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LBIS Mouse anti-dsDNA ELISA Kit

Manufacturer :
FUJIFILM Wako Shibayagi Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
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Product Number
Package Size
Price
Inventory
Distributor
637-02691
Manufacturer
AKRDD-061
96tests
List Price
735.00 USD

In stock in Japan

Document

Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview

Features

  • Measurable in a short period
  • A minute quantity of sample is measurable
  • Eco-friendly preservatives are used
  • All reagents are solution types and are ready to use
  • High measurement accuracy and reproducibility

Product Information

Sample: serum or plasma

Assay range

Accuracy test

• Do not use heparin as anticoagulant.
• Do not use heat-inactivated samples.

15.6~1,000 mU/mL

Precision of assay : Mean CV<10%
Reproducibility : Mean CV<10%

Mouse sample measurement result

00046539_img02.png

Changes in autoantibody titers over time in MRL/Ipr mice
Japanese journal of inflammation VOl.20 No.6, Nov. 2000

Autoantibody titers in serum of MRL/lpr mice (n = 7) were measured over time. In the clinical trial, antibody titers of IgG-RF antibody, IgM-RF antibody, and anti ssDNA were significantly inhibited compared to a 20-week-old MRL/lpr mouse (*p<0.05)

References

    1. The ERM Protein Moesin Regulates CD8+ Regulatory T Cell Homeostasis and Self-Tolerance.
      Satooka H, Nagakubo D, Sato T, Hirata T.
      J Immunol. 2017 Oct 4. pii: ji1700074.
    2. JAK inhibitor has the amelioration effect in lupus-prone mice: the involvement of IFN signature gene downregulation.
      Ikeda K, Hayakawa K, Fujishiro M, Kawasaki M, Hirai T, Tsushima H, Miyashita T, Suzuki S, Morimoto S, Tamura N, Takamori K, Ogawa H, Sekigawa I.
      BMC Immunol. 2017 Aug 22;18(1):41.
    3. Flagellar filament structural protein induces Sjögren’s syndrome-like sialadenitis in mice.
      Higuchi T, Haruta I, Shibata N, Yanagisawa N, Yagi J
      Oral Dis. 2017 Jul;23(5):636-643.

Overview / Applications

Outline LBIS Mouse anti-dsDNA ELISA Kit is a sandwich ELISA system for quantitative measurement of mouse anti-dsDNA antibody titer. This is intended for research use only.

[Assay Range]
15.6 -1,000 mU/mL
[Test Time]
4hours20minutes
[Sample Volume]
100uL(diluted samples)
[Samples]
Mouse Serum,Plasma
Features 1)In 4 - 5 hours, mouse anti-dsDNA is quantitated.
2) Excellent precision and reproducibility.
3)Simple assay procedure
4)Enable us to detect mouse anti-dsDNA in a small quantity of sample
Kit Components (A) dsDNA-coated 96 well-plate Use after washing 96 wells/1 plate
(B) Mouse anti-dsDNA standard
(10000 mU/mL)*(derived from mouse)
*The value is different depending on the lot.
Concentrated. Use after dilution. 100 uL/1 vial
(C) Buffer solution Ready for use. 60 mL/1 bottle
(D) HRP-labeled anti-mouse IgG antibody Concentrated. Use after dilution. 20 uL/1 vial
(E) Chromogen(TMB) Ready for use. 12 mL/1 bottle
(F) Stop solution (1M H2SO4) Be careful! Ready for use. 12 mL/1 bottle
(G) Wash stock solution (10x) Concentrated. Use after dilution. 100 mL/1 bottle
Plate seal 3 sheets
Instruction Manual 1 copy

Property

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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