After cleavage of solid phase, deprotection of synthesized oligonucleotide is necessary to be carried out under mild conditions. In general, since amino group is protected by acyl group and cyano ethyl group is protecting group for hydroxy group of phosphoric acid, deprotections of them are carried out under basic conditions. And DMTr (dimethoxytrityl) is employed as a protecting group for 5'-OH of nucleotide so that acidic conditions are necessary for deprotecting process. Additionally, on the case of RNA synthesis, t-butyldimethylsilyl (TBDMS) group is used for hydroxy at 2'-position as protection. Deprotection for it is carried out using TBAF (tetrabutylammonium fluoride).
- Open All
- Close All
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.