ITEA Allergen reagent for ISO4333 testing
Institute of Tokyo Environmental Allergy (ITEA Inc,) sells allergen extracts and ELISA kits for the simple implementation of ISO4333 "Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products".
Japanese cedar pollen
(Cryptomeria japonica)
House Dust Mite
(Dermatophagoides farinae)
Examples of target allergens
| Allergens | Origin |
|---|---|
| Cry j 1 | Japanese cedar pollen (Cryptomeria japonica) |
| Der f 1 | house dust mite (Dermatophagoides farinae) feces |
| Der p 1 | house dust mite (Dermatophagoides pteronyssinus) feces |
- ISO 4333: "Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products".
- July, 2022, a method for measuring the reduction of pollen, mite and other proteins derived from pollen, mite and other sources on textile products was issued. (ISO 4333Textiles - Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products)
This is a standardized test method for evaluating the degree to which allergen activity, a protein derived from pollen and mites, can be reduced in textile products processed against pollen and mites, which are the causes of allergies.
Allergen Extracts
House Dust Mite Extract (Contains 50% glycerol)
This is a crude extract of mite allergens that can be used as an antigen for ISO 4333: "Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products”.
Specifications
- Crude extract, Containing 50% glycerol.
- Contain preservatives
- #4-DF-004(Left) : 15 µg/mL x 1tube（1 mL/tube）
- #4-DP-004 : 7.5 µg/mL x 2tubes（1 mL/tube）
- Included：Product information, Certificate of Analysis
Usage
4-DF-004 should be diluted 1000 times with PBST before use.
4-DP-004 should be diluited 500 times with PBST before use.
| Product Name | Specifications | Package Size | Manufacture Code |
|---|---|---|---|
| House Dust Mite Extract, Whole Culture (Df) (Contains 50% glycerol) |
Der f 1, 15 µg | １ｍL | 4-DF-004 |
| House Dust Mite Extract, Whole Culture (Dp) (Contains 50% glycerol) |
Der p 1, 15 µg | １ｍL×2 | 4-DP-004 |
＊ Whole Culture: Extracts from growth media (including feed and excreta)
Japanese Cedar Pollen Extract (Contains 50% glycerol)
This is a crude extract Japanese Cedar pollen allergen that can be used as an antigen for ISO 4333: “Determination of the reduction of pollen and mite-derived proteins on textile products”.
Specifications
- Crude extract, Containing 50% glycerol.
- Contain preservatives
- 7.5 µg/mL x 2 tubes(1mL/tube)
- Included：Product information, Certificate of Analysis
Usage
Dilute 500 times with PBST for use.
| Product Name | Specifications | Package Size | Manufacture Code |
|---|---|---|---|
| Japanese Cedar Pollen Extract (Contains 50% glycerol) |
Cry j 1, 15 µg | 1mL×2 | 4-CJ-004 |
ELISA Kit
ELISA Kits (Antibody coated microplates type)
This is a ready-to-use immunoassays ELISA kit that includes a 96-well strip plate with pre-coated antibodies for the target allergen.
Components (Example)
- Capture antibody coated microplate
- Standard solution (lyophilized)
- Enzyme-labeled antibody
- TMB soluton
- Stop solution
- Dilute solution
- Washing solution (20x concentrated)
- Microplate seals
- Instruction Manual
| Product Name | Manufacture Code |
|---|---|
| ITEA Der f 1 ELISA Kit | 1-DF1-001 |
| ITEA Der p 1 ELISA Kit | 1-DP1-001 |
| ITEA Der f 1 ELISA Kit, High Sensitive | 1-DFH-001 |
| ITEA Der f 2 ELISA Kit | 1-DF2-001 |
| ITEA Der p 2 ELISA Kit | 1-DP2-001 |
| ITEA Cry j 1 ELISA Kit | 1-CJ1-001 |
ELISA Kits (Self-build type)
Components
- Capture monoclonal antibody for coating
- Standard solution(Lyophilized)
- labeled monoclonal antibody
- Instruction manual
Biotin-labeled
| Product Name | Manufacture Code |
|---|---|
| ITEA Cry j 1 ELISA Self-build Kit (Biotin-labeled) | 1-CJ1-002 |
| ITEA Der f 1 ELISA Self-build Kit (Biotin-labeled) | 1-DF1-002 |
| ITEA Der p 1 ELISA Self-build Kit (Biotin-labeled) | 1-DP1-002 |
| ITEA Der f 2 ELISA Self-build Kit (Biotin-labeled) | 1-DF2-002 |
HRP-Labeled
| Product Name | Manufacture Code |
|---|---|
| ITEA Cry j 1 ELISA Self-build Kit (HRP-labeled) | 1-CJ1-003 |
| ITEA Der f 1 ELISA Self-build Kit (HRP-labeled) | 1-DF1-003 |
| ITEA Der p 1 ELISA Self-build Kit (HRP-labeled) | 1-DP1-003 |
| ITEA Der f 2 ELISA Self-build Kit (HRP-labeled) | 1-DF2-003 |
Q&A
- Can ITEA extracts be used with kits made by other companies?
- Yes, they can. However, depending on the kit type, the allergen concentration stated in the specification of ITEA may differ slightly.
- Does the preservative contained in the extract affect the ELISA measurement system?
- Proclin® 950 is added as preservatives, which has no effect on the enzymes used in routine ELISA. Products without preservatives are also available. Please feel free to consult us.
Product List
- Open All
- Close All
Allergen Extracts
ELISA Kits, Antibody coated microplates type
ELISA Kits, Self-build type（HRP-labeled）
ELISA Kits, Self-build type（Biotin-labeled）
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.