Events / Exhibitions

Outline

Date

8th - 12th May, 2024

Place

Melbourne Convention and Exhibition Center in Melbourne, Australia

Address

1 Convention Centre Pl, South Wharf VIC 3006, Australia

Booth#

19

Opening Hours

8:30 AM - 5:35 PM

Registration

Click HERE

　

Showcase

Date

Saturday, May 11, 2024

Time

12:05 - 12:50 p.m.

Room

Courtyard

Speaker

Koji Ueda, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research

Title

In-depth proteome profiling of EVs for quality assessment and liquid biopsy development

Note

No pre-registration required. Maximum 70 people (first-come-first-served basis)

　

Summary of the showcase

Title: In-depth proteome profiling of EVs for quality assessment and liquid biopsy development

Numerous studies on EVs in recent years have led to great interest not only in elucidating their basic biological functions, such as biosynthetic pathways and functions, but also as diagnostic and therapeutic tools. In fact, liquid biopsy targeting EV-encapsulated molecules and therapies using MSC-derived EVs are approaching practical application. On the other hand, the key to any EV research is the purity of the EVs to be handled. For example, it is not difficult to imagine that the purity of the EVs will greatly affect the results of biomarker discovery based on omics analyses of plasma-derived EVs or those of MSC-derived EV therapies. In this presentation, we will show the results of our EV enrichment score (EES) evaluation method based on in-depth proteome analysis and the evaluation results of various EV purification techniques. Based on these assessments, we will also show the results of our research for the development of biomarkers for cancer or dementia using EVs purified by the MagCapture Exosome Isolation Kit PS Ver. 2 (FUJIFILM Wako Pure Chemical Corporation) on the 96-well processing robot. We will also introduce the MassivEV EV Purification Column PS for mass production of therapeutic EVs and EV-Save reagent that minimizes adsorption loss during storage. These tools are expected to further accelerate basic research and clinical applications of EVs.

In this showcase you'll learn

• Isolation method of Extracellular Vesicles with high purity and less damage

Who should attend

• Extracellular Vesicle researchers

　

Related product

The MassivEV™ EV Purification Column PS and MassivEV™ Purification Buffer Set have been specifically designed for the isolation and purification of extracellular vesicles (EVs), including exosomes, from large volumes of cell culture supernatants. This is achieved by utilizing the PS affinity method, which employs the Tim4 protein. Tim4 binds to phosphatidylserine (PS) on the surface of EV membranes in a calcium-dependent manner, making the purification of high-purity EVs a straightforward exercise.

Features

• High-purity EVs can be efficiently isolated and purified from large volumes of cell culture supernatants (from 10 mL to several liters)
• Eliminates the need for expensive equipment like tangential flow filtration (TFF) systems.* Only a peristaltic pump is required.