LabAssay™ Creatinine
- for Cellbiology
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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500Tests
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In stock in Japan |
Document
Kit component
Kit component
| Deproteinizing Reagent | 150 mL x 1 bottle |
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| Picric Acid Reagent (Picric Acid 22 mmol/L) | 50 mL x 1 bottle |
| 0.75 mol/L Sodium Hydroxide Solution | 50 mL x 1 bottle |
| Standard Solution (Creatinine 10 mg/dL) | 15 mL x 1 bottle |
Overview
Introduction
Creatinine is a metabolite which is produced by creatine phosphate directly or dehydration of creatine in muscle and nerve. It is excreted from the body through kidney glomerular filtration.
Assay principle
LabAssay™ Creatinine can be used to measure the creatinin levels in samples by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline condition.
Performance
| Sample | Serum, Plasma, Urine |
|---|---|
| Target animals | Human, Mouse, Rat, Cat, Dog |
| Standard curve range | 2.5 - 10mg/mL (mg/100 mL) |
| Measurement time | Approx. 40 min. |
| Amount of sample | 50 μL |
| Measurement wavelength | 520 nm |
Standard Curve
Procedure
Perform the assay in the wells according to the following table scheme.
| Test | Standard | Blank | |
|---|---|---|---|
| First operations should be performed in the sample tubes. | |||
| Sample | Sample 50 μL |
Standard solution 50 μL |
Water 50 μL |
| Deproteinizing reagent | 300 μL | 300 μL | 300 μL |
| Mix well, and stand at room temperature for 10 min. Centrifuge over 2500 rpm for 10 min. Subsequent operations should be performed in the wells. |
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| Supernatant | 100 μL | 100 μL | 100 μL |
| Stand at 25 ℃ - 30 ℃ | |||
| Picric Acid Reagent | 50 μL | 50 μL | 50 μL |
| 0.75 mol/L Sodium Hydroxide Solution | 50 μL | 50 μL | 50 μL |
| Stand at 25 ℃ - 30 ℃ for 20 min. Measure the absorbance of the test sample and standard solution at 520nm with the blank solution as the control within the next 30 min. |
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Data
Spike-and-Recovery Test
Spike and Recovery Test of Creatinine, a known amount of standard is spiked into in human,mouse,rat sample n=2.
| Human Sample | Spike (mg/dL) | Mean (mg/dL) | Recovery (mg/dL) | Recovery (%) |
|---|---|---|---|---|
| Serum | - | 3.68 | - | - |
| 2.71 | 6.37 | 2.70 | 99.6 | |
| 3.55 | 7.10 | 3.42 | 96.3 | |
| 4.21 | 7.55 | 3.87 | 91.9 | |
| Plasma (EDTA) |
- | 3.43 | - | - |
| 3.30 | 6.47 | 3.04 | 95.2 | |
| 4.33 | 7.39 | 3.96 | 91.5 | |
| 5.13 | 7.69 | 4.26 | 83.0 | |
| Plasma (Heparin) |
- | 3.37 | - | - |
| 3.31 | 6.97 | 3.60 | 109 | |
| 4.34 | 7.86 | 4.49 | 103 | |
| 5.14 | 8.21 | 4.84 | 94.2 |
| Rat Sample | Spike (mg/dL) | Mean (mg/dL) | Recovery (mg/dL) | Recovery (%) |
|---|---|---|---|---|
| Serum | - | 2.86 | - | - |
| 3.27 | 5.61 | 2.75 | 84.1 | |
| 5.60 | 8.13 | 5.27 | 94.1 | |
| 7.35 | 9.34 | 6.48 | 88.2 | |
| Plasma (EDTA) |
- | 2.78 | - | - |
| 2.79 | 5.22 | 2.44 | 87.5 | |
| 4.78 | 8.3 | 5.52 | 115 | |
| 6.27 | 9.22 | 6.44 | 103 | |
| Plasma (Heparin) |
- | 2.88 | - | - |
| 3.17 | 5.53 | 2.65 | 83.6 | |
| 5.43 | 8.25 | 5.37 | 98.9 | |
| 7.13 | 9.27 | 6.39 | 89.6 |
| Mouse Sample | Spike (mg/dL) | Mean (mg/dL) | Recovery (mg/dL) | Recovery (%) |
|---|---|---|---|---|
| Serum | - | 2.68 | - | - |
| 3.00 | 5.90 | 3.22 | 107 | |
| 5.14 | 8.08 | 5.40 | 105 | |
| 6.74 | 8.90 | 6.22 | 92.3 | |
| Plasma (EDTA) |
- | 3.14 | - | - |
| 2.71 | 5.63 | 2.49 | 91.9 | |
| 4.65 | 7.74 | 4.60 | 98.9 | |
| 6.10 | 8.25 | 5.11 | 83.8 | |
| Plasma (Heparin) |
- | 3.01 | - | - |
| 2.85 | 5.48 | 2.47 | 86.7 | |
| 4.88 | 7.74 | 4.73 | 96.9 | |
| 6.41 | 8.60 | 5.59 | 87.2 |
Linearity-of-Dilution Test
- Human sample
- Rat sample
- Mouse sample
Example of measurement
| Human serum, pooled | |||||
|---|---|---|---|---|---|
| No. | Measured(mg/dL) | Mean(mmol/L) | SD | CV(%) | |
| 1 | 3.69 | 3.67 | 3.68 | 0.012 | 0.33 |
| Human plasma EDTA, pooled | |||||
| No. | Measured(mg/dL) | Mean(mmol/L) | SD | CV(%) | |
| 1 | 3.43 | 3.44 | 3.43 | 0.0071 | 0.21 |
