Nitro Blue Tetrazolium Chloride

for Biochemistry

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at 2-10 degrees C.

CAS RN^® :: 298-83-9
Molecular Formula :: C40H30Cl2N10O6
Molecular Weight :: 817.64

GHS :

Citations (6)

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Structural Formula
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SDS

Product Number	Package Size	Price	Availability	Certificate of Analysis	Purchase
Distributor 144-01993 Barcode No 4987481300712	1G	List Price 140.00 USD	In stock in Japan	Certificate of Analysis	Distributor
Distributor 142-01994 Barcode No 4987481300729	5G	List Price 432.00 USD	In stock in Japan	Certificate of Analysis	Distributor
Distributor 148-01991 Barcode No 4987481300699	100mg		Discontinued	Certificate of Analysis	Distributor
Distributor 148-01996 Barcode No 4987481380578	250mg	List Price 55.00 USD	In stock in Japan	Certificate of Analysis	Distributor

※Check availability in the US with the distributor.

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Overview / Applications
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Product Specification Sheet

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Spectral Data

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Calibration Certificate

Analytical Charts

Overview / Applications

Outline	The product is tetrazolium blue into which a nitro group has been introduced to improve its stainability.It has a lower reduction potential (-0.05 V) than tetrazolium blue and is easily reduced in the presence of dehydrogenase to precipitate as blueish-purple diformazan (lambda max = 530 nm, epsilon = 3.6x10^4), so it may be used for detecting and determining dehydrogenases. It is a representative tetrazolium salt, because it is also superior as a stain used in the LDH fractionation method combined with agar electrophoresis and zymography.Detectable substances: Reagents for detection and determination include LDH, SDH, TPN, DPN, glutaminaseReagents for staining in electrophoresis include LDH, etc.Reagents for detection by electron microscopy include LDH, etc.An antibody for a target substance to be searched is prepared, the antibody or the secondary antibody is enzyme-labeled and reacted with the target substance in tissues and cells, the enzyme is reacted with a substrate and stained, followed by microscopical observation.Used as a substrate for glucoseoxidase.

Outline

The product is tetrazolium blue into which a nitro group has been introduced to improve its stainability.It has a lower reduction potential (-0.05 V) than tetrazolium blue and is easily reduced in the presence of dehydrogenase to precipitate as blueish-purple diformazan (lambda max = 530 nm, epsilon = 3.6x10^4), so it may be used for detecting and determining dehydrogenases. It is a representative tetrazolium salt, because it is also superior as a stain used in the LDH fractionation method combined with agar electrophoresis and zymography.Detectable substances: Reagents for detection and determination include LDH, SDH, TPN, DPN, glutaminaseReagents for staining in electrophoresis include LDH, etc.Reagents for detection by electron microscopy include LDH, etc.An antibody for a target substance to be searched is prepared, the antibody or the secondary antibody is enzyme-labeled and reacted with the target substance in tissues and cells, the enzyme is reacted with a substrate and stained, followed by microscopical observation.Used as a substrate for glucoseoxidase.

Property

Appearance	Yellow, crystalline powder - powder
Melting Point	184 - 210 degrees C

Manufacturer Information

Alias

Same CAS list

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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