Anti AGEs, Monoclonal Antibody (clone:6D12)

Package Size: 10 mg/vial (40 mL/vial)Form: Anti AGEs-mouse monoclonal antibody 0.25 mg/mL (in PBS,pH 7.2)

Manufactured by Trans Genic Inc. (Kumamoto, Japan)
(AGEs: Advanced glycation end products)
Clone No.: 6D12
Subclass: IgG1
Purification method: The splenic lymphocytes from BALB/c mice immunized with AGEs-BSA were fused to myeloma P3U1 cells. The hybrid cells were screened, and the cell line (6D12) with positive reaction to AGEs-human serum albumin but negative to BSA was selected through successive subclonings and grown in ascetic fluid of BALB/c mice, from which the anti-AGEs antibody was purified by Protein A affinity chromatography.
Working dilution for Immunohistochemistry: 2 mg/mL, for ELISA: 0.1 - 0.5 mg/mL
Specificity:
Positive reaction to 1 - 5
1. Advanced stage of the Maillard reaction
2. AGEs-protein (AGEs-BSA, -HSA, -Hb, -Collagen)
3. AGEs-poly-Lys
4. AGEs-Lys derivatives (AGEs-a-Tos-Lys,-a-Tos-Lys-o-Me)
5. AGEs-mono amino carboxylic acids (AGEs-b-alanine, AGEs-g-aminobutyric acid, AGEs-e-aminocaproic acid)
Negative reaction to 6 - 11
6. Early stage of the Maillard reaction (Shiff base adducts and Amadori rearrangement products)
7. Unmodified protein (BSA, HSA, Hb, Collagen)
8. Unmodified poly-Lys
9. Unmodified Lys derivatives (a-Tos-Lys, a-Tos-Lys-o-Me)
10. Unmodified mono amino carboxylic acids (b-alanine, g-aminobutyric acid, and e-aminocaproicacid)
11. FFI, Pyrrole aldehyde, Pentosidine