Formamide

for Biochemistry

Specification Assay :: 98.0+% (capillary GC)

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at -20 degrees C.

CAS RN^® :: 75-12-7
Molecular Formula :: HCONH2
Molecular Weight :: 45.04

GHS :

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	Distributor 066-02301 Barcode No 4987481267558	200mL	List Price 58.00 USD	In stock in Japan	Certificate of Analysis

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Product Specification Sheet

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Overview / Applications

Outline	Formamide weakens the intra-base action inside single-strand DNA to maintain the single-stranded part in extended state and lowers the melting point (Tm) of DNA in hybrid formation¹⁾. Therefore, it is used in the buffer solution for DNA denaturation and hybridization in the DNA base sequencing by the Maxam-Gilbert method and Sanger's method, etc. and is one of organic reagents used frequently in the genetic field.This product, which is deionized and has minimized conductivity, is most suited as a denaturing agent in the electrophoresis for nucleic acid base sequencing, etc. and hybridization.Conductivity: max. 3 x 10 ^ -5 S/cm
Precautions for Use	Packed on inactive gas

Outline

Formamide weakens the intra-base action inside single-strand DNA to maintain the single-stranded part in extended state and lowers the melting point (Tm) of DNA in hybrid formation¹⁾. Therefore, it is used in the buffer solution for DNA denaturation and hybridization in the DNA base sequencing by the Maxam-Gilbert method and Sanger's method, etc. and is one of organic reagents used frequently in the genetic field.This product, which is deionized and has minimized conductivity, is most suited as a denaturing agent in the electrophoresis for nucleic acid base sequencing, etc. and hybridization.Conductivity: max. 3 x 10 ^ -5 S/cm

Precautions for Use

Packed on inactive gas

Property

Appearance	Colorless clear liquid
Melting Point	2 - 3 degrees C
Freezing Point	1.5 - 3.0 degrees C
Boiling Point	about 210 degrees C
Flash Point	154 degrees C (tag closed test)
Specific Gravity	1.138 (20/20 degrees C)

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