TRAP/ALP Stain Kit

For enzyme activity staining of tartrate-resistant acid phosphatase and alkaline phosphatase

Normal bone metabolism is based on the balance between bone formation by osteoblasts and bone resorption by osteoclasts. When the balance is disturbed and bone resorption by osteoclasts is abnormally increased, bone mass is reduced leading to osteoporosis.
Therefore, various researches have been carried out to understand the mechanism of osteoclast and osteoblast metabolism, and to utilize this knowledge for the treatment of the diseases and for the development of effective drugs.
Today, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) are known as marker enzymes for osteoblasts and osteoclasts, respectively, and these enzymes are used as one of the markers showing the presence of osteoblasts and osteoclasts in tissue sections or cultured cells.
This kit enables you to examine the state of differentiation of bone cells and the cell distribution in bone tissues by observation of the stained images of osteoblasts and osteoclasts in the tissues and cultured cells using ALP/TRAP enzyme activities in the tissue sections and cultured cells.

[Features]

• In use, just mix two solutions for the preparation of the colorimetric substrate solution required for staining of acid phosphatase enzyme activity. (three solutions in case of the evaluation of tartrate-resistant acid phosphatase)


• Simple steps for staining of alkaline phosphatase enzyme activity by using ALP substrate soln. (premixed).


• Double staining can be performed, reddish-purple for active region of acid phosphatase and bluish brown for alkaline phosphatase.


• Usable in cultured cells and bone tissue sections (GMA resin embedded section).

[Kit Contents]

1. Sodium tartrate soln. (x10) ... 3 mL x 1 bottle


2. TRAP substrate soln. A ... 30 mL x 1 bottle


3. TRAP substrate soln. N ... 0.3 mL x 1 bottle


4. Nuclear stain soln. ... 10 mL x 1 bottle


5. ALP substrate soln. (premixed) ... 30 mL x 1 bottle