Deprotection of 2' protecting group
For the purpose of RNA synthesis, the hydroxy group at the 2'-position of the sugar moiety is also protected. A tert-butyldimethylsilyl group (TBDMS group) or triisopropylsilyloxymethyl group (TOM group) is commonly used as the protecting group. Both TBDMS and TOM groups can be deprotected by treatment with fluoride ions such as tetrabutylammonium (TBAF) solution.
Protecting group at 2' position of the sugar moiety
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TBDMS group -
TOM group
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