Phos-tag™ Agarose Resins in Tip
Phos-tag™ Tip is a ready-to-use pipette-tip column prepacked with Phos-tag™ agarose. This column is a powerful tool for pretreating phosphorylated peptides.
The Phos-tag™ molecules in a pipette tip can specifically capture phosphorylated proteins in your samples. This allows for the easy purification of these proteins using only a pipetting operation.
- Short hands-on time (less than 30 min)
- High recovery yields
- No need for expensive equipment
Instructions for Use
Separation of Phosphopeptides from the Tryptic Digest of 6 nM β-Casein
- Following trypsin digestion of β-casein (6 nM), phosphorylated peptides were purified using a Phos-tag™ Tip column.
- Aliquots of the unpurified sample, flow-through fraction, and eluate were analyzed by HPLC.
We detected only phosphopeptide peaks (P1 and P2) in the eluate. This indicates that Phos-tag™ Tips can selectively trap and enrich phosphopeptides from the tryptic digest of β-casein.
- Open All
- Close All
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.