IL-17A ELISA Kits
Interleukin 17A (IL-17A) is an inflammatory cytokine produced primarily by immune cells including Th17 cells. It acts on a diverse range of cells ― including macrophages, fibroblasts, epithelial cells, endothelial cells, and stromal cells ― and efficiently induces the production of inflammatory mediators in these cells. Fujifilm Wako offers an ELISA kit capable of measuring IL-17A in mouse serum and plasma with high sensitivity.
What is IL-17A？
IL-17A is an inflammatory cytokine secreted as an approximately 35 kDa homodimeric glycoprotein, with two 133-amino acid (mouse) subunits linked by disulfide bonds. The IL-17 family consists of six structurally homologous members (IL-17A through F); the term "IL-17" generally refers to IL-17A. Studies have shown that IL-17 is produced by a subset of CD4⁺ T cells distinct from Th1 and Th2 cells, known as Th17 cells. Secreted IL-17A acts on a diverse range of cells — including macrophages, fibroblasts, epithelial cells, endothelial cells, and stromal cells — and induces the expression of inflammatory mediators such as IL-6, IL-8, and various chemokines.
IL-17A has been implicated in the activation of NF-κB signaling, a regulator of inflammatory responses, as well as the MAPK pathway, which mediates intracellular signaling1).
Furthermore, IL-17A plays an important role in various autoimmune diseases including rheumatoid arthritis (RA), inflammatory diseases, allergic reactions, and defense against bacterial infections. As such, it is attracting attention as a novel therapeutic target.
LBIS™ Mouse IL-17A ELISA Kit
LBIS™ Human IL-17A ELISA Kit is a sandwich ELISA kit for the quantitative measurement of mouse IL-17A. It enables highly sensitive quantification of IL-17A in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.
Kit Performance
| Assay target | IL-17A |
|---|---|
| Calibration curve range | 2.06 - 800 pg/mL |
| Analysis sample | Mouse Serum/Plasma (EDTA・Heparin) |
| Sample volume | 25 μL (2x dilution) |
| Measurement duration | Approx. 3 hours and 50 minutes |
| Wavelength | Primary wavelength : 450 nm Reference wavelength : 620 nm |
Example of Calibration Curve
Measurement of IL-17A in Mouse Serum and Plasma
| Sample No. | Serum | Plasma (EDTA) |
Plasma (Heparin) |
|||
|---|---|---|---|---|---|---|
| Fujifilm Wako (pg/mL) |
Company A (pg/mL) |
Fujifilm Wako (pg/mL) |
Company A (pg/mL) |
Fujifilm Wako (pg/mL) |
Company A (pg/mL) |
|
| 1 | 5.75 | N.D. | 96.8 | N.D. | 57.5 | N.D. |
| 2 | 46.6 | N.D. | 4.28 | N.D. | 26.7 | N.D. |
| 3 | 45.2 | 5.14 | 12.4 | N.D. | 47.2 | N.D. |
| 4 | 1.90 (Reference value) |
N.D. | 3.01 | N.D. | 6.88 | N.D. |
| 5 | 53.2 | N.D. | 5.62 | 5.21 | 18.2 | N.D. |
| Proportion of samples successfully (values within the quantification range) |
4/5 samples | 1/5 sample | 5/5 samples | 1/5 sample | 5/5 samples | 0/5 sample |
[Result]
IL-17A was detected even in samples which were below the lower limit of quantification of another manufacture's kit.
Reference
- Gaffen, L.: Nature Reviews Immunology, 9, 556 (2009).
Structure and signalling in the IL-17 receptor family
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IL-17A ELISA Kit (Mouse)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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