Kuni-Grow+ ® series
Kuni-Grow+, developed by Kunimine Industries Co.Ltd., is a reagent for cell culture that composed mainly by inorganic layered compounds (clay). Simply by adding it to your culture medium, it adsorbs growth factors (serum proteins, etc.) in the medium and accelerates the formation of spheroids by acting on cells. Research to date has confirmed spheroid formation in a variety of cancer cell lines. We offer four types of reagents with different mineral species, and we have prepared a trial kit so that you can first select the most suitable reagent for your target cell lines.
Features
- No special culture media or culture containers are required.
- Able to store at room temperature.
- Effectiveness has been confirmed in various cancer cell lines.
- Spheroid formation is possible in a short time (several days to a week).
- Observation of time lapse is possible because spheroid formations are distributed throughout the entire culture and do not move much.
Magnified image of spheroid of HT29 cell
-
*Microscopic image -
*Electron microscope image
How to use
- Add Kuni-Grow+ to your culture medium at a ratio of 98(medium):2(reagent) and mix well.
- Filter through a syringe filter (5 μm pore size) to remove aggregates.
Note: This is not mandatory procedure, but is recommended. - Add an equal volume of cell suspension to the culture plate after dispensing, then, start the culture.
Note: Exchanging culture media is the same method as normal planar culture.
Examples
Example of culture with HT29 cells (after 5 days)
- Culture medium:
- RPMI-1640, Serum 10% FCS
- Plate:
- Adherent cell culture plate
Overall, spheroid formation was observed after Kuni-Grow+ is added.
Comparison with dedicated medium in U-87MG cells (after 5 days)
- Medium:
- RPMI-1640, serum 10% FCS
- Plate:
- Adherent cell culture plate
In both cases, spheroids are distributed throughout, but spheroid size is larger when Kuni-Grow+ is added.
Cell lines with proven efficacy (as of March 2024)
| Cell type | Cell line | Origin |
|---|---|---|
| Glioblastoma | LN-229 | Human Glioblastoma |
| KNS-81 | Human Glioblastoma | |
| LN-299 | Human Glioblastoma | |
| U-251MG | Human Glioblastoma | |
| U-87MG | Human Glioblastoma | |
| Kidney Cancer | 786-O | Human Kidney Cancer |
| ACHN | Human Renal Line Carcinoma | |
| Caki-1 | Human Kidney Cancer | |
| Caki-2 | Human Kidney Cancer | |
| Colorectal Cancer | DLD1 | Adenocarcinoma of the Human Colon |
| HCT 116 | Adenocarcinoma of the Human Colon | |
| HT29 | Adenocarcinoma of the Human Colon | |
| SW620 | Adenocarcinoma of the Human Colon | |
| Pancreatic Cancer | AsPC-1 | Human Metastatic Pancreatic Adenocarcinoma |
| KLM-1 | Human Pancreatic Cancer | |
| MIA PaCa-2 | Human Pancreatic Cancer | |
| PANC-1 | Human Pancreatic Adenocarcinoma | |
| Ovarian Cancer | A2780 | Human Ovarian Cancer |
| Caov-3 | Human Ovarian Cancer | |
| SK-OV-3 | Human Ovarian Adenoma | |
| Bladder Cancer | 5637 | Human Bladder Cancer |
| UM-UC-3 | Human Bladder Cancer | |
| Lung Cancer | A549 | Human Lung Cancer |
| Neuroblastoma | SH-SY5Y | Human Human Neuroblastoma |
Lineup
Trial kits (4 types in total, 1mL each) are available, so please select the optimal reagent for your target cell line.
| Product code | Product Name | Volume |
|---|---|---|
| 192000 | Kuni-Grow+_Trial Kit | 1mLx4 |
| 192010 | Kuni-Grow+_B (Blue label) | 5mL |
| 192020 | Kuni-Grow+_Y (Yellow label) | 5mL |
| 192030 | Kuni-Grow+_G (Green label) | 5mL |
| 192040 | Kuni-Grow+_R (Red label) | 5mL |
Note: Each grade (B, Y, G, R) has different mineral species. Please use according to the compatibility with the culture cell type.
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.