ImmunoSaver

for Immunohistologic Staining

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep below 25 degrees C.

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Comparison	Product Number	Package Size	Price	Inventory
	Distributor 097-06192 Barcode No 4987481622555	25mL	Inquire	In stock in Japan	Certificate of Analysis

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Product Specification Sheet

Certificate of Analysis

25mL

Overview / Applications

Outline	This product is for research use only. Do not administer it to human. ImmunoSaver is an antigen retrieval reagent for formalin-fixed tissues. This product is useful for the retrieval of a variety of antigens, not only those obtained using traditional methods ^{1, 2)}. Formalin-fixed tissues are known to form a methylene bridge between proteins, masking the antigenic site and reducing antigenicity ³⁾. The antigenicity can be recovered by using this product.Formulation: Citraconic Acid Sodium is included. Procedure: Dilute ImmunoSaver 200 times with distilled water or deionized water. (Note) Do not dilute ImmunoSaver with buffersolution such as PBS. Heat to 98°C. Place the depraffinized tissue sections into the solution and incubate for 45 minutes at 98°C. Wash the deparaffinized tissue sections 3 times in PBS for 5 minutes. Perform immunostaining. References Nmimatsu, S., Ghaizadeh, M. and Sugisaki, Y.: J. Histochem. Cytochem., 53, 3 (2005) Alelú-Paz, R., Iturrieta-Zuazo, I. Byne, W., Haroutunian, V., García-Villanueva, M., Rábano, A., García-Amado, M., Prensa, L. and Giménez-Amaya, J. M.: PLoS ONE, 3, e3378 (2008). D'Amico, F., Skarmoutsou, E. and Stivala, F.: J. Immunol. Methods, 341, 1 (2009).

Outline

This product is for research use only. Do not administer it to human.

ImmunoSaver is an antigen retrieval reagent for formalin-fixed tissues.
This product is useful for the retrieval of a variety of antigens, not only those obtained using traditional methods ^{1, 2)}.
Formalin-fixed tissues are known to form a methylene bridge between proteins, masking the antigenic site and reducing antigenicity ³⁾. The antigenicity can be recovered by using this product.Formulation: Citraconic Acid Sodium is included.
Procedure:

Dilute ImmunoSaver 200 times with distilled water or deionized water. (Note) Do not dilute ImmunoSaver with buffersolution such as PBS.
Heat to 98°C.
Place the depraffinized tissue sections into the solution and incubate for 45 minutes at 98°C.
Wash the deparaffinized tissue sections 3 times in PBS for 5 minutes.
Perform immunostaining.

References

Nmimatsu, S., Ghaizadeh, M. and Sugisaki, Y.: J. Histochem. Cytochem., 53, 3 (2005)
Alelú-Paz, R., Iturrieta-Zuazo, I. Byne, W., Haroutunian, V., García-Villanueva, M., Rábano, A., García-Amado, M., Prensa, L. and Giménez-Amaya, J. M.: PLoS ONE, 3, e3378 (2008).
D'Amico, F., Skarmoutsou, E. and Stivala, F.: J. Immunol. Methods, 341, 1 (2009).

Property

pH	6.9 - 7.8 (25 degrees C)
Density	1.0 - 1.2g/mL (20 degrees C)

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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ImmunoSaver

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