For blood-brain barrier research

Anti Claudin-5, Monoclonal Antibody(R9/M48)

Claudin-5 is a tetra-transmembrane protein with approximately 23kDa, belongs to a member of Claudin family. It functions as a key component regulating tight junction of blood-brain barrier 1). These products are monoclonal antibodies which specifically recognize Claudin-5, and have neutralizing activity which reduces barrier function in blood-brain barrier model 1).They have no cross-reaction to Claudin-1-4,6,7 1).

1)Hashimoto, Y., et al.: J. Pharmacol. Exp. Ther., 363(2), 275(2017).

This product is sold under thelicense of Osaka University-Ehime University-FUJIFILM Wako Pure Chemical Corporation joint application patent.
PCT/JP2018/016975 [ANTI-CLDN-5 ANTIBODY, AND DRUG CONTAINING SAID ANTIBODY]

Antibody information

Anti Claudin-5, Monoclonal Antibody (R9) Anti Claudin-5, Monoclonal Antibody (M48)
Code No. 014-28101 011-28091
Clone No. R9 M48
Antigen Vector expressing human claudin-5 full length accession#AAH19290 Vector expressing human claudin-5 full length accession#AAH19290
Buffer PBS PBS
Subclass Rat IgG2b・κ Mouse IgG3・κ
Cross-reactivity Human, monkey Human, monkey
Specificity Claudin-5
No cross-reaction to Claudin-1-4,6,71)
Claudin-5
No cross-reaction to Claudin-1-4,6,71)
Concentration Indicated to the label
First lot:1.1mg/mL
Indicated to the label
First lot:1.2mg/mL
Application Flow cytometry
0.5-5μg/mL

Neutralizing assay(in vitro)
90-150μg/mL
Flow cytometry
0.5-5μg/mL

Neutralizing assay(in vitro)
90-150μg/mL

Application Data

Neutralizing assay in vitro BBB cell model

The barrier activity was evaluated by adding these antibodies (R9 and M48) were added to the blood-brain barrier cynomolgus cell model (BBB kit, PharmaCo-cell).

(1)Trans-epithelial/endothelial electrical resistance (TEER) (2) Substance permanent
04007766_img01.png 04007766_img02.png
These antibodies(R9 and M48) reduced TEER. The effects were higher than C-CPEmt.
 
These antibodies(R9 and M48) increased permanent of fluorescein isothiocyanate–labeled dextran(4kDa).The effects were higher than C-CPEmt.

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