Allergen extracts and ELISA kits to easily perform ISO 4333: “Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products”.

ITEA Allergen reagent for ISO4333 testing

logo ITEA

Institute of Tokyo Environmental Allergy (ITEA Inc,) sells allergen extracts and ELISA kits for the simple implementation of ISO4333 "Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products".

cedar pollen

Japanese cedar pollen
(Cryptomeria japonica)

House Dust Mite

House Dust Mite
(Dermatophagoides farinae)

Examples of target allergens

Allergens Origin
Cry j 1 Japanese cedar pollen (Cryptomeria japonica)
Der f 1 house dust mite (Dermatophagoides farinae) feces
Der p 1 house dust mite (Dermatophagoides pteronyssinus) feces
ISO 4333: "Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products".
July, 2022, a method for measuring the reduction of pollen, mite and other proteins derived from pollen, mite and other sources on textile products was issued. (ISO 4333Textiles - Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products)
This is a standardized test method for evaluating the degree to which allergen activity, a protein derived from pollen and mites, can be reduced in textile products processed against pollen and mites, which are the causes of allergies.

Allergen Extracts

House Dust Mite Extract (Contains 50% glycerol)

This is a crude extract of mite allergens that can be used as an antigen for ISO 4333: "Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products”.

House Dust Mite Extract (Contains 50% glycerol)

Specifications

  • Crude extract, Containing 50% glycerol.
  • Contain preservatives
  • #4-DF-004(Left) : 15 µg/mL x 1tube(1 mL/tube)
  • #4-DP-004 : 7.5 µg/mL x 2tubes(1 mL/tube)
  • Included:Product information, Certificate of Analysis

Usage

4-DF-004 should be diluted 1000 times with PBST before use.
4-DP-004 should be diluited 500 times with PBST before use.

Product Name​ Specifications Package Size Manufacture Code
House Dust Mite Extract, Whole Culture (Df)
(Contains 50% glycerol)
Der f 1, 15 µg 1mL 4-DF-004
House Dust Mite Extract, Whole Culture (Dp)
(Contains 50% glycerol)
Der p 1, 15 µg 1mL×2 4-DP-004

* Whole Culture: Extracts from growth media (including feed and excreta)

Japanese Cedar Pollen Extract (Contains 50% glycerol)

This is a crude extract Japanese Cedar pollen allergen that can be used as an antigen for ISO 4333: “Determination of the reduction of pollen and mite-derived proteins on textile products”.

 Japanese Cedar Pollen Extract (Contains 50% glycerol)

Specifications

  • Crude extract, Containing 50% glycerol.
  • Contain preservatives
  • 7.5 µg/mL x 2 tubes(1mL/tube)
  • Included:Product information, Certificate of Analysis

Usage

Dilute 500 times with PBST for use.

Product Name​  Specifications Package Size Manufacture Code
Japanese Cedar Pollen Extract
(Contains 50% glycerol)
 Cry j 1, 15 µg 1mL×2 4-CJ-004

ELISA Kit

ELISA Kits (Antibody coated microplates type)​

This is a ready-to-use immunoassays ELISA kit that includes a 96-well strip plate with pre-coated antibodies for the target allergen.​

ELISA Kits (Antibody coated microplates type)​

Components (Example)

  • Capture antibody coated microplate
  • Standard solution (lyophilized)
  • Enzyme-labeled antibody
  • TMB soluton
  • Stop solution
  • Dilute solution
  • Washing solution (20x concentrated)
  • Microplate seals
  • Instruction Manual
Product Name Manufacture Code
ITEA Der f 1 ELISA Kit 1-DF1-001
ITEA Der p 1 ELISA Kit 1-DP1-001
ITEA Der f 1 ELISA Kit, High Sensitive 1-DFH-001
ITEA Der f 2 ELISA Kit 1-DF2-001
ITEA Der p 2 ELISA Kit 1-DP2-001
ITEA Cry j 1 ELISA Kit 1-CJ1-001

ELISA Kits (Self-build type)​

ELISA Kits (Self-build type)​

Components

  • Capture monoclonal antibody for coating
  • Standard solution(Lyophilized)
  • labeled monoclonal antibody
  • Instruction manual

Biotin-labeled

Product Name Manufacture Code
ITEA Cry j 1 ELISA Self-build Kit (Biotin-labeled) 1-CJ1-002
ITEA Der f 1 ELISA Self-build Kit (Biotin-labeled) 1-DF1-002
ITEA Der p 1 ELISA Self-build Kit (Biotin-labeled) 1-DP1-002
ITEA Der f 2 ELISA Self-build Kit (Biotin-labeled) 1-DF2-002

HRP-Labeled

Product Name Manufacture Code
ITEA Cry j 1 ELISA Self-build Kit (HRP-labeled) 1-CJ1-003
ITEA Der f 1 ELISA Self-build Kit (HRP-labeled) 1-DF1-003
ITEA Der p 1 ELISA Self-build Kit (HRP-labeled) 1-DP1-003
ITEA Der f 2 ELISA Self-build Kit (HRP-labeled) 1-DF2-003

Q&A

Can ITEA extracts be used with kits made by other companies?
Yes, they can. However, depending on the kit type, the allergen concentration stated in the specification of ITEA may differ slightly.
Does the preservative contained in the extract affect the ELISA measurement system?
Proclin® 950 is added as preservatives, which has no effect on the enzymes used in routine ELISA. Products without preservatives are also available. Please feel free to consult us.

Product List

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Allergen Extracts

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ELISA Kits, Antibody coated microplates type

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ELISA Kits, Self-build type(HRP-labeled)

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ELISA Kits, Self-build type(Biotin-labeled)

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

+49-2131-311-271

Reception hours: Mon-Fri 9:00 - 15:00 (CET)For other hours than the above, please contact us via the inquiry form.