Fc fusion vectors
pCAG is a gene expression vector for mammalian cells. pCAG vector includes CAG promoter in itself. CAG promoter is known to be able to artificially induce high expression of target recombinant proteins in mammalian cells. pCAG-Hyg/Neo IgG-Fc contains each IgG Fc region including hinge sequence at C-terminal part of multiple cloning site (MCS) and expresses Fcfused proteins. Additionally, secretory signal is inserted into N-terminal part of MCS, so that Fc-fused chimeric proteins are secreted in a culture media※1.
Fc-fused chimeric proteins have functional properties derived from the original protein, and also have features such as a long half-life, increased expression of chimeric proteins in mammalian cell, and easy purification by protein A or G.
Thus, Fc-fused chimeric proteins have been used for functional analysis of proteins and basic study of recombinant antibody.
Features
- High expression by CAG promoter
- Optimized expression level of drug resistance gene
- Secretory signal is already inserted into N-terminal part of MCS
- Many restriction enzymes can be used in MCS
- License free ※2
Vector Map
※1: There is a possibility that Fc-fused chimeric proteins is not secreted in a conditioned medium. It depends on characters of a target protein.
※2: If reselling of the product, or sales of plasmids newly established using a DNA fragment or sequence derived from the product is intended, please contact our customer support office (ffwk-labchem-tec@fujifilm.com).
Restriction Enzyme List
| Vector | Acc I | BamH I | Cla I | EcoR I | EcoR V | Hind Ⅲ | Pst I | Sal I | Sma I | Spe I | Xho I |
|---|---|---|---|---|---|---|---|---|---|---|---|
| pCAG-Hyg hIgG1-Fc | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ||||
| pCAG-Hyg mIgG1-Fc | ○ | ○ | ○ | ○ | ○ | ○ | |||||
| pCAG-Hyg mIgG2a-Fc | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ||||
| pCAG-Hyg mIgG2b-Fc | ○ | ○ | ○ | ○ | ○ | ||||||
| pCAG-Hyg rIgG2a-Fc | ○ | ○ | ○ | ○ | ○ | ○ | |||||
| pCAG-Hyg rIgG2b-Fc | ○ | ○ | ○ | ○ | ○ | ||||||
| pCAG-Neo hIgG1-Fc | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | |
| pCAG-Neo mIgG1-Fc | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ||
| pCAG-Neo mIgG2a-Fc | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
| pCAG-Neo mIgG2b-Fc | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ||
| pCAG-Neo rIgG2a-Fc | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | |
| pCAG-Neo rIgG2b-Fc | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
1) Mean particle size is a middle one in the accumulated volume distribution.
2) 5% Dynamic Binding Capacity is measured by frontal analysis (Residence time 3 minutes).
Product List
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Human IgG1-Fc fusion vector
Mouse IgG1-Fc fusion vector
Mouse IgG2a-Fc fusion vector
Mouse IgG2b-Fc fusion vector
Rat IgG2a-Fc fusion vector
Rat IgG2b-Fc fusion vector
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.