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Normal Rat serum, Sprague Dawley, male No. Measured(mg/dL) mean SD CV(%) 1 0.553 0.546 0.550 0.0049 0.90 2 0.716 0.824 0.770 0.076 9.9 3 1.12 1.26 1.19 0.095 8.0 4 1.22 1.29 1.25 0.050 4.0 5 1.04 0.994 1.01 0.029 2.9 -
Normal Rat plasma K2EDTA, Sprague Dawley, male No. Measured(mg/dL) mean SD CV(%) 1 0.319 0.288 0.304 0.022 7.2 2 0.638 0.627 0.633 0.0078 1.2 3 0.824 0.871 0.848 0.033 3.9 4 0.661 0.681 0.671 0.014 2.1 5 0.205 0.233 0.219 0.020 9.0
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Normal Mouse serum, CD-1(ICR), male No. Measured(mg/dL) mean SD CV(%) 1 0.199 0.205 0.202 0.0042 2.1 2 0.492 0.507 0.500 0.011 2.1 3 0.734 0.722 0.728 0.0085 1.2 4 0.641 0.716 0.679 0.053 7.8 5 0.192 0.219 0.206 0.019 9.3 -
Normal Mouse plasma K2EDTA, CD-1(ICR), male No. Measured(mg/dL) mean SD CV(%) 1 0.0950 0.103 0.0990 0.0057 5.7 2 0.607 0.559 0.583 0.034 5.8 3 0.556 0.528 0.542 0.020 3.7 4 0.490 0.546 0.518 0.040 7.6 5 0.113 0.110 0.112 0.0021 1.9
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Normal Canine serum, Beagle, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 1.67 1.48 1.57 0.13 8.4 4 2 1.16 1.07 1.11 0.063 5.7 6 3 1.54 1.41 1.48 0.092 6.2 6 4 1.58 1.40 1.49 0.13 8.9 11 5 1.46 1.49 1.47 0.023 1.6 12 6 1.17 1.20 1.19 0.027 2.3 6 7 1.16 1.20 1.18 0.027 2.3 10 8 1.23 1.30 1.27 0.047 3.7 4 -
Normal Canine plasma EDTA, Beagle, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 1.48 1.45 1.46 0.021 1.5 4 2 1.21 1.18 1.20 0.024 2.0 6 3 1.33 1.22 1.27 0.071 5.6 10 4 1.56 1.49 1.53 0.048 3.2 12 5 1.31 1.29 1.30 0.0099 0.76 6 6 1.04 1.04 1.04 0.0028 0.27 11 7 1.14 1.16 1.15 0.013 1.2 10 8 1.26 1.29 1.28 0.019 1.5 12
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Normal Feline serum, Short hair, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 1.86 1.78 1.82 0.060 3.3 3.0 2 1.58 1.53 1.55 0.034 2.2 3.0 3 1.81 1.68 1.75 0.092 5.3 6.0 4 2.17 1.95 2.06 0.16 7.6 4.0 5 1.88 1.92 1.90 0.030 1.6 6.0 6 1.89 1.86 1.87 0.017 0.91 6.0 7 1.71 1.76 1.73 0.040 2.3 1.0 8 1.48 1.56 1.52 0.055 3.6 1.0 -
Normal Feline plasma EDTA, Short hair, male No. Measured(mg/dL) mean SD CV(%) Age(years) 1 1.63 1.61 1.62 0.011 0.65 0.5 2 1.41 1.36 1.39 0.038 2.8 1.0 3 1.78 1.62 1.70 0.11 6.7 3.0 4 1.97 1.76 1.87 0.15 7.9 0.5 5 1.66 1.67 1.66 0.0085 0.51 3.0 6 1.28 1.29 1.29 0.0028 0.22 3.0 7 1.26 1.30 1.28 0.029 2.3 0.5 8 1.60 1.71 1.65 0.080 4.8 1.0
References
- Kawamoto, T. et al. : Glycative Stress Res., 3, 15 (2016). Relationships between urinary melatonin metabolites and glycative stress and body functional age *Human urine
- Guan, Y. et al. : J. Pharmacol. Sci., 135, 81 (2017). A protease-activated receptor-1 antagonist protects against podocyte injury in a mouse model of nephropathy *Mouse urine and plasma
- Ito, K. et al. : Biol. Pharm. Bull., 38, 1169 (2015). Increased Milk Protein Concentration in a Rehydration Drink Enhances Fluid Retention Caused by Water Reabsorption in Rats *Rat urine and plasma
- Tahara, Y. et al. : Med. Chem. commun., 8, 415 (2017). Encapsulation of a nitric oxide donor into a liposome to boost the enhanced permeation and retention (EPR) effect *Mouse Serum
- Nasrin, S. et al. : J. Pharmacol. Sci., 122, 270 (2013). Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis *Rat urine
- Toyama, K. et al. : Br. J. Pharmacol., 166, 1183 (2012). Loss of multidrug and toxin extrusion 1 (MATE1) is associated with metformin-induced lactic acidosis *Mouse plasma
Overview / Applications
| Outline | Creatinine is a metabolite which is produced by creatine phosphate directly or dehydration of creatine in muscle and nerve. It is excreted from the body through kidney glomerular filtration. This product can be used to measure the creatinin levels in samples by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline condition. |
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Property
Manufacturer Information
Alias
- 636-51011 (Previous code)
LABCREA-M1 (Previous code)
290-65901 (Previous code)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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