCl・H₂O=338.27
• [Registration number: 227MF40013]

References

1. Uehata, M., et al. : Nature, 389, 990 (1997).
2. Sakamoto, K., et al. : J. Pharmacol. Sci., 92, 56 (2003).
3. Nishimaru, K., et al. : J. Pharmacol. Sci., 92, 424 (2003).
4. Watanabe, K., et al. : Nat. Biotechnol., 25, 681 (2007).
5. Martin-Ibanez, R., et al. : Hum. Reprod., 23, 2744 (2008).
6. Claassen, DA., et al. : Mol. Reprod. Dev., 76, 722 (2009).
7. Kawamata, M., et al. : Proc. Natl. Acad. Sci. USA., 107, 14223 (2010).
8. Ito, H., et al. : Liver Int., 32, 592 (2012).
9. Katsuda,T., et al. : Cell Stem Cell, 20, 41 (2017).
10. Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
11. Ogawa, K., et al. : Sci. Rep., 8, 3615 (2018).
12. Ayabe, H., et al. : Stem Cell Reports., 11, 306 (2018).

Inhibitor of ALK4, ALK5, and ALK7

A-83-01 enables long-term and consistent culture of rat and canine iPS cells in a state maintaining the undifferentiated state.. Furthermore, the use of A-83-01 with Y-27632 and CHIR99021 induces reprogramming of rat and mouse mature hepatocytes into liver progenitors that can be cultured stably in vitro

＜Product Description＞

• Appearance: white to yellow, crystalline powder to powder or mass
• Assay (HPLC): min. 98.0%
• Solubility: soluble in DMSO
• Endotoxin^※: less than 0.01 EU/mg
• Tested negative for mycoplasma^※
• Tested for viable bacteria^※
• CAS RN^®: 909910-43-6
• C₂₅H₁₉N₅S=421.52
• [Resistration number: 229MF40005]

References

1. Li, W., et al. : Cell Stem Cell, 4, 16 (2009).
2. Katsuda ,T., et al. : Cell Stem Cell, 20, 41 (2017).
3. Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).

GSK-3β Inhibitor

It has been reported that differentiation can be suppressed with high efficiency when ES cells are cultured in a medium containing CHIR99021 and PD0325901.

Product Description

• Appearance: white～pale brown, crystalline powder～powder
• Assay (HPLC): min. 98.0%
• Solubility: soluble in methanol
• Water: max. 1.0%
• Endotoxin^※: less than 0.05EU/mg
• Tested negative for mycoplasma^※
• Tested for viable bacteria^※
• CAS RN^®: 252917-06-9
• C₂₂H₁₈Cl₂N₈ = 465.34
• Registration number: 231MF40003

References

1. Ying, QL., et al.: Nature, 453, 519 (2008).
2. Fujimori, K., et al.: Stem Cell Reports., 9, 1675 (2017).

Inhibitor of ALK4, ALK5, and ALK7

SB431542 promotes proliferation, differentiation and sheet formation of ES cells-derived endothelial cells. It also improves reprogramming efficiency and speed when used with PD0325901 and thiazovivin. It has also been reported that cells induced by SB43154, CHIR99021, and dorsomorphin have significantly improved differentiation efficiency and speed.

＜Product Description＞

• Appearance: white to yellowish brown, crystalline powder to powder
• Assay(HPLC): min. 98.0%
• Solubility: soluble in DMSO
• Endotoxin^※: less than 0.05 EU/mg
• Tested negative for mycoplasma^※
• Tested for viable bacteria^※
• CAS RN^®: 301836-41-9
• C₂₂H₁₆N₄O₃=384.39
• [Resistration number: 228MF40016]

References

1. Ogawa, K., et al. : J. Cell. Sci.,120, 55 (2007).
2. Lin T., et al. : Nat. Methods, 6, 805 (2009).
3. Fujimori, K., et al. : Stem Cell Reports., 9, 1 (2017).
4. Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).

• Tests for mycoplasma, endotoxin, and viable bacteria are performed for every lot as part of standard tests; however, the results are not required for MF registration.
• Registration in the Drug Master File (MF) does not indicate that the product was tested for its quality and safety by the Ministry of Health, Labor and Welfare (Pharmaceuticals and Medical Devices Agency).