Fibronectin, Human, recombinant

Fibronectin is a glycoprotein widely distributed throughout the extracellular matrix (ECM). It is involved in various biological processes, including cell adhesion, proliferation, differentiation, and migration. Composed of a dimer of approximately 220 kDa subunits, it specifically binds to diverse molecules—such as integrin receptors on the cell surface and ECM components including collagen, fibrin, complement proteins, glycosaminoglycans, and heparin—via its multiple functional domains.

In vivo, fibronectin is produced by mesenchymal cells such as fibroblasts, chondrocytes, macrophages, and hepatocytes, playing a crucial role in maintaining tissue architecture and promoting injury repair. Particularly at wound sites, fibronectin rapidly deposits to form cell attachment sites, which facilitates the migration and proliferation of fibroblasts and vascular endothelial cells to accelerate tissue repair. Additionally, it is stored in platelet alpha-granules and expressed on the platelet surface upon activation, contributing to the hemostatic mechanism.

In the fields of tissue engineering and regenerative medicine, fibronectin is utilized as a matrix for cell culture. Biomaterials containing fibronectin enhance cell attachment and promote 3D tissue formation, supporting biocompatibility and functional tissue reconstruction. As a result, it is expected to improve cell culture efficiency and post-transplantation engraftment rates, leading to various advanced applications.

• Purified using a high-purity, agarose-based matrix free of animal-derived substances
• Exhibits cell adhesion activity equivalent to or higher than native fibronectin
• Eliminates the risk of pathogen contamination as it is not derived from blood

• Lyophilized
• Bacterial endotoxin tested
• Protein concentration: 450–550 μg/mL
• Sterility tested
• Mycoplasma tested

【Modular Structure】

Preparation

Add 2 mL of sterile purified water to the vial and let it stand at room temperature for 30 minutes.
(The resulting fibronectin concentration after reconstitution is 0.5 mg/mL.)

Storage

For storage at -20℃ or lower, aliquot into smaller portions to avoid repeated freeze-thaw cycles.
Stable for approximately 6 months under proper storage conditions.

Coating Protocol (Example)

1. Dilute the prepared solution with PBS(-) or a similar buffer, and coat the plate at a concentration of 1-5 µg/cm².
   Example: To coat a 6-well plate (9.6 cm²/well) at 2.5 µg/cm² (24 µg/well), add 2 mL of fibronectin solution
   diluted to 12 µg/mL.
2. Incubate at 37℃ for 1 hour. Remove the solution, wash with PBS(-), and seed the cells.

Ideal as a matrix for a wide variety of cultured cells, including mesenchymal stem cells (MSCs). With high safety and minimal lot-to-lot variability in performance, it is well-suited for a broad range of applications, including regenerative medicine research.

Human Bone Marrow-Derived MSCs

Cell Adhesion Assay

Media: Serum-free media for MSC
Fibronectin: 2.5 μg/cm² each
Seeding Density: 1.58 x 10³ cells/cm²
Culture Conditions: 37℃、5％ CO₂
Incubation Time: 1 hour

Our fibronectins (plasma fibronectin and cellular fibronectin) were confirmed to promote the adhesion of human bone marrow-derived MSCs to culture vessels.​

BHK21 Cells

Cell Adhesion Assay

Media: Serum-free medium
Fibronectin: 2.5 μg/cm² each
Seeding Density: 7.4×10⁴ cells/cm²
Culture Conditions: 37℃, 5% CO₂
Incubation Time: 40 minutes

Our fibronectins (plasma fibronectin and cellular fibronectin) were confirmed to promote the adhesion of BHK21 cells to culture vessels.