Anti c-Myc, Monoclonal Antibody

for Immunochemistry

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at -20 degrees C.

Host :: Mouse
Clone No. :: 9E10

Classification :: Monoclonal

GHS :

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Structural Formula
Label
Packing

SDS

Product Number	Package Size	Price	Availability	Certificate of Analysis	Purchase
Distributor 011-21874 Barcode No 4548995013477	1mg	List Price 883.00 USD	In stock in Japan	Certificate of Analysis	Distributor
Distributor 017-21876 Barcode No 4548995013484	5mg		Discontinued	Certificate of Analysis	Distributor
Distributor 017-21871 Barcode No 4987481478022	200ug	List Price 359.00 USD	In stock in Japan	Certificate of Analysis	Distributor

※Check availability in the US with the distributor.

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Overview / Applications
Property
Antibody Information
Manufacturer Information
Alias
Related Information

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SDS

Product Specification Sheet

Package Insert

Certificate of Analysis

Calibration Certificate

Analytical Charts

Overview / Applications

Outline	This product is used for detection of c-Myc polypeptide, EQKLISEEDL, fusion recombinant protein by using immunoprecipitation and western blot. This product is for research use only. Do not administer it to human. c-Myc is a proto-oncogene, which is overexpressed in a wide range of human cancers. A proto-oncogene is a normal gene that can become an oncogene due to mutation or increase of expression. The partial peptide of c-Myc protein which consists of 10 amino acids, EQKLISEEDL, has been used as the tag for detection and/or purification of recombinant protein. Monoclonal Anti c-Myc antibody was produced by hybridoma clone 9E10. This clone was established by fusing mouse myeloma cells and lymphocytes of mice immunized with synthetic EQKLISEEDL peptide which was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites. [Concentration] Indicated on the label. [Formulation] 1×PBS aqueous solution with 50% glycerol. [Clone No.] 9E10 [Subclass] IgG₁ [Specificity] Specific for the EQKLISEEDL peptide of c-Myc protein. [Working Concentration] Western blot 0.2 - 2 µg/mL Immunoprecipitation 1 - 2 µg/20 µL Protein A or Protein G beads slurry
Instructions	Western Blot(0.5ug/mL), Immunoprecipitation(2ug/300mL cell lysis solution).

Outline

This product is used for detection of c-Myc polypeptide, EQKLISEEDL, fusion recombinant protein by using immunoprecipitation and western blot.
This product is for research use only. Do not administer it to human.

c-Myc is a proto-oncogene, which is overexpressed in a wide range of human cancers. A proto-oncogene is a normal gene that can become an oncogene due to mutation or increase of expression.
The partial peptide of c-Myc protein which consists of 10 amino acids, EQKLISEEDL, has been used as the tag for detection and/or purification of recombinant protein.
Monoclonal Anti c-Myc antibody was produced by hybridoma clone 9E10. This clone was established by fusing mouse myeloma cells and lymphocytes of mice immunized with synthetic EQKLISEEDL peptide which was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites.

[Concentration]
Indicated on the label.

[Formulation]
1×PBS aqueous solution with 50% glycerol.

[Clone No.]
9E10

[Subclass]
IgG₁

[Specificity]
Specific for the EQKLISEEDL peptide of c-Myc protein.

[Working Concentration]
Western blot 0.2 - 2 µg/mL
Immunoprecipitation 1 - 2 µg/20 µL Protein A or Protein G beads slurry

Instructions

Western Blot(0.5ug/mL), Immunoprecipitation(2ug/300mL cell lysis solution).

Property

Appearance	Liquid
Concentration	0.4 - 0.6mg/mlProtein

Antibody Information

Antigen	c-Myc
Antigen Alias	C-Myc V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) V-Myc Myelocytomatosis Viral Oncogene Homolog alpha-c-Myc bHLHe39 C-myc peptide Myc v-myc myelocytomatosis viral oncogene homolog (avian)

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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