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Anti Oxytocin, Guinea Pig

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
  • Structural Formula
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SDS
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Distributor
017-28571
Barcode No
4548995098566
50uL
List Price
JPY 44,000

In stock in Japan

Document

SDS
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Product Overview

Oxytocin is a 9-amino acid peptide hormone produced in the paraventricular hypothalamic nucleus and released from the posterior pituitary gland1). Oxytocin promotes uterine contraction and breast milk secretion, as well as being involved in stress relief and the activation of maternal behavior, so it is often referred to as the happiness hormone or the love hormone2). "Anti Oxytocin, Guinea Pig" is a guinea pig polyclonal antibody that reacts with Oxytocin3).

Antibody Information

Clonality Polyclonal
Antigen KLH conjugated synthetic peptide (CYIQNCPLG-NH2)
Host Guinea pig
Formulation Antiserum diluted in PBS
Conjugate Unconjugated
Cross-reactivity Human, Mouse, Rat, Bovine
Application Immunohistochemistry (Frozen Section) 1:1,500

Protocol (Example)

1. Perfusion fixation Perfuse with sodium citrate/PBS to remove blood, followed by perfusion with 4% PFA.
2. Post-fixation 4% PFA (24 hours)
3. Immersion in sucrose 30% Sucrose/PBS (4℃, overnight ~ approximately 2 days)
4. Preparation of frozen sections After removing sucrose, prepare frozen blocks and cut 30-μm thick sections using a cryostat.
5. Glycine treatment 25 mM glycine/PBS (approximately 20 minutes)
6. Blocking 5% normal goat serum in 0.3% Triton X-100/PBS (4℃, overnight)
7. Primary antibody reaction Anti Oxytocin, Guinea pig (1:1,500), 1% normal goat serum in 0.3% Triton X-100/PBS (4℃, overnight ~ 2 days).
8. Washing 0.3% TritonX-100/PBS (5 minutes x 3)
9. Secondary antibody reaction Alexa Fluor® 488 AffiniPure Goat Anti-Guinea Pig IgG (H+L) (1:500) (Jackson ImmunoResearch, Product Number: 106-545-003), 1% normal goat serum in 0.3% Triton X-100/PBS (room temperature, 2 hours)
10. Washing 0.3% TritonX-100/PBS (5 minutes x 3)
11. Mounting Store at 4℃ in a dark place.

Data

Application Data

Immunohistochemistry

Immunohistochemistry

Species: Mouse
Site: Paraventricular hypothalamic nucleus
Sample: Frozen section
Antibody concentration: 1:1,500

Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology

References

FAQ

About protocol

What secondary antibodies can be used?
At Fujifilm Wako, the following secondary antibodies have been successfully used:
Alexa Fluor® 488 AffiniPure Goat Anti-Guinea Pig IgG (H+L) (Jackson Immuno Research, Product Number: 106-545-003)

About application

What animals can this antibody be used for?
Fujifilm Wako has confirmed that it can be used for humans, mice, rats and bovines. Other species have not been tested.

Overview / Applications

Outline Oxytocin is a 9-amino acid peptide hormone produced in the paraventricular hypothalamic nucleus and released from the posterior pituitary gland. Oxytocin promotes uterine contraction and breast milk secretion, as well as being involved in stress relief and the activation of maternal behavior, so it is often referred to as the happiness hormone or the love hormone. Anti Oxytocin, Guinea Pig is a guinea pig polyclonal antibody that reacts with Oxytocin.

[Antigen] KLH conjugated synthetic peptide corresponding to the oxytocin
[Species cross reactivity] Human, mouse, rat and bovine
[Application] Immunohistochemistry (frozen section) 1:1500
Precautions for Use Avoid repeated freeze and thaw.

Property

Appearance Liquid
Composition Antiserum diluted in PBS

Manufacturer Information

Alias

  • Anti Oxytocin, Guinea Pig Polyclonal Antibody

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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