MagCapture(TM) microRNA Isolation Kit, Human Ago2

for Genetic Research

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at 2-10 degrees C.

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	Distributor 295-74001 Barcode No 4548995029874	10Tests	Inquire	Inquire	Certificate of Analysis

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Recovering microRNA from human cell strains
Recovering free Ago2-bound microRNA from human plasma
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Recovering microRNA from human cell strains

We used MagCapture™ microRNA Isolation Kit, Human Ago2 and a previous product to obtain microRNA from human K562 cells (1×10⁷ cells), then compared quantities of microRNA obtained with denaturing polyacrylamide gel electrophoresis (silver staining) (Fig. 1) and quantitative PCR (miR-92a quantity) (Fig. 2). Results showed that the two products yielded equivalent quantities of microRNA.

Recovering free Ago2-bound microRNA from human plasma

We used MagCapture™ microRNA Isolation Kit, Human Ago2 and a previous product to obtain microRNA from pooled plasma of healthy individuals, then compared quantities obtained with quantitative PCR (miR-92a quantity). As shown below, using the spin column method with the MagCapture™ resulted in approximately equivalent yield to our previous product, while the one-step method demonstrated greater quantities of microRNA recovered compared to our previous product.

Overview / Applications

Outline	This product is for research use only. Do not administer it to human. This kit utilizes immunoprecipitation methods using an anti human Ago2 antibody to easily prepare Ago2-bound microRNAs and their target mRNAs from human cells and tissue samples. In addition, Ago2-bound microRNAs present in the extracellular fluid such as plasma, serum, and cell culture supernatant samples can be also prepared in the same way. [Features] •High-efficient purification of microRNAs •Adoption of anti-human Ago2 antibody-immobilized magnetic beads •In a microRNA purification step, either the high-yield one-step method or the spin-column method yielding high-purity microRNAs is selectable •Setting of an optimized protocol each for samples of cultured cells and tissue, and samples of plasma, serum, and culture supernatant.

Outline

This product is for research use only. Do not administer it to human.

This kit utilizes immunoprecipitation methods using an anti human Ago2 antibody to easily prepare Ago2-bound microRNAs and their target mRNAs from human cells and tissue samples. In addition, Ago2-bound microRNAs present in the extracellular fluid such as plasma, serum, and cell culture supernatant samples can be also prepared in the same way.
[Features]
•High-efficient purification of microRNAs
•Adoption of anti-human Ago2 antibody-immobilized magnetic beads
•In a microRNA purification step, either the high-yield one-step method or the spin-column method yielding high-purity microRNAs is selectable
•Setting of an optimized protocol each for samples of cultured cells and tissue, and samples of plasma, serum, and culture supernatant.

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microRNA isolation
microRNA

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Product Name
MagCapture(TM) microRNA Isolation Kit, Mouse Ago2	for Genetic Research
microRNA ExtractorR SP Kit	for Genetic Research

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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