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microRNA ExtractorR SP Kit

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
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Distributor
295-71701
Barcode No
4987481622968
50Tests
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In stock in Japan

Document

Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit contents

Lysis Solution 20 mL x 1
Reducing reagent 60 μL x 1
Protease Solution 600 μL x 1
Enhancer 600 μL x 1
Washing Solution 1 12 mL x 1
Washing Solution 2 22.5 mL x 1
Elution Solution 4 mL x 1
Spin column/2 mL tube 50 sets

Overview

This kit is for extraction of microRNA from serum or plasma of humans and animals. It enables microRNA extraction without use of phenol, chloroform, and other hazardous reagents required in previous methods.

Protocol Flow

01537528_img01.png

Note

The following necessary reagents are not included.
1-Butanol (code No.022-16035)
2-Propanol (code No.168-21675)
Ethanol (code No.054-07225)

For plasma coagulation, please use EDTA or citric acid. Using heparin inhibit PCR detection after RNA extraction.

Application Data

Extraction from normal human plasma

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Higher extraction rates observed than from previous methods.

Extraction from rat plasma and serum

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Extraction from animal experiments possible.

Recovery rate and reproducibility

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High reproducibility exhibited.

Overview / Applications

Outline microRNA Extractor SP Kit is designed for the extraction of total RNA including microRNA from serum and plasma of human and rodents(rat, mouse etc.). The extraction method of this kit is based on the procedure using chaotropic reagent, which allows for a safe extraction without hazardous phenol/chloroform, and spin column. This kit is useful microRNA extraction kit for circulating microRNA in serum and plasma.

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Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

+49-2131-311-271

Reception hours: Mon-Fri 9:00 - 15:00 (CET)For other hours than the above, please contact us via the inquiry form.