S. cerevisiae Direct Transformation Kit Wako

for Genetic Research

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at -20 degrees C.

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Comparison	Product Number	Package Size	Price	Inventory
	Distributor 296-62701 Barcode No 4987481447400	20tests	List Price 124.00 USD	In stock in Japan	Certificate of Analysis

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Overview / Applications

Outline	. This product is for research use only. Do not administer it to human. for genome-wide screening!! The S. cerevisiae Direct Transformation Kit Wako is specially designed for easy transformation of the budding yeast, Saccharomyces sp. The S. cerevisiae Direct Transformation Protocol allows successful transformation simply by mixing a plasmid DNA and the kit reagents with cultured yeast cells. No complicated steps, such as centrifugation or cell washing, are required. The S. cerevisiae Direct Transformation Kit Wako is particularly well suited for high-throughput transformationof a large number of yeast strains grown in 96-well plates. Even genome-wide screening, which has been problematic, becomes possible, since the S. cerevisiae Direct Transformation Kit Wako easily transforms about 4,850 strains with gene disruption. Features No centrifugation or washing steps are required. One-step procedure by mixing plasmid DNA and the kit reagents with cultured yeast cells. Suitable for transformation of a large number of yeast strains using 96-well plates. Applicable to tube protocol. Low-viscosity reagent enables high-throughput screening. Transformation Efficiency BY4743 strain with pRS316 plasmid 96-well plate protocol: 500 and over Tube protocol: 5,000 and over The product information is shown on the page #10 of Wako Product Update No.15.

Outline

.
This product is for research use only. Do not administer it to human.

for genome-wide screening!!

The S. cerevisiae Direct Transformation Kit Wako is specially designed for easy transformation of the budding yeast, Saccharomyces sp. The S. cerevisiae Direct Transformation Protocol allows successful transformation simply by mixing a plasmid DNA and the kit reagents with cultured yeast cells. No complicated steps, such as centrifugation or cell washing, are required. The S. cerevisiae Direct Transformation Kit Wako is particularly well suited for high-throughput transformationof a large number of yeast strains grown in 96-well plates. Even genome-wide screening, which has been problematic, becomes possible, since the S. cerevisiae Direct Transformation Kit Wako easily transforms about 4,850 strains with gene disruption.

Features

No centrifugation or washing steps are required.
One-step procedure by mixing plasmid DNA and the kit reagents with cultured yeast cells.
Suitable for transformation of a large number of yeast strains using 96-well plates.
Applicable to tube protocol.
Low-viscosity reagent enables high-throughput screening.

Transformation Efficiency BY4743 strain with pRS316 plasmid

96-well plate protocol: 500 and over
Tube protocol: 5,000 and over

The product information is shown on the page #10 of Wako Product Update No.15.

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

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