Anti Parvalbumin, Guinea Pig
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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50uL
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In stock in Japan |
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Product Overview
Parvalbumin(PV) is a small calcium-binding protein with about 12 kDa involved in calcium signaling and plays a key role in second messenger systems and signal transduction in tissues such as skeletal muscle and brain1). PV is present in GABAergic interneurons and is expressed in multiple brain regions, including chandelier and basket cells in the cortex and Purkinje cells in the cerebellum2-4). As such, PV is widely used as a marker for general inhibitory neurons. “Anti Parvalbumin, Guinea Pig” is a guinea pig polyclonal antibody that reacts with PV5).
Antibody Information
| Clonality | Polyclonal |
|---|---|
| Antigen | Recombinant rat parvalbumin |
| Host | Guinea pig |
| Formulation | Antiserum |
| Conjugate | Unconjugated |
| Cross-reactivity | Mouse , Rat |
| Application | Immunohistochemistry (Frozen Section) 1:1,000-3,200 |
Protocol (Example)
| 1. Perfusion fixation | Perfuse with 0.1% sodium citrate/PBS to remove blood, followed by perfusion with 4% PFA. |
|---|---|
| 2. Post-fixation | 4% PFA (24 hours) |
| 3. Immersion in sucrose | 30% Sucrose/PBS (4℃, overnight ~ approximately 2 days) |
| 4. Preparation of frozen sections | After removing sucrose, prepare frozen blocks and cut 30-μm thick sections using a cryostat. |
| 5. Glycine treatment | 25 mM glycine/PBS (approximately 20 minutes) |
| 6. Blocking | 5% normal goat serum in 0.3% Triton X-100/PBS (4℃, overnight) |
| 7. Primary antibody reaction | Anti Parvalbumin, Guinea Pig (1:1,500), 1% normal goat serum in 0.3% Triton X-100/PBS (4℃, overnight ~ 2 days). |
| 8. Washing | 0.3% Triton X-100/PBS (5 minutes x 3) |
| 9. Secondary antibody reaction | Alexa Fluor® 488 AffiniPure Goat Anti-Guinea Pig IgG (H+L) (1:500) (Jackson Immuno Research, Product Number: 106-545-003), 1% normal goat serum in 0.3% Triton X-100/PBS (room temperature, 2 hours) |
| 10. Washing | 0.3% Triton X-100/PBS (5 minutes x 3) |
| 11. Mounting | Store at 4℃ in a dark place. |
Data
Application Data
Immunohistochemistry
Species: Mouse
Site: Sensory cortex
Sample: Frozen section
Antibody concentration: 1:1,500
Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology
Species: Mouse
Site: Cortex
Sample: Slice section
Antibody concentration: 1:1,000
Data by courtesy of
Dr. Hagiwara, Faculty of Science and Technology, Tokyo University of Science
Species: Mouse
Site: Piriform cortex
Sample: Slice section
Antibody concentration: 1:1,000
Data by courtesy of
Dr. Hagiwara, Faculty of Science and Technology, Tokyo University of Science
References
- Cates, M. S. et al.: Structure, 7(10), 1269(1999).
Metal-ion affinity and specificity in EF-hand proteins: coordination geometry and domain plasticity in parvalbumin - Taniguchi, H., Lu, J. and Huang, Z. J.: Science, 339(6115), 70(2013).
The spatial and temporal origin of chandelier cells in mouse neocortex - Schwaller, B., Meyer, M. and Schiffmann, S.: The cerebellum, 1, 241(2002).
‘New’ functions for ‘old’ proteins: The role of the calcium-binding proteins calbindin D-28k, calretinin and parvalbumin, in cerebellar physiology. Studies with knockout mice - Rudy, B., Fishell, G., Lee, S. and Hjerling‐Leffler, J.: Dev. Neurobiol., 71(1), 45(2011).
Three groups of interneurons account for nearly 100% of neocortical GABAergic neurons - Taniguchi, Y. et al.: Cell. Tissue Res., 343, 303(2011).
Localization of plasminogen in mouse hippocampus, cerebral cortex, and hypothalamus
FAQ
About protocol
- What secondary antibodies can be used?
- At Fujifilm Wako, the following secondary antibodies have been successfully used:
Alexa Fluor® 488 AffiniPure Goat Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch, Product Number: 106-545-003)
About application
- What animals can this antibody be used for?
- Fujifilm Wako has confirmed that it can be used for mice and rats. Other species have not been tested.
Overview / Applications
| Outline | Parvalbumin (PV) is a small calcium-binding protein with about 12kDa involved in calcium signaling and plays a key role in second messenger systems and signal transduction in tissues such as skeletal muscle and brain. PV is present in GABAergic interneurons and is expressed in multiple brain regions, including chandelier and basket cells in the cortex and Purkinje cells in the cerebellum. As such, PV is widely used as a marker for general inhibitory neurons. PV has also been reported to be involved in psychiatric disorders such as schizophrenia. Anti Parvalbumin, Guinea Pig is a guinea pig polyclonal antibody that reacts with PV. [Antigen] Recombinant rat parvalbumin [Species cross reactivity] Mouse and rat [Application] Immunohistochemistry (frozen section) 1:1,000-3,200 |
|---|---|
| Precautions for Use | Avoid repeated freeze and thaw. |
Property
| Appearance | Liquid |
|---|---|
| Composition | Antiserum |
Manufacturer Information
Alias
- Anti Parvalbumin, Guinea Pig Polyclonal Antibody
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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