Puromycin-Sensitive Aminopeptidase (PSA) Fluorescent Probe

• Synonym:：N-[4-(1,4-Dihydro-1-methyl-2,4-dioxo-3(2H)-quinazolinyl)-3,5-diethylphenyl]-5-(dimethylamino)-1-naphthalenesulfonamide
• Appearance: White to slightly brown, crystalline powder to powder
• Solubility: DMSO ＜10 mmol/L
• IC₅₀ for PSA: 4.6 μmol/L
  IC₅₀ for APN: >100 μmol/L
• λex：320 nm / λem：510 nm
  * Excitation wavelength and fluorescence wavelength are not optimized. Please change λex andλem according to your experimental condition and purpose.

MOLT-4 cells were treated with 10 μmol / L DAMPAQ-22 for 10 minutes.

Data provided by Dr. Yuichi Hashimoto, Institute of Molecular Cell Biology, University of Tokyo

1. Kakuta, H., Tanatani, A., Nagasawa, K. and Hashimoto, Y. : Chem. Pharm. Bull., 51, 1273(2003).
2. Kakuta, H., Koiso, Y., Nagasawa, K. and Hashimoto, Y. : Bioorg. Med. Chem. Lett., 13, 83(2003).
3. Bukowska, A., Tadje, J., Arndt, M., Wolke, C., Kahne, T., Bartsch, J., Faust, J., Neubert, K., Hashimoto, Y. and Lendeckel, U. : Biol. Chem., 384, 657(2003).
4. Sanchez-Moran, E., Jones, G.H., Franklin F.C. and Santos, J.L. : Plant Cell, 16, 2895(2004).
5. Thielitz, A., Bukowska, A., Wolke, C., Vetter, R., Lendeckel, U., Wrenger, S., Hashimoto, Y., Ansorge, S., Gollnick, H. and Reinhold, D. : Biochem. Biophys. Res. Commun., 321, 795(2004).
6. 和光純薬時報, 77(1), 2(2009).