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Nuclease P1

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
CAS RN® :
54576-84-0
  • Structural Formula
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SDS
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Product Number
Package Size
Price
Inventory
Distributor
145-08221
Barcode No
4987481447172
500U
List Price
305.00 USD

In stock

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview / Applications

Outline Nuclease P1 decomposes the 3'-5'-phosphodiester bond of RNA and heat denatured DNA. It also decomposes the 3'-phosphomonoester bond of mono-and oligonucleotides.In the presence of sodium chloride at 400 mmol/L or higher (pH 6.0), in particular, it does not affect double-stranded DNA.

Usage example
A. Sample : 200uL of RNA or heat-denatured DNA at a density of 2mg/mL.
B. Reaction Buffer : 200uL of 0.01 mol/L barbital(veronal) acetate buffer, pH5.3*.
C. Enzyme : 100uL of 100 units/mL of Nucelase P1 solution with distilled water.
D. Stop Solution : 500uL of cold uranyl acetate buffer(0.25% of uranyl acetate and 2.5% perchloric acid)

*barbital(veronal) acetate buffer, pH5.3 : Prepare 0.01mol/l Barbital Sodium Salt in water and adjust it to pH 5.3 with acetic acid.

1. Mix each 200uL of A, B and C.
2. Incubate it at 50 degree C for 1 hour.
3. The reaction can be stopped by adding 500uL of D.

Reference
Masao Fujimoto, Akira Kuninaka & Hiroshi Yoshino(1974)Purification of a Nuclease from Penicillium citrinum, Agr. Biol. Chem.,38(4), 777-783.

Property

Appearance Lyophilisate
Origin / Source Penicillium citrinum

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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