Nuclease P1
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- CAS RN® :
- 54576-84-0
- Structural Formula
- Label
- Packing
- SDS
Comparison
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Product Number
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Package Size
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Inventory
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500U
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In stock |
Document
Overview / Applications
Outline | Nuclease P1 decomposes the 3'-5'-phosphodiester bond of RNA and heat denatured DNA. It also decomposes the 3'-phosphomonoester bond of mono-and oligonucleotides.In the presence of sodium chloride at 400 mmol/L or higher (pH 6.0), in particular, it does not affect double-stranded DNA. Usage example A. Sample : 200uL of RNA or heat-denatured DNA at a density of 2mg/mL. B. Reaction Buffer : 200uL of 0.01 mol/L barbital(veronal) acetate buffer, pH5.3*. C. Enzyme : 100uL of 100 units/mL of Nucelase P1 solution with distilled water. D. Stop Solution : 500uL of cold uranyl acetate buffer(0.25% of uranyl acetate and 2.5% perchloric acid) *barbital(veronal) acetate buffer, pH5.3 : Prepare 0.01mol/l Barbital Sodium Salt in water and adjust it to pH 5.3 with acetic acid. 1. Mix each 200uL of A, B and C. 2. Incubate it at 50 degree C for 1 hour. 3. The reaction can be stopped by adding 500uL of D. Reference Masao Fujimoto, Akira Kuninaka & Hiroshi Yoshino(1974)Purification of a Nuclease from Penicillium citrinum, Agr. Biol. Chem.,38(4), 777-783. |
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Property
Appearance | Lyophilisate |
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Origin / Source | Penicillium citrinum |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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