Ninhydrin Coloring Solution Kit-Ⅱ for JEOL
- for JEOL-Amino Acid Automated Analyzer
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep below 25 degrees C.
- GHS :
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Ninhydrin Color Development Method
Ninhydrin reaction is a method to detect and quantify amino acids via ninhydrin coloration.
Amino acids reacted with ninhydrin reaction reagent are detected using two wavelengths, i.e., 570 nm and 440 nm.
The primary amine amino acid (α-amino acid) is colored purple at 570 nm, while the secondary amine (imino acid) amino acid is colored yellow at 440 nm.
Protocol
Methods for amino acid analysis include precolumn derivatization and postcolumn derivatization employed by ninhydrin. Postcolumn derivatization is a method in which a sample is reacted with the derivatization reagent after column separation and transferred to the detector.
Flow diagram of ninhydrin method1)
Measurement Principle
- The sample is pressured into an alkaline-cation exchange resin. When the eluent pH is increased in a stepwise manner, a higher amount of acidic amino acid is eluted early.
- When this eluate is heated after the addition of Ninhydrin Coloring Solution, a colored substance showing the local maximum absorption is produced at 570 nm for α-amino acid and 440 nm for imino acid.
- The peak area of this colored substance is compared with that of the standard solution to determine the concentration of each amino acid in the sample.
- Ito, M. et al. : Wako Jun-yaku Jiho, 86 (3), 15 (2018).
Amino Acid Automated Analyzer Manufactured by JEOL: JLC-500/V
Features
- It provides highly accurate and reproducible data
Highly accurate and reproducible data can be obtained due to technologies, such as vacuum degasser to remove dissolved gas in buffer solution, pump with constant-flow sensor feedback, phase-control pulse mixing type that can efficiently mix ninhydrin and eluent. - It employs a tandem column
It employs a tandem column (multi-stage laminated pressure dispersion column) that prevents the resin from crushing and clogging owing to high pressure and high speed, and this extends the column service life. - It is compatible with GLP
Software installed includes those for equipment performance validation (instrument, analytical method); system suitability test during routine analysis; equipment maintenance; analytical method change; and performance check.
Manufacturer's Website: JEOL Website
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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