GC Lymphotec iStock
GC Lymphotec Inc.'s iStock is a cell cryopreservation medium that contains no serum or non-human animal-derived components and is suitable for human ES/iPS cells and regenerative medicine research.
iStock ensures long-term stability. Collected cells can be suspended in this product and simply quick-frozen in an ultra-low temperature freezer for long-term storage. Cells can also be frozen and kept at -80oC for about 12 hours and then stored in liquid nitrogen.
Features
- Free of serum and animal-derived components
- No need for a programmed freezer
- No need to prepare a preservation medium
- Suitable for quick-freezing and long-term storage at -80℃
- 3-year shelf life under refrigeration
- Approval from PMDA as raw materials can be used for regenerative medicine product
Performance comparison between iStock and other cryopreservation media
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Human peripheral blood mononuclear cells (PBMC)
PBMCs were isolated from the blood of 9 healthy subjects who gave informed consent and cultured for 4 days in GC Lymphotec lymphocyte culture medium in OKT3 antibody-coated flasks.
Cultured cells were resuspended to 1.5×107 cells/mL in a serum-containing preservation medium or iStock, and frozen at -80℃ by the slow-freezing method. The cells were transferred to a liquid nitrogen tank on the next day, stored for 7 days, and then thawed to compare the recovery rate. (Recovery rate = number of cells after thawing / number of cells before freezing x 100%)⇒ Recovery rate was equivalent to between iStock and a serum-containing cryopreservation medium.
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Mouse ES cells
Cultured mouse ES cells were resuspended to 4 x 106 cells/mL in 10% DMSO/Medium, iStock, product A, or product B. One mL of each cell suspension was dispensed into cryopreservation tubes (n=4) and frozen at -80℃ without using a freezing container. The cells were transferred to liquid nitrogen on the next day and stored for 1 week. The cells were quickly thawed at 37℃, centrifuged, and resuspended in the culture medium to determine the number of viable cells to compare the recovery rate.
⇒ Recovery rate was higher with iStock than other cryopreservation media.
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Human iPS cells
Human iPS cells were frozen at the concentration of 3×106 cells/tube (n=3) and stored in liquid nitrogen.
After thawing, cells were seeded in culture vessels and counted 3 days later.⇒ Cell growth after thawing was significantly better with iStock than with competitor’s product A
How to Use iStock
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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