0.1mol/L Tris-HCl Buffer Solution (pH 7.3)

for Endotoxin Detection

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at 2-10 degrees C.

Structural Formula
Label
Packing

SDS

Comparison	Product Number	Package Size	Price	Inventory
	Distributor 201-10421 Barcode No 4987481342460	5mL x10	List Price 143.00 USD	In stock in Japan

Inquiry

Document
Overview / Applications
Property
Manufacturer Information
Alias

Document

SDS

Product Specification Sheet

Certificate of Analysis

Calibration Certificate

Overview / Applications

Outline	<Endotoxin, beta-Glucan & Peptidoglycan Detection Reagents><Reagent for Limulus Test> Endotoxin is a component of the outer membrane of Gram-negative bacteria and is a lipopolysaccharide (LPS). Since endotoxin causes serious symptoms such as fever and shock in the human body, the bacterial endotoxin test for pharmaceuticals, especially parenteral drugs, is important. Since J. Levin and F. B. Bang discovered that endotoxin gels an extract from horseshoe crab amebocytes (Limulus amebocyte lysate, or LAL), bacterial endotoxin detection by the Limulus test has been widely used. This method, known as the limulus test, was adopted in the US Pharmacopoeia in 1980, and later in 1988 in the Japanese Pharmacopoeia as well. <Related Reagent, Instrument><Designated Buffer Solution> Gelation reaction of the LAL reagent is known to be influenced very much by pH. The optimum pH for LAL gelation test is pH 6 to 7.5, and pH 7.3 using a toxinometer. In case the pH of the sample is outside this range, the pH must be adjusted with endotoxin-free sodium hydroxide solution or hydrochloric acid solution. The product is a buffer solution already adjusted, to be used with samples such as a drug which may cause changes in pH levels, in order to omit the above process of adjustment. When the LAL reagent is dissolved with the product instead of distilled water, the reagent will have a buffer action. pH 7.3 at 30 degrees centigrade

Outline

<Endotoxin, beta-Glucan & Peptidoglycan Detection Reagents><Reagent for Limulus Test>
Endotoxin is a component of the outer membrane of Gram-negative bacteria and is a lipopolysaccharide (LPS). Since endotoxin causes serious symptoms such as fever and shock in the human body, the bacterial endotoxin test for pharmaceuticals, especially parenteral drugs, is important. Since J. Levin and F. B. Bang discovered that endotoxin gels an extract from horseshoe crab amebocytes (Limulus amebocyte lysate, or LAL), bacterial endotoxin detection by the Limulus test has been widely used.
This method, known as the limulus test, was adopted in the US Pharmacopoeia in 1980, and later in 1988 in the Japanese Pharmacopoeia as well.
<Related Reagent, Instrument><Designated Buffer Solution>
Gelation reaction of the LAL reagent is known to be influenced very much by pH. The optimum pH for LAL gelation test is pH 6 to 7.5, and pH 7.3 using a toxinometer. In case the pH of the sample is outside this range, the pH must be adjusted with endotoxin-free sodium hydroxide solution or hydrochloric acid solution.
The product is a buffer solution already adjusted, to be used with samples such as a drug which may cause changes in pH levels, in order to omit the above process of adjustment.
When the LAL reagent is dissolved with the product instead of distilled water, the reagent will have a buffer action.
pH 7.3 at 30 degrees centigrade

Property

Appearance	Colorless clear liquid
pH	pH (30 degrees C) : 7.2 - 7.4
Flash Point	None

Manufacturer Information

Alias

0.1M TRIS-HCL BUFFER SOLN.

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.