Anti CTGF Antibodies
CTGF is a secreted protein of about 38 kDa produced from umbilical veins and vascular endothelial cells . CTGF is consist of four modules, and regulates chondrocyte proliferation / differentiation and intercellular adhesion. Some reports showed that CTGF is involved in various diseases such as cancer, renal failure, liver failure and rheumatism.
This items are mouse monoclonal antibodies established by DNA immunization, specifically react with native form CTGF. it has neutralizing activity.
Features
- CTGF Neutralizing Activity
Suppression of arthritis model animals (mice) - Each 4 clone recognizes
Each module 1, 2, 3 and 4, respectively
| Epitope | Clone No. | Cross- reactivity | Subclass | Applications | Concentration(1st lot) |
|---|---|---|---|---|---|
| Module1 | 30D2 | Human, Mouse | Mouse IgG1 | in vitro neutralization in vivo neutralization ELISA |
0.8 mg/mL |
| Module2 | 2-3 | Human, Mouse | Mouse IgG1 | in vitro neutralization | 0.9 mg/mL |
| Module3 | 3-54 | Human, Mouse | Mouse IgG1 | in vitro neutralization | 1 mg/mL |
| Module4 | 4-69 | Human, Mouse | Mouse IgG1 | in vitro neutralization | 0.7 mg/mL |
in vitro neutralization assay
Inhibition of osteoclastognenesis
Conditions
- PBMCs are collected from whole human blood and CD14+ cell is isolated.
- CD14+ cells are cultured for 7days (37℃, 5%CO2) with α-MEM (add 10% FBS) ,M-CSF （25ng/mL）, sRANKL （40ng/mL）and antiCTGF Abs.
- Osteoclasts (TRAP positive cells) were counted by optical microscopy.
Result
Enhancement of osteoclastogenesis by CTGF were repressed all CTGF antibodies.
This data was provided by Dr. Miyashita and Dr. Sekigawa of Institute for Environmental and Gender Specific Medicine, Juntendo University in Japan
in vivo neutralization assay
Suppression of arthritis model animals (mice)
Arthritis point criteria
| POINT | Observation |
|---|---|
| 0 | No change |
| 1 | Regional reddening of the limbs, or swelling and reddening of one finger. |
| 2 | Swelling of limbs' joints, or swelling of two or more fingers. |
| 3 | Medium - prominents swelling of hands and feet. |
| 4 | Remarkable swelling and redding of limbs, or hardening. |
※The above point is the total point of the limbs.
Conditions
DBA/1J mice (Female, 7-8 weeks of age) were immunized intradermally at the base of the tail with 100 μL of emulsion (in which emulsified in equal amounts of bovine type II collagen and Freund’s complete adjuvant (CFA) ).
The anti-CTGF antibody was intraperitoneally administered 1 week before the start of immunization, and administered once a week for a total of 8 times.
Evaluation of limb arthritis was performed once a week until immunization for 8 weeks.
(Refer to the table below for evaluation criteria)
Result
Mice treated with anti-CTGF module1 Ab significantly suppressed arthritis compared to the untreated mice with collagen induced arthritis.
This data was provided by Dr. Nozawa and Dr. Sekigawa of Institute for Environmental and Gender Specific Medicine, Juntendo University in Japan
References
- Ikawa ,Y., et al.: J. Cell Physiol., 216, 3, 680(2008).
- Nozawa, K., et al.: Arthritis Rheum., 65, 6, 1477 (2013).
- Miyashita, T., et al.: Autoimmunity., 49, 2,109 (2016).
- Miyazaki O, et al.: Ann. Clin. Biochem., 47, 205(2010).
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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